@article{Daus2012, author = {Daus, Andreas}, title = {Leben auf dem Mikrochip - Biosensoren auf der Basis von Mikroelektroden-Arrays}, series = {Labor \& More}, volume = {2012}, journal = {Labor \& More}, number = {8}, pages = {20 -- 24}, year = {2012}, subject = {Biosensor}, language = {de} } @phdthesis{Daus2013, author = {Daus, Andreas}, title = {Zellbasierte Biosensoren - Hybride Systeme aus dreidimensionalen in vitro Netzwerken und Mikroelektroden Arrays}, school = {Technische Hochschule Aschaffenburg}, year = {2013}, abstract = {Mit der Entwicklung hybrider Strukturen aus Mikroelektroden Arrays und dreidimensionalen Sph{\"a}roiden wurde in dieser Arbeit ein funktionaler, deskriptiver Ansatz der Biosensorik adressiert. W{\"a}hrend biochemische Sensoren ausschließlich analytische Informationen {\"u}ber die Wechselwirkung von Liganden und Rezeptoren liefern, erlauben zellbasierte Biosensoren eine physiologische Beschreibung biologischer Systeme in nativen bzw. chemisch oder physikalisch provozierten Zust{\"a}nden. Dies impliziert f{\"u}r Anwendungen der Pharmakologie, Toxikologie und Grundlagenforschung hohes Potenzial, evoziert jedoch eine multidisziplin{\"a}re Aufgabe. Es werden zum einen in vitro Systeme ben{\"o}tigt, die den Anspr{\"u}chen eines physiologisch repr{\"a}sentativen Abbilds eines Organismus gen{\"u}gen. Zum anderen sind technische Systeme erforderlich, die Zellreaktionen qualitativ und quantitativ verwertbar machen.}, subject = {Array}, language = {de} } @article{SamhaberSchottdorfElHadyetal.2016, author = {Samhaber, Robert and Schottdorf, Manuel and El Hady, Ahmed and Br{\"o}king, Kai and Daus, Andreas and Thielemann, Christiane and St{\"u}hmer, Walter and Wolf, Fred}, title = {Growing neuronal islands on multi-electrode arrays using an accurate positioning-μCP device}, series = {Journal of Neuroscience Methods}, volume = {2016}, journal = {Journal of Neuroscience Methods}, number = {257}, doi = {10.1016/j.jneumeth.2015.09.022}, pages = {194 -- 203}, year = {2016}, abstract = {Background: Multi-electrode arrays (MEAs) allow non-invasive multi-unit recording in-vitro from cultured neuronal networks. For sufficient neuronal growth and adhesion on such MEAs, substrate preparation is required. Plating of dissociated neurons on a uniformly prepared MEA's surface results in the formation of spatially extended random networks with substantial inter-sample variability. Such cultures are not optimally suited to study the relationship between defined structure and dynamics in neuronal networks. To overcome these shortcomings, neurons can be cultured with pre-defined topology by spatially structured surface modification. Spatially structuring a MEA surface accurately and reproducibly with the equipment of a typical cell-culture laboratory is challenging. New method: In this paper, we present a novel approach utilizing micro-contact printing (μCP) combined with a custom-made device to accurately position patterns on MEAs with high precision. We call this technique AP-μCP (accurate positioning micro-contact printing). Comparison with existing methods: Other approaches presented in the literature using μCP for patterning either relied on facilities or techniques not readily available in a standard cell culture laboratory, or they did not specify means of precise pattern positioning. Conclusion: Here we present a relatively simple device for reproducible and precise patterning in a standard cell-culture laboratory setting. The patterned neuronal islands on MEAs provide a basis for high throughput electrophysiology to study the dynamics of single neurons and neuronal networks.}, subject = {Mehrfachelektrode}, language = {en} } @article{NickDausBesteletal.2013, author = {Nick, Christoph and Daus, Andreas and Bestel, Robert and Goldhammer, Michael and Steger, Frederik and Thielemann, Christiane}, title = {DrCell - a software tool for the analysis of cell signals recorded with extracellular microelectrodes}, series = {Signal processing: an international journal (SPIJ)}, volume = {7}, journal = {Signal processing: an international journal (SPIJ)}, number = {2}, pages = {96 -- 109}, year = {2013}, subject = {Mikroelektrode}, language = {de} } @article{OsterDausErbesetal.2016, author = {Oster, Stefan and Daus, Andreas and Erbes, Christian and Goldhammer, Michael and Bochtler, Ulrich and Thielemann, Christiane}, title = {Long-term electromagnetic exposure of developing neuronal networks: A flexible experimental setup}, series = {Bioelectromagnetics}, volume = {2016}, journal = {Bioelectromagnetics}, number = {37}, doi = {10.1002/bem.21974}, pages = {264 -- 278}, year = {2016}, abstract = {Neuronal networks in vitro are considered one of the most promising targets of research to assess potential electromagnetic field induced effects on neuronal functionality. A few exposure studies revealed there is currently no evidence of any adverse health effects caused by weak electromagnetic fields. Nevertheless, some published results are inconsistent. Particularly, doubts have been raised regarding possible athermal biological effects in the young brain during neuronal development. Therefore, we developed and characterized a flexible experimental setup based on a transverse electromagnetic waveguide, allowing controlled, reproducible exposure of developing neuronal networks in vitro. Measurement of S-parameters confirmed very good performance of the Stripline in the band of 800-1000 MHz. Simulations suggested a flexible positioning of cell culture dishes throughout a large exposure area, as specific absorption rate values were quite independent of their position (361.7 ± 11.4 mW/kg) at 1 W, 900 MHz. During exposure, thermal drift inside cellular medium did not exceed 0.1 K. Embryonic rat cortical neurons were cultivated on microelectrode array chips to non-invasively assess electrophysiological properties of electrogenic networks. Measurements were taken for several weeks, which attest to the experimental setup being a reliable system for long-term studies on developing neuronal tissue.}, subject = {Neuronales Netz}, language = {en} } @inproceedings{FriessRitterDausetal.2013, author = {Frieß, Johannes and Ritter, Sylvia and Daus, Andreas and Layer, Paul and Thielemann, Christiane and Heselich, Anja}, title = {Electrophysiologic and Molecular Characteristics of Cardiomyocytes after Heavy Ion Irradiation in the frame of the ESA IBER-10 Program}, series = {Heavy Ion in Therapie and Space Radiation Symposium (HITSRS2013), 2013, Chiba, Japan}, booktitle = {Heavy Ion in Therapie and Space Radiation Symposium (HITSRS2013), 2013, Chiba, Japan}, year = {2013}, subject = {Herzmuskelzelle}, language = {en} } @inproceedings{DausOsterErbesetal.2013, author = {Daus, Andreas and Oster, Stefan and Erbes, Christian and Goldhammer, Michael and Bochtler, Ulrich and Thielemann, Christiane}, title = {Electromagnetic exposure of neuronal networks on microelectrode arrays}, series = {U.R.S.I. Landesausschuss in der Bundesrepublik Deutschland e.V., Kleinheubacher Tagung, Miltenberg, Abstracts}, booktitle = {U.R.S.I. Landesausschuss in der Bundesrepublik Deutschland e.V., Kleinheubacher Tagung, Miltenberg, Abstracts}, pages = {29 -- 30}, year = {2013}, subject = {Neuronales Netz}, language = {en} } @inproceedings{OsterDausGoldhammeretal.2013, author = {Oster, Stefan and Daus, Andreas and Goldhammer, Michael and Bochtler, Ulrich and Thielemann, Christiane}, title = {A Flexible RF Exposure Setup for Long-term Electrophysiological Investigations on Biological Samples in Vitro}, series = {Progress In Electromagnetics Research Symposium Abstracts, Stockholm, Schweden}, booktitle = {Progress In Electromagnetics Research Symposium Abstracts, Stockholm, Schweden}, year = {2013}, subject = {Neuronales Netz}, language = {en} } @article{BestelDausThielemann2012, author = {Bestel, Robert and Daus, Andreas and Thielemann, Christiane}, title = {A novel automated spike sorting algorithm with adaptable feature extraction}, series = {Journal of Neuroscience Methods}, volume = {211}, journal = {Journal of Neuroscience Methods}, number = {1}, doi = {10.1016/j.jneumeth.2012.08.015}, pages = {168 -- 178}, year = {2012}, subject = {Sortierverfahren}, language = {en} } @inproceedings{GoldhammerDausThielemannetal.2009, author = {Goldhammer, Michael and Daus, Andreas and Thielemann, Christiane and Bochtler, Ulrich}, title = {Flexible measurement setup for electromagnetic exposition of biological samples}, series = {BioEM 2009, Davos, Switzerland}, booktitle = {BioEM 2009, Davos, Switzerland}, year = {2009}, subject = {Elektromagnetische Strahlung}, language = {en} } @article{DausLayerThielemann2012, author = {Daus, Andreas and Layer, Paul and Thielemann, Christiane}, title = {A spheroid-based biosensor for the label-free detection of drug-induced field potential alterations}, series = {Sensors and actuators B: Chemical}, volume = {165}, journal = {Sensors and actuators B: Chemical}, number = {1}, doi = {10.1016/j.snb.2012.02.011}, pages = {53 -- 58}, year = {2012}, subject = {Biosensor}, language = {en} } @inproceedings{DausGoldhammerBochtleretal.2009, author = {Daus, Andreas and Goldhammer, Michael and Bochtler, Ulrich and Thielemann, Christiane}, title = {Electromagnetic exposition of neuronal networks in vitro}, series = {Radiofrequency fields and health}, booktitle = {Radiofrequency fields and health}, organization = {Forschungsgemeinschaft Funk, Stuttgart}, year = {2009}, subject = {Neuronales Netz}, language = {en} } @inproceedings{DausHasenkopfThielemann2009, author = {Daus, Andreas and Hasenkopf, Matthias and Thielemann, Christiane}, title = {Novel 3D cell culture systems for electromagnetic exposure studies}, series = {BioEM 2009, Davos, Switzerland}, booktitle = {BioEM 2009, Davos, Switzerland}, year = {2009}, subject = {Zellkultur}, language = {en} } @inproceedings{StegerFriessDausetal.2012, author = {Steger, Frederik and Frieß, Johannes and Daus, Andreas and Ritter, Sylvia and Thielemann, Christiane}, title = {Analysis of electrophysiological characteristics of cardiomyocytes following radiation exposure}, series = {8th International Meeting on Substrate-Integrated Microelectrode Arrays}, booktitle = {8th International Meeting on Substrate-Integrated Microelectrode Arrays}, issn = {2194-5519}, doi = {10.13140/RG.2.1.4153.0400}, pages = {153}, year = {2012}, subject = {Herzmuskelzelle}, language = {en} } @inproceedings{DausGoldhammerThielemann2010, author = {Daus, Andreas and Goldhammer, Michael and Thielemann, Christiane}, title = {Using three-dimensional cell culture systems on Microelectrode arrays for biosensing applications}, series = {7th international Meeting on subtrate-intergrated microelectrode arrays, Reutlingen}, booktitle = {7th international Meeting on subtrate-intergrated microelectrode arrays, Reutlingen}, isbn = {978-3-938345-08-5}, pages = {314 -- 315}, year = {2010}, subject = {Array}, language = {en} } @article{DausGoldhammerLayeretal.2011, author = {Daus, Andreas and Goldhammer, Michael and Layer, Paul and Thielemann, Christiane}, title = {Electromagnetic exposure of scaffold-free three-dimensional cell culture systems}, series = {Bioelectromagnetics}, volume = {32}, journal = {Bioelectromagnetics}, number = {5}, issn = {1521-186X}, doi = {10.1002/bem.20649}, pages = {351 -- 359}, year = {2011}, subject = {Zellkultur}, language = {en} } @inproceedings{ThielemannRitterLayeretal.2011, author = {Thielemann, Christiane and Ritter, Sylvia and Layer, Paul and Frieß, Johannes and Daus, Andreas and Steger, Frederik and Heselich, Anja}, title = {Cellular effects of space radiation with relevance to cardiovascular diseases}, series = {Life in Space for Life on Earth}, booktitle = {Life in Space for Life on Earth}, pages = {560}, year = {2011}, subject = {Kardiovaskul{\"a}re Krankheit}, language = {en} } @inproceedings{DausBestelThielemann2011, author = {Daus, Andreas and Bestel, Robert and Thielemann, Christiane}, title = {Spike Detection, Refinement And Interpretation Of Recordings In Three-Dimensional In Vitro Systems}, series = {World Congress of Regenerative Medicine \& Stem Cells 2011, Beijing, China}, booktitle = {World Congress of Regenerative Medicine \& Stem Cells 2011, Beijing, China}, year = {2011}, subject = {Neuronales Netz}, language = {en} } @inproceedings{DausGoldhammerBochtleretal.2010, author = {Daus, Andreas and Goldhammer, Michael and Bochtler, Ulrich and Thielemann, Christiane}, title = {A new approach for the investigation of long-term effects of RF radiation on cells}, series = {28th Progress In Electromagnetics Research Symposium 2010, Cambridge}, booktitle = {28th Progress In Electromagnetics Research Symposium 2010, Cambridge}, year = {2010}, subject = {Hochfrequenz}, language = {en} } @article{DausThielemann2010, author = {Daus, Andreas and Thielemann, Christiane}, title = {Spheroids on microelectrode arrays: A highly sensitive system for biosensing applications}, series = {European Journal of cell biology}, journal = {European Journal of cell biology}, number = {89 Sonderheft 1}, pages = {67 -- 68}, year = {2010}, subject = {Array}, language = {en} }