@article{PagačovaŠtefančikovaSchmidtKaleretal.2019, author = {Pag{\´a}čov{\´a}, Eva and Štefanč{\´i}kov{\´a}, Lenka and Schmidt-Kaler, Franz and Hildenbrand, Georg and Vičar, Tom{\´a}š and Depeš, Daniel and Lee, Jin-Ho and Bestvater, Felix and Lacombe, Sandrine and Porcel, Erika and Roux, St{\´e}phane and Wenz, Frederik and Kopecna, Olga and Falkov{\´a}, Iva and Hausmann, Michael and Falk, Martin}, title = {Challenges and Contradictions of Metal Nano-Particle Applications for Radio-Sensitivity Enhancement in Cancer Therapy}, series = {International Journal of Molecular Sciences}, volume = {20}, journal = {International Journal of Molecular Sciences}, number = {3}, publisher = {MDPI AG}, issn = {1422-0067}, doi = {https://doi.org/10.3390/ijms20030588}, year = {2019}, abstract = {From the very beginnings of radiotherapy, a crucial question persists with how to target the radiation effectiveness into the tumor while preserving surrounding tissues as undamaged as possible. One promising approach is to selectively pre-sensitize tumor cells by metallic nanoparticles. However, though the "physics" behind nanoparticle-mediated radio-interaction has been well elaborated, practical applications in medicine remain challenging and often disappointing because of limited knowledge on biological mechanisms leading to cell damage enhancement and eventually cell death. In the present study, we analyzed the influence of different nanoparticle materials (platinum (Pt), and gold (Au)), cancer cell types (HeLa, U87, and SKBr3), and doses (up to 4 Gy) of low-Linear Energy Transfer (LET) ionizing radiation (γ- and X-rays) on the extent, complexity and reparability of radiation-induced γH2AX + 53BP1 foci, the markers of double stand breaks (DSBs). Firstly, we sensitively compared the focus presence in nuclei during a long period of time post-irradiation (24 h) in spatially (three-dimensionally, 3D) fixed cells incubated and non-incubated with Pt nanoparticles by means of high-resolution immunofluorescence confocal microscopy. The data were compared with our preliminary results obtained for Au nanoparticles and recently published results for gadolinium (Gd) nanoparticles of approximately the same size (2-3 nm). Next, we introduced a novel super-resolution approach—single molecule localization microscopy (SMLM)—to study the internal structure of the repair foci. In these experiments, 10 nm Au nanoparticles were used that could be also visualized by SMLM. Altogether, the data show that different nanoparticles may or may not enhance radiation damage to DNA, so multi-parameter effects have to be considered to better interpret the radiosensitization. Based on these findings, we discussed on conclusions and contradictions related to the effectiveness and presumptive mechanisms of the cell radiosensitization by nanoparticles. We also demonstrate that SMLM offers new perspectives to study internal structures of repair foci with the goal to better evaluate potential differences in DNA damage patterns.}, subject = {Krebs, Medizin}, language = {en} } @article{FranzYangMicheletal.2023, author = {Franz, Daniel and Yang, Yongting and Michel, Luis and Esen, Cemal and Hellmann, Ralf}, title = {Evaluation of an ultrashort pulsed laser robot system for flexible and large-area micromachining}, series = {Journal of Laser Applications}, volume = {35}, journal = {Journal of Laser Applications}, number = {4}, pages = {042057 -- 042065}, year = {2023}, abstract = {We report for the first time on the realization, characterization and application of an ultrashort pulsed laser robot system for flexible and large-area 2D and 3D laser micromachining with 6 articulated axes. To characterize the dynamic positioning of the laser beam during and after axes movement, CMOS image sensors were integrated into the beam path. A method introduced for the alignment of the optical axes allows a reduction of the deviations in laser beam positioning to less than 141.8 ± 92.9 μm within a 110° rotation range of axis 4. In addition, a high laser beam positioning repeatability of less than 102.2 μm is demonstrated over a total period of 14 h for a movement of axis 5 within a range of 0° to 90°. Initial laser cutting, laser structuring and laser marking applications on automotive dashboards and glass substrates are presented for flexible and large area 2D and 3D manufacturing. By applying a special laser cutting strategy for processing AF 32 eco thin glass, high cutting quality is achieved with a taper of up to 96.3\% without the generation of cracks, demonstrating the innovative potential of the high-precision laser robot system. Nonetheless, different identified inherent influences of each axis 1-5 during robot axis movement demand for an innovative beam stabilization concept to achieve high precision in laser beam positioning.}, subject = {Ultrakurzzeitlaser}, language = {en} } @article{FranzSchusterSchwarzetal.2025, author = {Franz, Daniel and Schuster, David and Schwarz, Simon and Rung, Stefan and Esen, Cemal and Hellmann, Ralf}, title = {Fabrication and analysis of through-glass vias for glass-based electronic packaging using an ultrashort pulsed laser}, series = {Optics and Lasers in Engineering}, volume = {193}, journal = {Optics and Lasers in Engineering}, publisher = {Elsevier BV}, issn = {0143-8166}, doi = {10.1016/j.optlaseng.2025.109106}, year = {2025}, subject = {Ultrakurzzeitlaser}, language = {en} } @article{FranzYangEsenetal.2026, author = {Franz, Daniel and Yang, Yongting and Esen, Cemal and Hellmann, Ralf}, title = {Flexible 2D Laser Cutting of Ultra-Thin Glass Using an Ultrashort Pulsed Laser Robotic System}, series = {Journal of Laser Micro/Nanoengineering}, volume = {21}, journal = {Journal of Laser Micro/Nanoengineering}, number = {1}, editor = {Esen, Cemal}, publisher = {Japan Laser Processing Society}, issn = {1880-0688}, doi = {10.2961/jlmn.2026.01.2004}, year = {2026}, subject = {Laserschneiden}, language = {en} }