<?xml version="1.0" encoding="utf-8"?>
<export-example>
  <doc>
    <id>3329</id>
    <completedYear>2020</completedYear>
    <publishedYear/>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>1</pageFirst>
    <pageLast>10</pageLast>
    <pageNumber/>
    <edition/>
    <issue/>
    <volume/>
    <type>article</type>
    <publisherName>MDPI</publisherName>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>2025-05-08</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Symmetry Breaking and Emergence of Directional Flows in Minimal Actomyosin Cortices</title>
    <abstract language="eng">Cortical actomyosin flows, among other mechanisms, scale up spontaneous symmetry breaking and thus play pivotal roles in cell differentiation, division, and motility. According to many model systems, myosin motor-induced local contractions of initially isotropic actomyosin cortices are nucleation points for generating cortical flows. However, the positive feedback mechanisms by which spontaneous contractions can be amplified towards large-scale directed flows remain mostly speculative. To investigate such a process on spherical surfaces, we reconstituted and confined initially isotropic minimal actomyosin cortices to the interfaces of emulsion droplets. The presence of ATP leads to myosin-induced local contractions that self-organize and amplify into directed large-scale actomyosin flows. By combining our experiments with theory, we found that the feedback mechanism leading to a coordinated directional motion of actomyosin clusters can be described as asymmetric cluster vibrations, caused by intrinsic non-isotropic ATP consumption with spatial confinement. We identified fingerprints of vibrational states as the basis of directed motions by tracking individual actomyosin clusters. These vibrations may represent a generic key driver of directed actomyosin flows under spatial confinement in vitro and in living systems.</abstract>
    <parentTitle language="eng">Cells</parentTitle>
    <identifier type="url">https://www.mdpi.com/2073-4409/9/6/1432</identifier>
    <identifier type="doi">10.3390/cells9061432</identifier>
    <enrichment key="PeerReviewNachweis">ja</enrichment>
    <enrichment key="opus.source">publish</enrichment>
    <licence>Creative Commons - CC BY - Namensnennung 4.0</licence>
    <author>Sven Vogel</author>
    <author>Christian Wölfer</author>
    <author>Diego Ramirez-Diaz</author>
    <author>Robert Flassig</author>
    <author>Kai Sundmacher</author>
    <author>Petra Schwille</author>
    <collection role="open_access" number="">open_access</collection>
    <collection role="institutes" number="">Fachbereich Technik</collection>
    <collection role="Hochschulbibliografie" number="1">Hochschulbibliografie</collection>
    <thesisPublisher>Technische Hochschule Brandenburg</thesisPublisher>
  </doc>
  <doc>
    <id>2530</id>
    <completedYear>2019</completedYear>
    <publishedYear/>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>1800320</pageFirst>
    <pageLast/>
    <pageNumber/>
    <edition/>
    <issue>6</issue>
    <volume>3</volume>
    <type>article</type>
    <publisherName>Wiley</publisherName>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>2020-02-04</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Towards Design of Self‐Organizing Biomimetic Systems</title>
    <parentTitle language="eng">Advanced Biosystems</parentTitle>
    <identifier type="doi">/10.1002/adbi.201800320</identifier>
    <identifier type="issn">2366-7478</identifier>
    <enrichment key="PeerReviewNachweis">ja</enrichment>
    <licence>Keine Nutzungslizenz vergeben - es gilt das deutsche Urheberrecht</licence>
    <author>Robert  J. Flassig</author>
    <author>Michael Mangold</author>
    <author>Christian Wölfer</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>bottom-up</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>synthetic biology</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>functional biomodules</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>model-based bioengineering</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>protocell</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>whole cell model</value>
    </subject>
    <collection role="ddc" number="570">Biowissenschaften; Biologie</collection>
    <collection role="institutes" number="">Fachbereich Technik</collection>
    <collection role="Hochschulbibliografie" number="1">Hochschulbibliografie</collection>
    <thesisPublisher>Technische Hochschule Brandenburg</thesisPublisher>
  </doc>
</export-example>
