TY - JOUR A1 - Beck, Eberhard A1 - Moldenhauer, A. A1 - Merkle, E. A1 - Kiesewetter, F. A1 - Jäger, W. A1 - Wildt, L. A1 - Lang, N. T1 - CA 125 production an release by ovarian cancer cell lines in vitro JF - In: The international journal of biological markers 13(1998), 200 – 206 Y1 - 1998 SP - 200 EP - 206 ER - TY - JOUR A1 - Beck, Eberhard A1 - Hofmann, M. A1 - Bernhardt, G. A1 - Jäger, W. A1 - Wildt, L. A1 - Lang, N. T1 - In vitro activity of immunoconjugates between cisplatin and an anti CA125 monoclonal antibody on ovarian cancer cell lines JF - In: Cell Biophysics 24/25(1994)1-3, 163-173 N2 - Cis-diammine dichloro platinum (II) (CDDP), is a highly potent antineoplastic agent that is used in the treatment of ovarian cancer. However, the clinical use of CDDP is restricted by its severe side effects. In order to reduce these side effects and to enhance its therapeutic efficacy, we developed specific immunoconjugates consisting of the murine monoclonal antibody OC125 and CDDP, using diethylene triamine pentaacetic acid (DTPA) as a linker. The coupling efficiencies of the different preparations synthesized, varied between 1.10±0.42 and 2.65±1.60 mol of CDDP per mol of antibody protein. Despite the chemical modification of the antibody molecule, specific binding activity of the OC125-CDDP conjugates toward the CA125 antigen was maintained as was demonstrated by means of immunohisto-/cytochemical staining of frozen sections of ovarian cancer tissue, amniotic epithelium, and the CA125 positive ovarian cancer cell line NIH:OVCAR 3. The antiproliferative activity of the immunoconjugates was tested against the human ovarian cancer cell lines NIH:OVCAR3 and SKOV 3, applying a kinetic crystal violet microassay. Despite the promising results obtained with the specific immuno-staining of the target cells, no significant antiproliferative activity of our immunoconjugates against the cell lines tested was observed. One possible explanation for the lack of antitumor activity could be the fact that CA125 is released in large amounts by the NIH:OVCAR 3 cells. This may have prevented an efficient immunotargeting of the cancer cells by the formation of soluble immune complexes. Y1 - 1994 SN - 0163-4992 SP - 163 EP - 173 ER - TY - JOUR A1 - Beck, Eberhard A1 - Russo, P. A1 - Papa, V. A1 - Jäger, W. A1 - Pezzino, V. A1 - Wildt, L. A1 - Vigneri, R. A1 - Lang, N. T1 - Nachweis von Insulin-Rezeptoren (IR) in Ovarialkarzinomgewebe JF - In: Archives of Gynecology and Obstetrics December 254(1993)Iss. 1-4, 1053-1054 Y1 - 1993 SN - 0932-0067 SP - 1053 EP - 1054 ER - TY - CHAP A1 - Bittl, A. A1 - Beck, Eberhard A1 - Jäger, W. A1 - Wildt, L. T1 - Untersuchungen zur Amenorrhoe unter adjuvanter CMF-Chemotherapie bei Mammakarzinom-Patientinnen T2 - In: Zytostatikabedingte Nebenwirkungen : Symposium in Pommersfelden, 3. - 4. Juli 1992; 26 Tabellen / Bd.-Hrsg.: N. Lang; W. Jäger. - München [u.a.] : Zuckschwerdt, 1992. - (Aktuelle Onkologie ; 68). - ISBN: 3-88603-458-5 Y1 - 1993 SP - 10 EP - 14 ER - TY - CHAP A1 - Beck, Eberhard A1 - Hofmann, M. A1 - Kiesewetter, F. A1 - Jäger, W. A1 - Wildt, L. A1 - Lang, N. T1 - Analysis of CA 125 release by ovarian cancer cells in vitro T2 - In: Tumor associated antigens, oncogenes, receptors, cytokines in tumor diagnosis and therapy at the beginning of the nineties / 6th Symposium on Tumor Markers, Hamburg, [December 9. - 11. 1991] ; Satellite Symposium Cancer of the Breast - State and Trends in Diagnosis and Therapy. Ed. by R. - München [u.a.] : Zuckschwerdt, 1992. - ISBN 3-88603-444-5 Y1 - 1992 SP - 117 EP - 121 ER - TY - CHAP A1 - Beck, Eberhard A1 - Seidinger, M. A1 - Schneider, G. A. A1 - Schuster, M. A1 - Berg, A. A1 - Jäger, W. A1 - Wildt, L. A1 - Lang, N. T1 - Toxische Schädigungen des Gehörs unter Cisplatinhaltiger Chemotherapie bei Patientinnen mit gynäkologischen Tumoren T2 - In: Zytostatikabedingte Nebenwirkungen : Symposium in Pommersfelden, 3. - 4. Juli 1992 ; 26 Tabellen / Bd-Hrsg.: N. Lang ... - München [u.a.] : Zuckschwerdt, 1993. - Aktuelle Onkologie ; 8). - ISBN: 3-88603-458-5 Y1 - 1992 SP - 5 EP - 9 ER - TY - CHAP A1 - Jäger, W. A1 - Löffler, B. A1 - Beck, Eberhard A1 - Bellmann, O. A1 - Wolf, A. A1 - Mund-Hoym, S. A1 - Sauerbrei, W. A1 - Wildt, L. A1 - Lang, N. T1 - Retrospective analysis of GnRH analogue administration in patients with advanced ovarian cancer T2 - In: GnRH analogues and cancer : plus a review of the treatment of uterine leiomyomata; the proceedings of the 2. International Symposium on GnRH Analogues in Cancer and Human Reproduction, Geneva, November 1990 / ed. by B. Lunenfeld. - Carnforth, Lancs. [u.a.] : Parthenon Publ., 1992. - (Advances in the study of GnRH analogues ; 4). - ISBN: 1-85070-328-0 Y1 - 1992 SP - 69 EP - 76 ER -