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Fast protein and metabolite liquid chromatography (FPLMC) was introduced years ago to enable the easy separation of high-molecular compounds such as proteins from small molecules and the identification of the low-molecular substances. In this paper, the method is applied for the rapid evaluation of freshness and monitoring the aging of animal meat and fish. A novel chromatographic sensor was developed with a deep UV LED-based photometric detection unit (255–265 nm), an original flow cuvette and registration scheme; the processing of a chromatogram with the sensor takes approximately 15 min. Strict isochronism between the elution of ATP metabolites, mainly hypoxanthine (Hx) and inosine monophosphate (IMP), and the time of maturation of meat or fish, was discovered. A new freshness index H* = [Hx]/[IMP] was introduced, which is proportional to the instrumental delay time in the FPMLC chromatograms: the H* index < 0.5 indicates the presence of inosine monophosphate (IMP) and the high quality of the meat or fish. Reasonably strong correlations were revealed between data obtained by FPMLC and total volatile basic nitrogen TVB-N (for fish) or volatile fatty acids VFA (for meat) content. Moreover, putative nucleotide salvage and an increase in the concentration of IMP were observed in fish after heat treatment using the FPMLC sensor and NMR technique.
Single dielectric microspheres can manipulate light focusing and collection to enhance optical interaction with surfaces. To demonstrate this principle, we experimentally investigate the enhancement of the Raman signal collected by a single dielectric microsphere, with a radius much larger than the exciting laser spot size, residing on the sample surface. The absolute microsphere-assisted Raman signal from a single graphene layer measured in air is more than a factor of two higher than that obtained with a high numerical aperture objective. Results from Mie’s theory are used to benchmark numerical simulations and an analytical model to describe the isolated microsphere focusing properties. The analytical model and the numerical simulations justify the Raman signal enhancement measured in the microsphere-assisted Raman spectroscopy experiments.
Glass-ceramic composites consisting of potassium-iron-silicate glass and barium titanate mixed in various proportions were successfully synthesized by low-temperature sintering. The crystal structure of the obtained composite samples, the porosity and the microhardness were studied by the X-ray diffraction, the electron microscopy, the weight method, and the Vickers method. Electrical characteristics (dielectric permittivity, tunability and losses) of as-prepared and annealed in oxygen medium samples were investigated at microwaves. According to structural analysis, the synthesized samples are a mixture of KFeSi glass, ferroelectric BaTiO3, and dielectric barium polytitanates; the ratio of the latter determines the electrical properties of the composites. Depending on the content of barium titanate, the studied composite samples show a permittivity from 50 to 270 with a dielectric loss level of 0,1–0,02 in frequency range from 3 to 10 GHz. Annealing of composite samples in an oxygen-containing environment leads to an increase in their dielectric permittivity and tunability by 10–25% and a twofold decrease in dielectric loss.