TY - CHAP A1 - Daus, Andreas A1 - Hasenkopf, Matthias A1 - Thielemann, Christiane T1 - Novel 3D cell culture systems for electromagnetic exposure studies T2 - BioEM 2009, Davos, Switzerland KW - Zellkultur Y1 - 2009 ER - TY - JOUR A1 - Trent, Davis A1 - Thielemann, Christiane A1 - Monici, Monica T1 - How are cell and tissue structure and function influenced by gravity and what are the gravity perception mechanisms? JF - npj Microgravity N2 - Progress in mechanobiology allowed us to better understand the important role of mechanical forces in the regulation of biological processes. Space research in the field of life sciences clearly showed that gravity plays a crucial role in biological processes. The space environment offers the unique opportunity to carry out experiments without gravity, helping us not only to understand the effects of gravitational alterations on biological systems but also the mechanisms underlying mechanoperception and cell/tissue response to mechanical and gravitational stresses. Despite the progress made so far, for future space exploration programs it is necessary to increase our knowledge on the mechanotransduction processes as well as on the molecular mechanisms underlying microgravity-induced cell and tissue alterations. This white paper reports the suggestions and recommendations of the SciSpacE Science Community for the elaboration of the section of the European Space Agency roadmap “Biology in Space and Analogue Environments” focusing on “How are cells and tissues influenced by gravity and what are the gravity perception mechanisms?” The knowledge gaps that prevent the Science Community from fully answering this question and the activities proposed to fill them are discussed. KW - Zellkultur KW - Schwere KW - Raumfahrtbiologie Y1 - 0024 UR - https://rdcu.be/dyCjL U6 - https://doi.org/10.1038/s41526-024-00357-9 VL - 2014 IS - 10 / 16 SP - 1 EP - 7 ER - TY - JOUR A1 - Heselich, Anja A1 - Frieß, Johannes A1 - Ritter, Sylvia A1 - Benz, Naja A1 - Layer, Paul A1 - Thielemann, Christiane T1 - High LET radiation shows no major cellular and functional effects on primary cardiomyocytes in vitro JF - Life Sciences in Space Research N2 - It is well known that ionizing radiation causes adverse effects on various mammalian tissues. However, there is little information on the biological effects of heavy ion radiation on the heart. In order to fill this gap, we systematically examined DNA-damage induction and repair, as well as proliferation and apoptosis in avian cardiomyocyte cultures irradiated with heavy ions such as titanium and iron, relevant for manned space-flight, and carbon ions, as used for radiotherapy. Further, and to our knowledge for the first time, we analyzed the effect of heavy ion radiation on the electrophysiology of primary cardiomyocytes derived from chicken embryos using the non-invasive microelectrode array (MEA) technology. As electrophysiological endpoints beat rate and field action potential duration were analyzed. The cultures clearly exhibited the capacity to repair induced DNA damage almost completely within 24 h, even at doses of 7 Gy, and almost completely recovered from radiation-induced changes in proliferative behavior. Interestingly, no significant effects on apoptosis could be detected. Especially the functionality of primary cardiac cells exhibited a surprisingly high robustness against heavy ion radiation, even at doses of up to 7 Gy. In contrast to our previous study with X-rays the beat rate remained more or less unaffected after heavy ion radiation, independently of beam quality. The only change we could observe was an increase of the field action potential duration of up to 30% after titanium irradiation, diminishing within the following three days. This potentially pathological observation may be an indication that heavy ion irradiation at high doses could bear a long-term risk for cardiovascular disease induction. KW - Cardiomyocytes KW - Heavy ion irradiation KW - Space radiation KW - Electrophysiology KW - MEA-technology KW - Zellkultur KW - Herzmuskelzelle Y1 - 2018 U6 - https://doi.org/10.1016/j.lssr.2018.01.001 VL - 2018 IS - 16 SP - 93 EP - 100 ER - TY - JOUR A1 - Daus, Andreas A1 - Goldhammer, Michael A1 - Layer, Paul A1 - Thielemann, Christiane T1 - Electromagnetic exposure of scaffold-free three-dimensional cell culture systems JF - Bioelectromagnetics KW - Zellkultur KW - Herzmuskelzelle Y1 - 2014 U6 - https://doi.org/10.1002/bem.20649 SN - 1521-186X VL - 32 IS - 5 SP - 351 EP - 359 ER - TY - JOUR A1 - Thielemann, Christiane A1 - Krstić, Nenad A1 - Jüttner, Jens A1 - Giegerich, Lars A1 - Mayer, Margot A1 - Knuth, Monika A1 - Müller, Achim T1 - 3D printed biosensor for continuous glucose measurement in cell cultures JF - Annals of 3D Printed Medicine N2 - A novel 3D-printed glucose sensor is presented for cell culture application. Glucose sensing was performed using a fluorescence resonance energy transfer (FRET)-based assay principle based on ConA and dextran. Both molecules are encapsulated in alginate microspheres and embedded in the UV-curable, stable hydrogel polyvinyl alcohol (PVA). The rheology of the formulation was adapted to obtain good properties for an extrusion-based printing process. The printed sensor structures were tested for their ability to detect glucose in vitro. A proportional increase in fluorescence intensity was observed in a concentration range of 0 - 2 g/L glucose. Tests with HEK cell cultures also showed good cell compatibility and excellent adhesion properties on plasma-treated Petri dishes. The printed sensors were able to detect the glucose decay associated with the metabolic activities of the fast-growing HEK cells in the cell culture medium over ten days. The proof-of-principle study shows that metabolic processes in cell cultures can be monitored with the new printed sensor using a standard fluorescence wide-field microscope. KW - Bioprinting, FRET, Enzyme-free glucose sensor, PVA hydrogel KW - Biosensor KW - 3D-Druck KW - Zellkultur Y1 - 2023 U6 - https://doi.org/https://doi.org/10.1016/j.stlm.2023.100111 VL - 2023 IS - - SP - - EP - - ER -