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Fast protein and metabolite liquid chromatography (FPLMC) was introduced years ago to enable the easy separation of high-molecular compounds such as proteins from small molecules and the identification of the low-molecular substances. In this paper, the method is applied for the rapid evaluation of freshness and monitoring the aging of animal meat and fish. A novel chromatographic sensor was developed with a deep UV LED-based photometric detection unit (255–265 nm), an original flow cuvette and registration scheme; the processing of a chromatogram with the sensor takes approximately 15 min. Strict isochronism between the elution of ATP metabolites, mainly hypoxanthine (Hx) and inosine monophosphate (IMP), and the time of maturation of meat or fish, was discovered. A new freshness index H* = [Hx]/[IMP] was introduced, which is proportional to the instrumental delay time in the FPMLC chromatograms: the H* index < 0.5 indicates the presence of inosine monophosphate (IMP) and the high quality of the meat or fish. Reasonably strong correlations were revealed between data obtained by FPMLC and total volatile basic nitrogen TVB-N (for fish) or volatile fatty acids VFA (for meat) content. Moreover, putative nucleotide salvage and an increase in the concentration of IMP were observed in fish after heat treatment using the FPMLC sensor and NMR technique.
A sample set of calculations is presented in this supplemental document to analytically estimate joint distortion for gas metal arc lap joining of aluminum and steel thin sheets following the published article "Analytical Estimation of Thermomechanical Distortion and Interface Layer Thickness for Gas Metal Arc Lap Joining of Dissimilar Sheets"
Untersuchung der Rotorfestigkeit von permanenterregten Synchronmaschinen mit vergrabenen Magneten
(2023)
Diese Projektarbeit behandelt die mechanische Simulation der Rotoren von permanenterregten Synchronmaschinen für den Festigkeitsnachweis. Hierzu wird die Finite-Elemente-Methode mit dem Programm CalculiX verwendet. Als Beispiel wird der Elektromotor des Toyota Prius II im Detail betrachtet. Hierbei werden die unterschiedlichen Lasten modelliert, dazu zählen neben der Zentrifugalkraft auch der Innendruck durch eine Presspassung sowie die elektromagnetischen Kräfte. Für die Modellierung von Kräften auf der Rotoroberfläche wird eine Methodik zur Umsetzung in CalculiX entwickelt und anhand eines Beispielmodells demonstriert. Der angestrebte Festigkeitsnachweis kann im Rahmen dieser Arbeit erbracht werden. Weiterhin wird gezeigt, dass der Einfluss der elektromagnetischen Kräfte im Arbeitspunkt mit der höchsten Drehzahl sehr gering ausfällt. Insgesamt bietet diese Arbeit die Grundlagen für mechanische Modellierungen von Rotoren unter Berücksichtigung aller Kräfte. Dies kann direkt auf andere Elektromotoren angewendet werden.
Single dielectric microspheres can manipulate light focusing and collection to enhance optical interaction with surfaces. To demonstrate this principle, we experimentally investigate the enhancement of the Raman signal collected by a single dielectric microsphere, with a radius much larger than the exciting laser spot size, residing on the sample surface. The absolute microsphere-assisted Raman signal from a single graphene layer measured in air is more than a factor of two higher than that obtained with a high numerical aperture objective. Results from Mie’s theory are used to benchmark numerical simulations and an analytical model to describe the isolated microsphere focusing properties. The analytical model and the numerical simulations justify the Raman signal enhancement measured in the microsphere-assisted Raman spectroscopy experiments.
Biomass gasification is recognized as a viable avenue to accelerate the sustainable production of hydrogen. In this work, a numerical simulation model of air gasification of rice husks is developed using the Aspen Plus to investigate the feasibility of producing hydrogen-rich syngas. The model is experimentally validated with rice husk gasification results and other published studies. The influence of temperature and equivalence ratio on the syngas composition, H2 yield, LHVSyngas, H2/CO ratio, CGE, and PCG was studied. Furthermore, the synchronized effects of temperature and ER are studied using RSM to determine the operational point of maximizing H2 yield and PCG. The RSM analysis results show optimum performance at temperatures between 820 °C and 1090 °C and ER in the range of 0.06–0.10. The findings show that optimal operating conditions of the gasification system can be achieved at a more refined precision through simulations coupled with advanced optimization techniques.
Glass-ceramic composites consisting of potassium-iron-silicate glass and barium titanate mixed in various proportions were successfully synthesized by low-temperature sintering. The crystal structure of the obtained composite samples, the porosity and the microhardness were studied by the X-ray diffraction, the electron microscopy, the weight method, and the Vickers method. Electrical characteristics (dielectric permittivity, tunability and losses) of as-prepared and annealed in oxygen medium samples were investigated at microwaves. According to structural analysis, the synthesized samples are a mixture of KFeSi glass, ferroelectric BaTiO3, and dielectric barium polytitanates; the ratio of the latter determines the electrical properties of the composites. Depending on the content of barium titanate, the studied composite samples show a permittivity from 50 to 270 with a dielectric loss level of 0,1–0,02 in frequency range from 3 to 10 GHz. Annealing of composite samples in an oxygen-containing environment leads to an increase in their dielectric permittivity and tunability by 10–25% and a twofold decrease in dielectric loss.
Making and interpretation of drawings and schematics is an important skill to be developed in engineering education. The question type STACK in the Moodle learning management system combined with the interactive graphics library JSXGraph has a great potential for implementation of e-learning resources addressing these skills in formative and summative settings. The authoring process of such materials is complex due to multiple markup and programming languages like HTML, LaTeX, Maxima and JavaScript (JS) being involved. The Meclib concept mitigates this complexity and allows for efficient bulk production of material with consistent appearance and user experience. The core of Meclib is a set of pre-defined JSXGraph-based objects with an interface to Maxima, such that no problem specific JS code is required at all. The present paper outlines the basic ideas of the implementation and demonstrates the approach for some typical examples of different complexity. The focus is on applications in engineering mechanics, starting from static illustrations up to an editor for free body diagrams, with rich adaptive formative feedback.