Genotoxic and cytotoxic potential of methacrylate-based orthodontic adhesives

Language
en
Document Type
Article
Issue Date
2023-05-25
First published
2021-05-01
Issue Year
2021
Authors
Taubmann, Andreas
Willershausen, Ines
Walter, Christian
Al-Maawi, Sarah
Kaina, Bernd
Gölz, Lina
Editor
Publisher
Springer Berlin Heidelberg
Abstract

Abstract

      Objectives
      The biocompatibility of methacrylate-based adhesives is a topic that is intensively discussed in dentistry. Since only limited evidence concerning the cyto- and genotoxicity of orthodontic adhesives is available, the aim of this study was to measure the genotoxic potential of seven orthodontic methacrylate-based adhesives.
    
    
      Materials and methods
      The XTT assay was utilized to determine the cytotoxicity of Assure Plus, Assure Bonding Resin, ExciTE F, OptiBond Solo Plus, Scotchbond Universal Adhesive, Transbond MIP, and Transbond XT after an incubation period of 24 h on human gingival fibroblasts. We also performed the γH2AX assay to explore the genotoxic potential of the adhesives within cytotoxic dose ranges after an incubation period of 6 h.
    
    
      Results
      The XTT assay showed a concentration-dependent reduction in cell viability. The decrease in cellular viability was in the same dose range most significant for Assure Plus, rendering it the adhesive material with the highest cytotoxicity. Employing the γH2AX assay, a concentration-dependent increase in H2AX phosphorylation was detected, indicating induction of DNA damage.
    
    
      Conclusions
      For most products, a linear correlation between the material concentration and γH2AX foci was observed. The most severe effect on γH2AX focus induction was found for Transbond MIP, which was the only adhesive in the test group containing the co-initiator diphenyliodonium hexafluorophosphate (DPIHP).
    
    
      Clinical relevance
      The data indicate that orthodontic adhesives, notably Transbond MIP, bear a genotoxic potential. Since the study was performed with in vitro cultivated cells, a direct translation of the findings to in vivo exposure conditions should be considered with great diligence.
Journal Title
Clinical Oral Investigations
Volume
25
Issue
5
Citation

Clinical Oral Investigations 25.5.(2021): 2569-2581. https://link.springer.com/article/10.1007/s00784-020-03569-x

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