TY - JOUR A1 - Kuznetsov, Arthur A1 - Frorip, Aleksandr A1 - Sünter, Alar A1 - Kasvand, Nensi A1 - Korsakov, Vadim A1 - Konoplev, Georgii A1 - Stepanova, Oksana A1 - Rusalepp, Linda A1 - Anton, Dea A1 - Püssa, Tõnu A1 - Roasto, Mati A1 - Abramova, Liubov A1 - Kozin, Andrey A1 - Toom, Lauri A1 - Hirsch, Sören A1 - Mukhin, Nikolay ED - mdpi, T1 - Fast Protein and Metabolites (Nucleotides and Nucleosides) Liquid Chromatography Technique and Chemical Sensor for the Assessment of Fish and Meat Freshness JF - Chemosensors N2 - Fast protein and metabolite liquid chromatography (FPLMC) was introduced years ago to enable the easy separation of high-molecular compounds such as proteins from small molecules and the identification of the low-molecular substances. In this paper, the method is applied for the rapid evaluation of freshness and monitoring the aging of animal meat and fish. A novel chromatographic sensor was developed with a deep UV LED-based photometric detection unit (255–265 nm), an original flow cuvette and registration scheme; the processing of a chromatogram with the sensor takes approximately 15 min. Strict isochronism between the elution of ATP metabolites, mainly hypoxanthine (Hx) and inosine monophosphate (IMP), and the time of maturation of meat or fish, was discovered. A new freshness index H* = [Hx]/[IMP] was introduced, which is proportional to the instrumental delay time in the FPMLC chromatograms: the H* index < 0.5 indicates the presence of inosine monophosphate (IMP) and the high quality of the meat or fish. Reasonably strong correlations were revealed between data obtained by FPMLC and total volatile basic nitrogen TVB-N (for fish) or volatile fatty acids VFA (for meat) content. Moreover, putative nucleotide salvage and an increase in the concentration of IMP were observed in fish after heat treatment using the FPMLC sensor and NMR technique. Y1 - 2023 UR - https://www.mdpi.com/2227-9040/11/1/69 U6 - https://doi.org/10.3390/chemosensors11010069 SN - 2227-9040 IS - 1/2023 / Band 11 PB - MDPI ER - TY - JOUR A1 - Kuznetsov, Artur A1 - Frorip, Aleksandr A1 - Sünter, Alar A1 - Korsakov, Vadim A1 - Konoplev, Georgii A1 - Stepanova, Oksana A1 - Roschina, Natalia A1 - Ovsyannikov, Nikolay A1 - Lialin, Daniil A1 - Gerasimchuk, Roman A1 - Dmitriev, Alexander A1 - Mukhin, Nikolay A1 - Hirsch, Sören T1 - Optical Chemical Sensor Based on Fast-Protein Liquid Chromatography for Regular Peritoneal Protein Loss Assessment in End-Stage Renal Disease Patients on Continuous Ambulatory Peritoneal Dialysis JF - Chemosensors KW - optical chemical sensor KW - fast-protein liquid chromatography KW - UV LED KW - end-stage renal disease KW - peritoneal dialysis KW - peritoneal protein loss KW - albumin KW - point-of-care diagnostic devices Y1 - 2022 U6 - https://doi.org/10.3390/chemosensors10060232 VL - 10 IS - 6 PB - MDPI ER - TY - JOUR A1 - Konoplev, Georgii A1 - Agafonova, Darina A1 - Bakhchova, Liubov A1 - Mukhin, Nikolay A1 - Kurachkina, Marharyta A1 - Schmidt, Marc-Peter A1 - Verlov, Nikolay A1 - Sidorov, Alexander A1 - Oseev, Aleksandr A1 - Stepanova, Oksana A1 - Kozyrev, Andrey A1 - Dmitriev, Alexander A1 - Hirsch, Sören T1 - Label-Free Physical Techniques and Methodologies for Proteins Detection in Microfluidic Biosensor Structures JF - Biomedicines KW - label-free biosensor KW - microfluidics KW - proteins detection KW - optical biosensors KW - impedance spectroscopy KW - plasmon resonance KW - point-of-care KW - diagnostic devices Y1 - 2022 U6 - https://doi.org/10.3390/biomedicines10020207 SN - 2227-9059 VL - 10 IS - 2 SP - 207 PB - MDPI ER - TY - JOUR A1 - Mukhin, Nikolay A1 - Konoplev, Georgii A1 - Oseev, Aleksandr A1 - Schmidt, Marc-Peter A1 - Stepanova, Oksana A1 - Kozyrev, Andrey A1 - Dmitriev, Alexander A1 - Hirsch, Soeren T1 - Label-Free Protein Detection by Micro-Acoustic Biosensor Coupled with Electrical Field Sorting. Theoretical Study in Urine Models JF - Sensors KW - acoustic liquid sensor KW - shear bulk acoustic resonator KW - biosensor KW - structured sensor interface KW - electrical field manipulation KW - urine proteins characterisation Y1 - 2021 U6 - https://doi.org/10.3390/s21072555 VL - 21 IS - 7 SP - 2555 PB - MDPI ER -