@misc{LeeBobkovaGieretal.2019, author = {Lee, Jin-Ho and Bobkova, Elizaveta and Gier, Theresa and Gote, Martin and Schmidt-kaler, Fanz and Brieger, Emily and Maus, Emanuel and Krufczik, Matthias and Chojowski, Robert and Korn, Friederike and Sarah, Schumann and Scherthan, Harry and Falkova, Iva and Falk, Martin and Hausmann, Michael and Hildenbrand, Georg}, title = {Mechanisms and Challenges for Understanding Radiation Induced Changes in Chromatin Nanoarchitecture}, series = {DRO 2018}, journal = {DRO 2018}, doi = {https://doi.org/10.13140/RG.2.2.31391.20647}, year = {2019}, abstract = {The three-dimensional architecture of genomes acts as an additional level of mode for fundamental biological processes such as DNA damage response. In this context, nanoprobing and super-resolution microscopy are powerful methods for structural analyses of genomic targets in native chromatin of single cells at resolutions of single antibodies, proteins, histones, short DNA stretches, etc. We used multi-color chromatin nanoprobing and single molecule localization microscopy of established DNA damage and chromatin markers in 3D-conserved nuclei of different cell types exposed to various types and doses of ionizing radiation. Similarly, effect of gold nanoparticles on extent and direction of cellular radiation response was assessed. Our studies revealed the nanoarchitecture of damage foci with respect to γH2AX, Mre11 or 53BP1 and their molecular rearrangements during repair processes. Nanoscopy of genomic Alu resulted in linear-quadratic dose-effects for low to higher dose ranges and in changes of H3K9me3 distribution around Alu clusters upon radiation exposure. Preliminary results show post-irradiation time dependent changes in Alu chromatin. Moreover, gold nanoparticles incorporated into cells seem to act by an interplay of radiation enhancement and chromatin remodeling leading to altered radiosensitivity. Our results contribute to the understanding of cellular radiation responses, thereby laying the basis for improved biological dosimetry and radiotherapies in future.}, subject = {Genom}, language = {en} } @article{NgwaBoatengKumaretal.2017, author = {Ngwa, Wilfred and Boateng, Francis and Kumar, Rajiv and Irvine, Darrell J. and Formenti, Silvia and Ngoma, Twalib and Herskind, Carsten and Veldwijk, Marlon R. and Hildenbrand, Georg and Hausmann, Michael and Wenz, Frederik and Hesser, J{\"u}rgen}, title = {Smart Radiation Therapy Biomaterials}, series = {International Journal of Radiation Oncology*Biology*Physics}, volume = {97}, journal = {International Journal of Radiation Oncology*Biology*Physics}, number = {3}, publisher = {Elsevier BV}, issn = {0360-3016}, doi = {https://doi.org/10.1016/j.ijrobp.2016.10.034}, pages = {624 -- 637}, year = {2017}, subject = {Krebs, Medizin}, language = {en} } @article{WiechSteinLachenmaieretal.2009, author = {Wiech, Thorsten and Stein, Stefan and Lachenmaier, Victoria and Schmitt, Eberhard and Schwarz-Finsterle, Jutta and Wiech, Elisabeth and Hildenbrand, Georg and Werner, Martin and Hausmann, Michael}, title = {Spatial allelic imbalance of BCL2 genes and chromosome 18 territories in nonneoplastic and neoplastic cervical squamous epithelium}, series = {European Biophysics Journal}, volume = {38}, journal = {European Biophysics Journal}, number = {6}, publisher = {Springer Science and Business Media LLC}, issn = {0175-7571}, doi = {https://doi.org/10.1007/s00249-009-0474-5}, pages = {793 -- 806}, year = {2009}, subject = {Genom}, language = {en} } @article{MuellerLemmermannKaufmannetal.2014, author = {M{\"u}ller, Patrick and Lemmermann, Niels A. and Kaufmann, Rainer and Gunkel, Manuel and Paech, Daniel and Hildenbrand, Georg and Holtappels, Rafaela and Cremer, Christoph and Hausmann, Michael}, title = {Spatial distribution and structural arrangement of a murine cytomegalovirus glycoprotein detected by SPDM localization microscopy}, series = {Histochemistry and Cell Biology}, volume = {142}, journal = {Histochemistry and Cell Biology}, number = {1}, publisher = {Springer Science and Business Media LLC}, issn = {0948-6143}, doi = {https://doi.org/10.1007/s00418-014-1185-2}, pages = {61 -- 67}, year = {2014}, subject = {Fluoreszenzmikroskopie}, language = {en} } @article{HildenbrandRappSpoerietal.2005, author = {Hildenbrand, Georg and Rapp, Alexander and Sp{\"o}ri, Udo and Wagner, Christian and Cremer, Christoph and Hausmann, Michael}, title = {Nano-Sizing of Specific Gene Domains in Intact Human Cell Nuclei by Spatially Modulated Illumination Light Microscopy}, series = {Biophysical Journal}, volume = {88}, journal = {Biophysical Journal}, number = {6}, publisher = {Elsevier BV}, issn = {0006-3495}, doi = {https://doi.org/10.1529/biophysj.104.056796}, pages = {4312 -- 4318}, year = {2005}, subject = {Genom}, language = {en} } @article{KaufmannMuellerHildenbrandetal.2010, author = {Kaufmann, Rainer and M{\"u}ller, P and Hildenbrand, Georg and Hausmann, Michael and Cremer, Christoph}, title = {Analysis of Her2/neu membrane protein clusters in different types of breast cancer cells using localization microscopy}, series = {Journal of Microscopy}, volume = {242}, journal = {Journal of Microscopy}, number = {1}, publisher = {Wiley}, issn = {0022-2720}, doi = {https://doi.org/10.1111/j.1365-2818.2010.03436.x}, pages = {46 -- 54}, year = {2010}, subject = {Krebs, Medizin}, language = {en} } @article{BurgerBiswasBarzanetal.2014, author = {Burger, Nina and Biswas, Abin and Barzan, Daniel and Kirchner, Anne and Hosser, Hiltraud and Hausmann, Michael and Hildenbrand, Georg and Herskind, Carsten and Wenz, Frederik and Veldwijk, Marlon R.}, title = {A method for the efficient cellular uptake and retention of small modified gold nanoparticles for the radiosensitization of cells}, series = {Nanomedicine: Nanotechnology, Biology and Medicine}, volume = {10}, journal = {Nanomedicine: Nanotechnology, Biology and Medicine}, number = {6}, publisher = {Elsevier BV}, issn = {1549-9634}, doi = {https://doi.org/10.1016/j.nano.2014.03.011}, pages = {1365 -- 1373}, year = {2014}, subject = {Gold}, language = {en} } @article{FalkHausmannLukasovaetal.2014, author = {Falk, Martin and Hausmann, Michael and Lukasova, Emilie and Biswas, Abin and Hildenbrand, Georg and Davidkova, Marie and Krasavin, Evgeny and Kleibl, Zdenek and Falkova, Iva and Jezkova, Lucie and Stefancikova, Lenka and Sevcik, Jan and Hofer, Michal and Bacikova, Alena and Matula, Pavel and Boreyko, Alla and Vachelova, Jana and Michaelidesova, Anna and Kozubek, Stanislav}, title = {Determining Omics Spatiotemporal Dimensions Using Exciting New Nanoscopy Techniques to Assess Complex Cell Responses to DNA Damage: PART A-Radiomics}, series = {Critical Reviews in Eukaryotic Gene Expression}, volume = {24}, journal = {Critical Reviews in Eukaryotic Gene Expression}, number = {3}, publisher = {Begell House}, issn = {1045-4403}, doi = {https://doi.org/10.1615/CritRevEukaryotGeneExpr.2014010313}, pages = {205 -- 223}, year = {2014}, subject = {Radiologie}, language = {en} } @incollection{HausmannPilarczykMausetal.2020, author = {Hausmann, Michael and Pilarczyk, G{\"o}tz and Maus, Emanuel and Hesser, J{\"u}rgen and Hildenbrand, Georg}, title = {Super-resolution microscopy of nanogold-labelling}, series = {Nanoparticle Enhanced Radiation Therapy}, booktitle = {Nanoparticle Enhanced Radiation Therapy}, publisher = {IOP Publishing}, isbn = {9780750323963}, doi = {https://doi.org/10.1088/978-0-7503-2396-3ch11}, pages = {11 -- 1}, year = {2020}, subject = {Krebs, Medizin}, language = {en} } @incollection{HausmannLeeHildenbrand2020, author = {Hausmann, Michael and Lee, Jin-Ho and Hildenbrand, Georg}, title = {3D DNA FISH for analyses of chromatin-nuclear architecture}, series = {Epigenetics Methods}, booktitle = {Epigenetics Methods}, publisher = {Elsevier}, isbn = {9780128194140}, doi = {https://doi.org/10.1016/B978-0-12-819414-0.00020-3}, pages = {399 -- 418}, year = {2020}, subject = {Genom}, language = {en} }