@unpublished{VogelWoelferRamirezDiazetal.2020, author = {Vogel, Sven K. and W{\"o}lfer, Christian and Ramirez-Diaz, Diego A. and Flassig, Robert J. and Sundmacher, Kai and Schwille, Petra}, title = {Symmetry breaking and emergence of directional flows in minimal actomyosin cortices}, doi = {10.20944/preprints202004.0008.v1}, pages = {10}, year = {2020}, language = {en} } @unpublished{FlassigVogelWoelferetal.2018, author = {Flassig, Robert J. and Vogel, Sven K. and W{\"o}lfer, Christian and Ramirez, Diego A. and Sundmacher, Kai and Schwille, Petra}, title = {Cortical Actomyosin Flows are Generated by Actomyosin Cluster Vibrationsin Vitro}, publisher = {bioRxiv / Cold Spring Harbor Laboratory}, doi = {10.1101/394700}, year = {2018}, language = {en} } @article{FachetWitteFlassigetal.2020, author = {Fachet, Melanie and Witte, Carina and Flassig, Robert J. and Rihko-Struckmann, Liisa K. and McKie-Krisberg, Zaid and Polle, J{\"u}rgen E. W. and Sundmacher, Kai}, title = {Reconstruction and analysis of a carbon-core metabolic network for Dunaliella salina}, series = {BMC Bioinformatics}, volume = {21}, journal = {BMC Bioinformatics}, number = {1}, publisher = {BioMed Central}, doi = {10.1186/s12859-019-3325-0}, year = {2020}, language = {en} } @article{VogelWoelferRamirezDiazetal.2020, author = {Vogel, Sven and W{\"o}lfer, Christian and Ramirez-Diaz, Diego and Flassig, Robert and Sundmacher, Kai and Schwille, Petra}, title = {Symmetry Breaking and Emergence of Directional Flows in Minimal Actomyosin Cortices}, series = {Cells}, journal = {Cells}, publisher = {MDPI}, doi = {10.3390/cells9061432}, pages = {1 -- 10}, year = {2020}, abstract = {Cortical actomyosin flows, among other mechanisms, scale up spontaneous symmetry breaking and thus play pivotal roles in cell differentiation, division, and motility. According to many model systems, myosin motor-induced local contractions of initially isotropic actomyosin cortices are nucleation points for generating cortical flows. However, the positive feedback mechanisms by which spontaneous contractions can be amplified towards large-scale directed flows remain mostly speculative. To investigate such a process on spherical surfaces, we reconstituted and confined initially isotropic minimal actomyosin cortices to the interfaces of emulsion droplets. The presence of ATP leads to myosin-induced local contractions that self-organize and amplify into directed large-scale actomyosin flows. By combining our experiments with theory, we found that the feedback mechanism leading to a coordinated directional motion of actomyosin clusters can be described as asymmetric cluster vibrations, caused by intrinsic non-isotropic ATP consumption with spatial confinement. We identified fingerprints of vibrational states as the basis of directed motions by tracking individual actomyosin clusters. These vibrations may represent a generic key driver of directed actomyosin flows under spatial confinement in vitro and in living systems.}, language = {en} }