Cardiovascular and Renal Effects of High Salt Diet in GDNF+/- Mice with Low Nephron Number

Language
en
Document Type
Article
Issue Date
2014-11-03
Issue Year
2013
Authors
Schlote, Julia
Schröder, Agnes
Dahlmann, Anke
Karpe, Britta
Cordasic, Nada
Daniel, Christoph
Hilgers, Karl Friedrich
Titze, Jens
Amann, Kerstin
Benz, Kerstin
Editor
Abstract

Aims: To test the suggested association of low nephron number and later development of renal and cardiovascular disease we investigated the effects of high sodium diet in heterozygous GDNF+/- mice. Methods: Aged wild type and GDNF+/- mice were grouped together according to high sodium (HS, 4%) or low sodium (LS, 0.03%) diet for 4 weeks. The heart, the aorta and the kidneys were processed for morphometric and stereological evaluations and TaqMan PCR. Results: On HS GDNF+/- mice showed significantly higher drinking volume and urine production than wt and mean arterial blood pressure tended to be higher. Heart weight was higher in GDNF+/- than in wt, but the difference was only significant for LS. HS significantly increased cardiac interstitial tissue in GDNF+/-, but not in wt. On LS GDNF+/- mice had significantly larger glomeruli than wt and HS led to an additional two fold increase of glomerular area compared to LS. On electron microscopy glomerular damage after HS was seen in GDNF+/-, but not in wt. Dietary salt intake modulated renal IL-10 gene expression in GDNF+/-. Conclusion: In the setting of 30% lower nephron number HS diet favoured maladaptive changes of the kidney as well as of the cardiovascular system.

Journal Title
Kidney & Blood Pressure Research
Volume
37
Issue
4/5
Citation
Kidney & Blood Pressure Research 2013; 37: 379-391. <http://www.karger.com/Article/Pdf/355716> © 2013 S. Karger AG, Basel
DOI
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