TY - JOUR A1 - Vinken, Mathieu A1 - Grimm, Daniela A1 - Baatout, Sarah A1 - Baselet, Bjorn A1 - Beheshti, Afshin A1 - Braun, Markus A1 - Carstens, Anna Catharina A1 - Casaletto, James A. A1 - Cools, Ben A1 - Costes, Sylvain V. A1 - De Meulemeester, Phoebe A1 - Doruk, Bartu A1 - Eyal, Sara A1 - Ferreira, Miguel J. S. A1 - Miranda, Silvana A1 - Hahn, Christiane A1 - Akyuz, Sinem Helvacioglu A1 - Herbert, Stefan A1 - Krepkiy, Dmitriy A1 - Lichterfeld, Yannik A1 - Liemersdorf, Christian A1 - Marcus, Krüger A1 - Marchal, Shannon A1 - Ritz, Jette A1 - Schmakeit, Theresa A1 - Stenuit, Hilde A1 - Tabury, Kevin A1 - Trittel, Torsten A1 - Wehland, Markus A1 - Zhang, Yu Shrike A1 - Putt, Karson S. A1 - Zhang, Zhong-Yin A1 - Tagle, Danilo A. T1 - Taking the 3Rs to a higher level: replacement and reduction of animal testing in life sciences in space research JF - Biotechnology advances N2 - Human settlements on the Moon, crewed missions to Mars and space tourism will become a reality in the next few decades. Human presence in space, especially for extended periods of time, will therefore steeply increase. However, despite more than 60 years of spaceflight, the mechanisms underlying the effects of the space environment on human physiology are still not fully understood. Animals, ranging in complexity from flies to monkeys, have played a pioneering role in understanding the (patho)physiological outcome of critical environmental factors in space, in particular altered gravity and cosmic radiation. The use of animals in biomedical research is increasingly being criticized because of ethical reasons and limited human relevance. Driven by the 3Rs concept, calling for replacement, reduction and refinement of animal experimentation, major efforts have been focused in the past decades on the development of alternative methods that fully bypass animal testing or so-called new approach methodologies. These new approach methodologies range from simple monolayer cultures of individual primary or stem cells all up to bioprinted 3D organoids and microfluidic chips that recapitulate the complex cellular architecture of organs. Other approaches applied in life sciences in space research contribute to the reduction of animal experimentation. These include methods to mimic space conditions on Earth, such as microgravity and radiation simulators, as well as tools to support the processing, analysis or application of testing results obtained in life sciences in space research, including systems biology, live-cell, high-content and real-time analysis, high-throughput analysis, artificial intelligence and digital twins. The present paper provides an in-depth overview of such methods to replace or reduce animal testing in life sciences in space research. Y1 - 2025 U6 - https://doi.org/10.1016/j.biotechadv.2025.108574 VL - 81 IS - 7/8 PB - Elsevier ER - TY - CHAP A1 - Andrzejewski, Dennis A1 - Jentsch, P. A1 - Markus, A. M. A1 - Tetzlaff, Laura A1 - Haeusler, N. A1 - Beck, Eberhard T1 - Entwicklung eines webbasierten Prototyps für die leitliniengerechte Entscheidungsunterstützung beim primären Mammakarzinom - Wie können Therapieempfehlungen und deren Begründungen optimal dokumentiert werden? BT - Meeting Abstract T2 - GMDS 2015. 60. Jahrestagung der Deutschen Gesellschaft für Medizinische Informatik, Biometrie und Epidemiologie e.V. (GMDS). Krefeld, 06.-09.09.2015 Y1 - 2015 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:0183-15gmds0704 PB - German Medical Science GMS Publishing House CY - Düsseldorf ER - TY - CHAP A1 - Andrzejewski, Dennis A1 - Jentsch, P. A1 - Markus, A. M. A1 - Tetzlaff, Laura A1 - Haeusler, N. A1 - Beck, Eberhard T1 - Therapeutische Entscheidungen beim Mammakarzinom − Wie können leitliniengerechte und nicht-leitliniengerechte Entscheidungen am besten dokumentiert werden? T2 - 35. Jahrestagung der Deutschen Gesellschaft für Senologie, Leipzig 2015 Y1 - 2015 ER - TY - CHAP A1 - Andrzejewski, Dennis A1 - Bollmann, M. A1 - Schulz, C. A1 - Markus, A. A1 - Jentsch, P. A1 - Häusler, N. A1 - Beck, Eberhard T1 - Konzeption eines am Behandlungsprozess orientierten Informationssystems für Brustkrebspatientinnen, deren Angehörige und Freunde T2 - 212. Tagung der Niederrheinisch-Westfälischen Gesellschaft für Gynäkologie und Geburtshilfe (NWGGG) Köln, 12.–13. Juni 2015 Y1 - 2015 ER - TY - CHAP A1 - Andrzejewski, Dennis A1 - Jentsch, P. A1 - Markus, A. M. A1 - Häusler, N. A1 - Tetzlaff, Laura A1 - Beck, Eberhard T1 - Entwicklung eines webbasierten Prototyps für die leitliniengerechte Entscheidungsunterstützung beim primären Mammakarzinom - Wie können Therapieempfehlungen und deren Begründungen optimal dokumentiert werden? T2 - Fortschritt durch Forschung : multidisziplinäre Wissenschaft in der GMDS ; 06. - 09. September 2015 an der Hochschule Niederrhein in Krefeld / GMDS. [Veranst. Deutsche Gesellschaft für Medizinische Informatik, Biometrie und Epidemiologie e.V.] Y1 - 2015 ER -