@article{WagnerHildenbrandSpoerietal.2006, author = {Wagner, Christian and Hildenbrand, Georg and Sp{\"o}ri, Udo and Cremer, Christoph}, title = {Beyond nanosizing: an approach to shape analysis of fluorescent nanostructures by SMI-microscopy}, series = {Optik}, volume = {117}, journal = {Optik}, number = {1}, publisher = {Elsevier BV}, issn = {0030-4026}, doi = {https://doi.org/10.1016/j.ijleo.2005.05.006}, pages = {26 -- 32}, year = {2006}, subject = {Fluoreszenzmikroskopie}, language = {en} } @article{FaillaAlbrechtSpoerietal.2003, author = {Failla, Antonio Virgillo and Albrecht, Benno and Sp{\"o}ri, U. and Schweitzer, A. and Kroll, A. and Hildenbrand, Georg and Bach, M. and Cremer, Christoph}, title = {Nanostructure Analysis Using Spatially Modulated Illumination Microscopy}, series = {Complexus}, volume = {1}, journal = {Complexus}, number = {2}, publisher = {S. Karger AG}, issn = {1424-8492}, doi = {https://doi.org/10.1159/000070464}, pages = {77 -- 88}, year = {2003}, abstract = {For an improved understanding of cellular processes, it is highly desirable to develop light optical methods for the analysis of biological nanostructures and their dynamics in the interior of three-dimensionally (3D) conserved cells. Here, important structural parameters to be considered are the topology, i.e. the mutual positions and distances, as well as the sizes of the constituting subunits. This has become possible by the development of a novel method of far-field light fluorescence microscopy, spatially modulated illumination (SMI) microscopy. Using this approach, axial distances between fluorescence-labeled targets can be measured with an accuracy close to 1 nm; their sizes can be determined down to a few tens of nanometers. This approach can be extended to the determination of 3D positions and mutual 3D distances and sizes of any number of small objects/subunits that can be discriminated due to their spectral signatures. Consequently, the new approach allows an 'in situ nanostructure elucidation, until now regarded to be beyond the possibilities of far-field light microscopy. Application examples discussed are: colocalization/nanosizing and topological analysis of large protein-protein complexes, of nucleic acid-protein complexes (such as transcription factories), or of the highly complex DNA-protein nanostructures of which active/ inactive gene regions in the eukaryotic cell nucleus are constituted.}, subject = {Fluoreszenzmikroskopie}, language = {en} } @article{MuellerLemmermannKaufmannetal.2014, author = {M{\"u}ller, Patrick and Lemmermann, Niels A. and Kaufmann, Rainer and Gunkel, Manuel and Paech, Daniel and Hildenbrand, Georg and Holtappels, Rafaela and Cremer, Christoph and Hausmann, Michael}, title = {Spatial distribution and structural arrangement of a murine cytomegalovirus glycoprotein detected by SPDM localization microscopy}, series = {Histochemistry and Cell Biology}, volume = {142}, journal = {Histochemistry and Cell Biology}, number = {1}, publisher = {Springer Science and Business Media LLC}, issn = {0948-6143}, doi = {https://doi.org/10.1007/s00418-014-1185-2}, pages = {61 -- 67}, year = {2014}, subject = {Fluoreszenzmikroskopie}, language = {en} } @article{HildenbrandRappSpoerietal.2005, author = {Hildenbrand, Georg and Rapp, Alexander and Sp{\"o}ri, Udo and Wagner, Christian and Cremer, Christoph and Hausmann, Michael}, title = {Nano-Sizing of Specific Gene Domains in Intact Human Cell Nuclei by Spatially Modulated Illumination Light Microscopy}, series = {Biophysical Journal}, volume = {88}, journal = {Biophysical Journal}, number = {6}, publisher = {Elsevier BV}, issn = {0006-3495}, doi = {https://doi.org/10.1529/biophysj.104.056796}, pages = {4312 -- 4318}, year = {2005}, subject = {Genom}, language = {en} }