TY - JOUR A1 - Mayer, Margot A1 - Arrizabalaga, Onetsine A1 - Lieb, Florian A1 - Ciba, Manuel A1 - Ritter, Sylvia A1 - Thielemann, Christiane T1 - Electrophysiological investigation of human embryonic stem cell derived neurospheres using a novel spike detection algorithm T2 - Biosensors and Bioelectronics N2 - Microelectrode array (MEA) technology in combination with three-dimensional (3D) neuronal cell models derived from human embryonic stem cells (hESC) provide an excellent tool for neurotoxicity screening. Yet, there are significant challenges in terms of data processing and analysis, since neuronal signals have very small amplitudes and the 3D structure enhances the level of background noise. Thus, neuronal signal analysis requires the application of highly sophisticated algorithms. In this study, we present a new approach optimized for the detection of spikes recorded from 3D neurospheres (NS) with a very low signal-to-noise ratio. This was achieved by extending simple threshold-based spike detection utilizing a highly sensitive algorithm named SWTTEO. This analysis procedure was applied to data obtained from hESC-derived NS grown on MEA chips. Specifically, we examined changes in the activity pattern occurring within the first ten days of electrical activity. We further analyzed the response of NS to the GABA receptor antagonist bicuculline. With this new algorithm method we obtained more reliable results compared to the simple threshold-based spike detection. KW - Microelectrode array KW - Neurosphere KW - Spike detection algorithm KW - Human embryonic stem cell-derived neurons KW - SWTTEO KW - Embryonale Stammzelle Y1 - 2017 UR - https://opus4.kobv.de/opus4-h-ab/frontdoor/index/index/docId/1517 VL - 2018 IS - 100 SP - 462 EP - 468 ER -