@article{SchaeferHildenbrandHausmann2024, author = {Sch{\"a}fer, Myriam and Hildenbrand, Georg and Hausmann, Michael}, title = {Impact of Gold Nanoparticles and Ionizing Radiation on Whole Chromatin Organization as Detected by Single-Molecule Localization Microscopy}, series = {International Journal of Molecular Sciences}, volume = {25}, journal = {International Journal of Molecular Sciences}, number = {23}, publisher = {MDPI AG}, issn = {1422-0067}, doi = {https://doi.org/10.3390/ijms252312843}, year = {2024}, abstract = {In radiation tumor therapy, irradiation, on one hand, should cause cell death to the tumor. On the other hand, the surrounding non-tumor tissue should be maintained unaffected. Therefore, methods of local dose enhancements are highly interesting. Gold nanoparticles, which are preferentially uptaken by very-fast-proliferating tumor cells, may enhance damaging. However, the results in the literature obtained from cell culture and animal tissue experiments are very contradictory, i.e., only some experiments reveal increased cell killing but others do not. Thus, a better understanding of cellular mechanisms is required. Using the breast cancer cell model SkBr3, the effects of gold nanoparticles in combination with ionizing radiation on chromatin network organization were investigated by Single-Molecule Localization Microscopy (SMLM) and applications of mathematical topology calculations (e.g., Persistent Homology, Principal Component Analysis, etc.). The data reveal a dose and nanoparticle dependent re-organization of chromatin, although colony forming assays do not show a significant reduction of cell survival after the application of gold nanoparticles to the cells. In addition, the spatial organization of γH2AX clusters was elucidated, and characteristic changes were obtained depending on dose and gold nanoparticle application. The results indicate a complex response of ALU-related chromatin and heterochromatin organization correlating to ionizing radiation and gold nanoparticle incorporation. Such complex whole chromatin re-organization is usually associated with changes in genome function and supports the hypothesis that, with the application of gold nanoparticles, not only is DNA damage increasing but also the efficiency of DNA repair may be increased. The understanding of complex chromatin responses might help to improve the gold nanoparticle efficiency in radiation treatment.}, subject = {Krebs, Medizin}, language = {en} } @article{FaillaAlbrechtSpoerietal.2003, author = {Failla, Antonio Virgillo and Albrecht, Benno and Sp{\"o}ri, U. and Schweitzer, A. and Kroll, A. and Hildenbrand, Georg and Bach, M. and Cremer, Christoph}, title = {Nanostructure Analysis Using Spatially Modulated Illumination Microscopy}, series = {Complexus}, volume = {1}, journal = {Complexus}, number = {2}, publisher = {S. Karger AG}, issn = {1424-8492}, doi = {https://doi.org/10.1159/000070464}, pages = {77 -- 88}, year = {2003}, abstract = {For an improved understanding of cellular processes, it is highly desirable to develop light optical methods for the analysis of biological nanostructures and their dynamics in the interior of three-dimensionally (3D) conserved cells. Here, important structural parameters to be considered are the topology, i.e. the mutual positions and distances, as well as the sizes of the constituting subunits. This has become possible by the development of a novel method of far-field light fluorescence microscopy, spatially modulated illumination (SMI) microscopy. Using this approach, axial distances between fluorescence-labeled targets can be measured with an accuracy close to 1 nm; their sizes can be determined down to a few tens of nanometers. This approach can be extended to the determination of 3D positions and mutual 3D distances and sizes of any number of small objects/subunits that can be discriminated due to their spectral signatures. Consequently, the new approach allows an 'in situ nanostructure elucidation, until now regarded to be beyond the possibilities of far-field light microscopy. Application examples discussed are: colocalization/nanosizing and topological analysis of large protein-protein complexes, of nucleic acid-protein complexes (such as transcription factories), or of the highly complex DNA-protein nanostructures of which active/ inactive gene regions in the eukaryotic cell nucleus are constituted.}, subject = {Fluoreszenzmikroskopie}, language = {en} } @article{HildenbrandRappSpoerietal.2005, author = {Hildenbrand, Georg and Rapp, Alexander and Sp{\"o}ri, Udo and Wagner, Christian and Cremer, Christoph and Hausmann, Michael}, title = {Nano-Sizing of Specific Gene Domains in Intact Human Cell Nuclei by Spatially Modulated Illumination Light Microscopy}, series = {Biophysical Journal}, volume = {88}, journal = {Biophysical Journal}, number = {6}, publisher = {Elsevier BV}, issn = {0006-3495}, doi = {https://doi.org/10.1529/biophysj.104.056796}, pages = {4312 -- 4318}, year = {2005}, subject = {Genom}, language = {en} } @article{WagnerHildenbrandSpoerietal.2006, author = {Wagner, Christian and Hildenbrand, Georg and Sp{\"o}ri, Udo and Cremer, Christoph}, title = {Beyond nanosizing: an approach to shape analysis of fluorescent nanostructures by SMI-microscopy}, series = {Optik}, volume = {117}, journal = {Optik}, number = {1}, publisher = {Elsevier BV}, issn = {0030-4026}, doi = {https://doi.org/10.1016/j.ijleo.2005.05.006}, pages = {26 -- 32}, year = {2006}, subject = {Fluoreszenzmikroskopie}, language = {en} } @article{WiechSteinLachenmaieretal.2009, author = {Wiech, Thorsten and Stein, Stefan and Lachenmaier, Victoria and Schmitt, Eberhard and Schwarz-Finsterle, Jutta and Wiech, Elisabeth and Hildenbrand, Georg and Werner, Martin and Hausmann, Michael}, title = {Spatial allelic imbalance of BCL2 genes and chromosome 18 territories in nonneoplastic and neoplastic cervical squamous epithelium}, series = {European Biophysics Journal}, volume = {38}, journal = {European Biophysics Journal}, number = {6}, publisher = {Springer Science and Business Media LLC}, issn = {0175-7571}, doi = {https://doi.org/10.1007/s00249-009-0474-5}, pages = {793 -- 806}, year = {2009}, subject = {Genom}, language = {en} } @article{KaufmannMuellerHildenbrandetal.2010, author = {Kaufmann, Rainer and M{\"u}ller, P and Hildenbrand, Georg and Hausmann, Michael and Cremer, Christoph}, title = {Analysis of Her2/neu membrane protein clusters in different types of breast cancer cells using localization microscopy}, series = {Journal of Microscopy}, volume = {242}, journal = {Journal of Microscopy}, number = {1}, publisher = {Wiley}, issn = {0022-2720}, doi = {https://doi.org/10.1111/j.1365-2818.2010.03436.x}, pages = {46 -- 54}, year = {2010}, subject = {Krebs, Medizin}, language = {en} } @article{MuellerLemmermannKaufmannetal.2014, author = {M{\"u}ller, Patrick and Lemmermann, Niels A. and Kaufmann, Rainer and Gunkel, Manuel and Paech, Daniel and Hildenbrand, Georg and Holtappels, Rafaela and Cremer, Christoph and Hausmann, Michael}, title = {Spatial distribution and structural arrangement of a murine cytomegalovirus glycoprotein detected by SPDM localization microscopy}, series = {Histochemistry and Cell Biology}, volume = {142}, journal = {Histochemistry and Cell Biology}, number = {1}, publisher = {Springer Science and Business Media LLC}, issn = {0948-6143}, doi = {https://doi.org/10.1007/s00418-014-1185-2}, pages = {61 -- 67}, year = {2014}, subject = {Fluoreszenzmikroskopie}, language = {en} } @article{FalkHausmannLukasovaetal.2014, author = {Falk, Martin and Hausmann, Michael and Lukasova, Emilie and Biswas, Abin and Hildenbrand, Georg and Davidkova, Marie and Krasavin, Evgeny and Kleibl, Zdenek and Falkova, Iva and Jezkova, Lucie and Stefancikova, Lenka and Sevcik, Jan and Hofer, Michal and Bacikova, Alena and Matula, Pavel and Boreyko, Alla and Vachelova, Jana and Michaelidesova, Anna and Kozubek, Stanislav}, title = {Determining Omics Spatiotemporal Dimensions Using Exciting New Nanoscopy Techniques to Assess Complex Cell Responses to DNA Damage: Part - Structuromics}, series = {Critical Reviews in Eukaryotic Gene Expression}, volume = {24}, journal = {Critical Reviews in Eukaryotic Gene Expression}, number = {3}, publisher = {Begell House}, issn = {1045-4403}, doi = {https://doi.org/10.1615/CritRevEukaryotGeneExpr.v24.i3.40}, pages = {225 -- 247}, year = {2014}, subject = {Radiologie}, language = {en} } @article{FalkHausmannLukasovaetal.2014, author = {Falk, Martin and Hausmann, Michael and Lukasova, Emilie and Biswas, Abin and Hildenbrand, Georg and Davidkova, Marie and Krasavin, Evgeny and Kleibl, Zdenek and Falkova, Iva and Jezkova, Lucie and Stefancikova, Lenka and Sevcik, Jan and Hofer, Michal and Bacikova, Alena and Matula, Pavel and Boreyko, Alla and Vachelova, Jana and Michaelidesova, Anna and Kozubek, Stanislav}, title = {Determining Omics Spatiotemporal Dimensions Using Exciting New Nanoscopy Techniques to Assess Complex Cell Responses to DNA Damage: PART A-Radiomics}, series = {Critical Reviews in Eukaryotic Gene Expression}, volume = {24}, journal = {Critical Reviews in Eukaryotic Gene Expression}, number = {3}, publisher = {Begell House}, issn = {1045-4403}, doi = {https://doi.org/10.1615/CritRevEukaryotGeneExpr.2014010313}, pages = {205 -- 223}, year = {2014}, subject = {Radiologie}, language = {en} } @article{BurgerBiswasBarzanetal.2014, author = {Burger, Nina and Biswas, Abin and Barzan, Daniel and Kirchner, Anne and Hosser, Hiltraud and Hausmann, Michael and Hildenbrand, Georg and Herskind, Carsten and Wenz, Frederik and Veldwijk, Marlon R.}, title = {A method for the efficient cellular uptake and retention of small modified gold nanoparticles for the radiosensitization of cells}, series = {Nanomedicine: Nanotechnology, Biology and Medicine}, volume = {10}, journal = {Nanomedicine: Nanotechnology, Biology and Medicine}, number = {6}, publisher = {Elsevier BV}, issn = {1549-9634}, doi = {https://doi.org/10.1016/j.nano.2014.03.011}, pages = {1365 -- 1373}, year = {2014}, subject = {Gold}, language = {en} }