@article{BeckHofmannBernhardtetal.1994, author = {Beck, Eberhard and Hofmann, M. and Bernhardt, G. and J{\"a}ger, W. and Wildt, L. and Lang, N.}, title = {In vitro activity of immunoconjugates between cisplatin and an anti CA125 monoclonal antibody on ovarian cancer cell lines}, series = {In: Cell Biophysics 24/25(1994)1-3, 163-173}, journal = {In: Cell Biophysics 24/25(1994)1-3, 163-173}, issn = {0163-4992}, pages = {163 -- 173}, year = {1994}, abstract = {Cis-diammine dichloro platinum (II) (CDDP), is a highly potent antineoplastic agent that is used in the treatment of ovarian cancer. However, the clinical use of CDDP is restricted by its severe side effects. In order to reduce these side effects and to enhance its therapeutic efficacy, we developed specific immunoconjugates consisting of the murine monoclonal antibody OC125 and CDDP, using diethylene triamine pentaacetic acid (DTPA) as a linker. The coupling efficiencies of the different preparations synthesized, varied between 1.10±0.42 and 2.65±1.60 mol of CDDP per mol of antibody protein. Despite the chemical modification of the antibody molecule, specific binding activity of the OC125-CDDP conjugates toward the CA125 antigen was maintained as was demonstrated by means of immunohisto-/cytochemical staining of frozen sections of ovarian cancer tissue, amniotic epithelium, and the CA125 positive ovarian cancer cell line NIH:OVCAR 3. The antiproliferative activity of the immunoconjugates was tested against the human ovarian cancer cell lines NIH:OVCAR3 and SKOV 3, applying a kinetic crystal violet microassay. Despite the promising results obtained with the specific immuno-staining of the target cells, no significant antiproliferative activity of our immunoconjugates against the cell lines tested was observed. One possible explanation for the lack of antitumor activity could be the fact that CA125 is released in large amounts by the NIH:OVCAR 3 cells. This may have prevented an efficient immunotargeting of the cancer cells by the formation of soluble immune complexes.}, language = {en} } @inproceedings{BeckHofmannKiesewetteretal.1992, author = {Beck, Eberhard and Hofmann, M. and Kiesewetter, F. and J{\"a}ger, W. and Wildt, L. and Lang, N.}, title = {Analysis of CA 125 release by ovarian cancer cells in vitro}, series = {In: Tumor associated antigens, oncogenes, receptors, cytokines in tumor diagnosis and therapy at the beginning of the nineties / 6th Symposium on Tumor Markers, Hamburg, [December 9. - 11. 1991] ; Satellite Symposium Cancer of the Breast - State and Trends in Diagnosis and Therapy. Ed. by R. - M{\"u}nchen [u.a.] : Zuckschwerdt, 1992. - ISBN 3-88603-444-5}, booktitle = {In: Tumor associated antigens, oncogenes, receptors, cytokines in tumor diagnosis and therapy at the beginning of the nineties / 6th Symposium on Tumor Markers, Hamburg, [December 9. - 11. 1991] ; Satellite Symposium Cancer of the Breast - State and Trends in Diagnosis and Therapy. Ed. by R. - M{\"u}nchen [u.a.] : Zuckschwerdt, 1992. - ISBN 3-88603-444-5}, pages = {117 -- 121}, year = {1992}, language = {en} }