@inproceedings{RitterDuranteHelmetal.2012, author = {Ritter, Sylvia and Durante, Marco and Helm, Alexander and Steger, Frederik and Daus, Andreas and Frieß, Johannes}, title = {Assessment of cardiotoxicity of heavy ions in vitro: generation and electrophysiology of cardiomyocytes}, series = {39th COSPAR Scientific Assembly, Mysore, India}, booktitle = {39th COSPAR Scientific Assembly, Mysore, India}, year = {2012}, subject = {Herzmuskelzelle}, language = {en} } @phdthesis{Friess2016, author = {Frieß, Johannes}, title = {Einfluss ionisierender Strahlung auf die elektrophysiologischen Eigenschaften kardialer Zellen}, school = {Technische Hochschule Aschaffenburg}, year = {2016}, abstract = {Die zentrale Fragestellung der Strahlenbiologie behandelt die Wirkung von ionisierender Strahlung auf biologische Systeme. Zwar sind die bei Exposition mit ionisierender Strahlung entstehenden Gesundheitsrisiken bekannt, dennoch sind die Strahlungseffekte auf das Herz immer noch nicht sehr gut verstanden. Dabei ist die Exposition mit hohen Dosen ionisierender Strahlung ein anerkannter Risikofaktor in Bezug auf kardiovaskul{\"a}re Erkrankungen.}, subject = {Elektrophysiologie}, language = {de} } @article{HeselichFriessRitteretal.2018, author = {Heselich, Anja and Frieß, Johannes and Ritter, Sylvia and Benz, Naja and Layer, Paul and Thielemann, Christiane}, title = {High LET radiation shows no major cellular and functional effects on primary cardiomyocytes in vitro}, series = {Life Sciences in Space Research}, volume = {2018}, journal = {Life Sciences in Space Research}, number = {16}, doi = {10.1016/j.lssr.2018.01.001}, pages = {93 -- 100}, year = {2018}, abstract = {It is well known that ionizing radiation causes adverse effects on various mammalian tissues. However, there is little information on the biological effects of heavy ion radiation on the heart. In order to fill this gap, we systematically examined DNA-damage induction and repair, as well as proliferation and apoptosis in avian cardiomyocyte cultures irradiated with heavy ions such as titanium and iron, relevant for manned space-flight, and carbon ions, as used for radiotherapy. Further, and to our knowledge for the first time, we analyzed the effect of heavy ion radiation on the electrophysiology of primary cardiomyocytes derived from chicken embryos using the non-invasive microelectrode array (MEA) technology. As electrophysiological endpoints beat rate and field action potential duration were analyzed. The cultures clearly exhibited the capacity to repair induced DNA damage almost completely within 24 h, even at doses of 7 Gy, and almost completely recovered from radiation-induced changes in proliferative behavior. Interestingly, no significant effects on apoptosis could be detected. Especially the functionality of primary cardiac cells exhibited a surprisingly high robustness against heavy ion radiation, even at doses of up to 7 Gy. In contrast to our previous study with X-rays the beat rate remained more or less unaffected after heavy ion radiation, independently of beam quality. The only change we could observe was an increase of the field action potential duration of up to 30\% after titanium irradiation, diminishing within the following three days. This potentially pathological observation may be an indication that heavy ion irradiation at high doses could bear a long-term risk for cardiovascular disease induction.}, subject = {Zellkultur}, language = {en} } @techreport{MaternaHelmHesseletal.2012, author = {Materna, Miriam and Helm, Alexander and Hessel, Petra and Frieß, Johannes and Thielemann, Christiane and Pignalosa, Diana and M{\"a}ntele, Werner and Durante, Marco and Ritter, Sylvia}, title = {First electrophysiological studies on mouse embryonic stem cell-derived cardiomyocytes}, organization = {GSI Helmholtzzentrum f{\"u}r Schwerionenforschung}, year = {2012}, subject = {Herzmuskelzelle}, language = {en} } @inproceedings{FriessRitterDausetal.2013, author = {Frieß, Johannes and Ritter, Sylvia and Daus, Andreas and Layer, Paul and Thielemann, Christiane and Heselich, Anja}, title = {Electrophysiologic and Molecular Characteristics of Cardiomyocytes after Heavy Ion Irradiation in the frame of the ESA IBER-10 Program}, series = {Heavy Ion in Therapie and Space Radiation Symposium (HITSRS2013), 2013, Chiba, Japan}, booktitle = {Heavy Ion in Therapie and Space Radiation Symposium (HITSRS2013), 2013, Chiba, Japan}, year = {2013}, subject = {Herzmuskelzelle}, language = {en} } @inproceedings{StegerFriessDausetal.2012, author = {Steger, Frederik and Frieß, Johannes and Daus, Andreas and Ritter, Sylvia and Thielemann, Christiane}, title = {Analysis of electrophysiological characteristics of cardiomyocytes following radiation exposure}, series = {8th International Meeting on Substrate-Integrated Microelectrode Arrays}, booktitle = {8th International Meeting on Substrate-Integrated Microelectrode Arrays}, issn = {2194-5519}, doi = {10.13140/RG.2.1.4153.0400}, pages = {153}, year = {2012}, subject = {Herzmuskelzelle}, language = {en} } @inproceedings{ThielemannRitterLayeretal.2011, author = {Thielemann, Christiane and Ritter, Sylvia and Layer, Paul and Frieß, Johannes and Daus, Andreas and Steger, Frederik and Heselich, Anja}, title = {Cellular effects of space radiation with relevance to cardiovascular diseases}, series = {Life in Space for Life on Earth}, booktitle = {Life in Space for Life on Earth}, pages = {560}, year = {2011}, subject = {Kardiovaskul{\"a}re Krankheit}, language = {en} } @techreport{HelmFrankFriessetal.2013, author = {Helm, Alexander and Frank, Simon and Frieß, Johannes and Thielemann, Christiane and Durante, Marco and Ritter, Sylvia}, title = {Purified cultures of mouse embryonic stem cell-derived cardiomyocytes of electrophysiological studies with ionizing radiation}, organization = {GSI Helmholtzzentrum f{\"u}r Schwerionenforschung}, year = {2013}, subject = {Herzmuskelzelle}, language = {en} } @article{FriessHeselichRitteretal.2015, author = {Frieß, Johannes and Heselich, Anja and Ritter, Sylvia and Haber, Angelina and Kaiser, Nicole and Layer, Paul and Thielemann, Christiane}, title = {Electrophysiologic and cellular characteristics of cardiomyocytes after X-ray irradiation}, series = {Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis}, volume = {2015}, journal = {Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis}, number = {777}, pages = {1 -- 10}, year = {2015}, subject = {Herzmuskelzelle}, language = {en} } @techreport{FriessHeselichRitteretal.2013, author = {Frieß, Johannes and Heselich, Anja and Ritter, Sylvia and Layer, Paul and Thielemann, Christiane}, title = {Effect of X-rays and titanium ions on cardiomyocyte cultures}, organization = {GSI Helmholtzzentrum f{\"u}r Schwerionenforschung}, year = {2013}, subject = {Herzmuskelzelle}, language = {en} } @techreport{FriessHeselichRitteretal.2012, author = {Frieß, Johannes and Heselich, Anja and Ritter, Sylvia and Daus, Andreas and Layer, Paul and Thielemann, Christiane}, title = {Electrophysiological Effects of Heavy Ion Irradiation on Cardiomyocytes}, organization = {GSI Helmholtzzentrum f{\"u}r Schwerionenforschung}, year = {2012}, subject = {Herzmuskelzelle}, language = {en} } @inproceedings{FriessHeselichRitteretal.2013, author = {Frieß, Johannes and Heselich, Anja and Ritter, Sylvia and Thielemann, Christiane}, title = {Electrophysiological characteristics of cardiomyocytes after heavy ion exposure}, series = {Jahrestagung der Gesellschaft f{\"u}r biologische Strahlenforschung}, booktitle = {Jahrestagung der Gesellschaft f{\"u}r biologische Strahlenforschung}, address = {Darmstadt, Deutschland}, year = {2013}, subject = {Herzmuskelzelle}, language = {de} } @inproceedings{ThielemannRitterLayeretal.2012, author = {Thielemann, Christiane and Ritter, Sylvia and Layer, Paul and Frieß, Johannes and Daus, Andreas and Steger, Frederik and Heselich, Anja}, title = {IBER10 - Cellular effects of space radiation with relevance to cardiovascular diseases}, series = {European Space Agency - Life in Space for Life on Earth}, booktitle = {European Space Agency - Life in Space for Life on Earth}, address = {Aberdeen, Schottland, UK}, year = {2012}, subject = {Kosmische Strahlung}, language = {en} } @techreport{StegerRitterDausetal.2011, author = {Steger, Frederik and Ritter, Sylvia and Daus, Andreas and Frieß, Johannes and Thielemann, Christiane}, title = {Analysis of electrophysiological characteristics of cardiomyocytes following radiation exposure}, organization = {GSI Helmholtzzentrum f{\"u}r Schwerionenforschung}, year = {2011}, subject = {Herzmuskelzelle}, language = {en} } @techreport{HelmFrankFriessetal.2014, author = {Helm, Alexander and Frank, Simon and Frieß, Johannes and Thielemann, Christiane and Durante, Marco and Ritter, Sylvia}, title = {Electrophysiological response of mouse embryonic stem cell-derived cardiomyocytes after X-ray and C-ion exposure}, series = {GSI SCIENTIFIC REPORT 2014}, journal = {GSI SCIENTIFIC REPORT 2014}, doi = {10.15120/GR-2015-1-APPA-HEALTH-21}, year = {2014}, subject = {Embryonale Stammzelle}, language = {en} } @techreport{FriessHeselichRitteretal.2014, author = {Frieß, Johannes and Heselich, Anja and Ritter, Sylvia and Layer, Paul and Thielemann, Christiane}, title = {Combined effects of ionizing radiation and cardio-active drugs on human iPSC-derived cardiomyocytes}, series = {GSI SCIENTIFIC REPORT 2014}, journal = {GSI SCIENTIFIC REPORT 2014}, doi = {10.15120/GR-2015-1-APPA-HEALTH-37}, year = {2014}, subject = {Herzmuskelzelle}, language = {en} }