TY - CHAP A1 - Beck, Eberhard A1 - Sciacca, Laura A1 - Pandini, Giuseppe A1 - Jaeger, Wolfram A1 - Pezzino, Vincenzo T1 - Measurement of IGF-1 Receptor Content in Tissues and Cell Lines by Radioimmunoassay (RIA) and ELISA Techniques T2 - In: Ovarian Cancer : Methods and Protocols / ed. by John M. S. Bartlett. - Totowa, NJ : Humana Press, 2001. - Totowa, NJ : Humana Press, 2001. - (Methods in Molecular Medicineā„¢ ; 39). - ISBN: 978-1-592-59071-1; 978-0-89603-583-6 (ISBN der Printausgabe) N2 - The IGF-1 receptor (IGF-1-R) belongs to the tyrosine kinase growth factor receptor family. It is structurally similar to, but distinct from, the insulin receptor, with which it shares a 70% homology. As expected, it crossreacts with insulin and, vice versa, insulin receptor crossreacts with IGF-1. Numerous studies suggest that IGF-1-R is very important for mitogenesis and is essential for phenotype transformation, at least in rodents (1). In particular, the IGF-1-R has been described in human breast cancer (2-4) and ovarian cancer (5) tissues and in cultured human breast cancer cell lines (6,7). Y1 - 2001 SP - 485 EP - 492 ER -