In Vivo Validation of Spray-Dried Mesoporous Bioactive Glass Microspheres Acting as Prolonged Local Release Systems for BMP-2 to Support Bone Regeneration

Please always quote using this URN: urn:nbn:de:bvb:29-opus4-145247
  • Bone morphogenetic protein-2 (BMP-2) is a known key mediator of physiological bone regeneration and is clinically approved for selected musculoskeletal interventions. Yet, broad usage of this growth factor is impeded due to side effects that are majorly evoked by high dosages and burst release kinetics. In this study, mesoporous bioactive glass microspheres (MBGs), produced by an aerosol-assisted spray-drying scalable process, were loaded with BMP-2 resulting in prolonged, low-dose BMP-2 release without affecting the material characteristics. In vitro, MBGs were found to be cytocompatible and to induce a pro-osteogenic response in primary human mesenchymal stromal cells (MSCs). In aBone morphogenetic protein-2 (BMP-2) is a known key mediator of physiological bone regeneration and is clinically approved for selected musculoskeletal interventions. Yet, broad usage of this growth factor is impeded due to side effects that are majorly evoked by high dosages and burst release kinetics. In this study, mesoporous bioactive glass microspheres (MBGs), produced by an aerosol-assisted spray-drying scalable process, were loaded with BMP-2 resulting in prolonged, low-dose BMP-2 release without affecting the material characteristics. In vitro, MBGs were found to be cytocompatible and to induce a pro-osteogenic response in primary human mesenchymal stromal cells (MSCs). In a pre-clinical rodent model, BMP-2 loaded MBGs significantly enhanced bone formation and influenced the microarchitecture of newly formed bone. The MBG carriers alone performed equal to the untreated (empty) control in most parameters tested, while additionally exerting mild pro-angiogenic effects. Using MBGs as a biocompatible, pro-regenerative carrier for local and sustained low dose BMP-2 release could limit side effects, thus enabling a safer usage of BMP-2 as a potent pro-osteogenic growth factor.show moreshow less

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Metadaten
Author:Julia C. Berkmann, Aaron X. Herrera Martin, Carlotta Pontremoli, Kai Zheng, Christian H. Bucher, Agnes Ellinghaus, Aldo R. Boccaccini, Sonia Fiorilli, Chiara Vitale Brovarone, Georg N. Duda, Katharina Schmidt-Bleek
Persistent identifiers - URN:urn:nbn:de:bvb:29-opus4-145247
Persistent identifiers - DOI:https://doi.org/10.3390/pharmaceutics12090823
ISSN:1999-4923
Title of the journal / compilation (English):Pharmaceutics
Publisher:MDPI
Document Type:Article
Language:English
Year of publication:2020
Date of first Publication:2020/08/28
Publishing Institution:Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU)
Release Date:2020/09/04
Tag:BMP-2; autologous blood clot; fracture healing; local drug release; mesoporous bioactive glass
Volume/year:12
Issue:9
Original publication:Pharmaceutics 12.9 (2020). <https://www.mdpi.com/1999-4923/12/9/823>
Institutes:Technische Fakultät
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
open_access (DINI-Set):open_access
Collections:Universität Erlangen-Nürnberg / Eingespielte Open Access Artikel / Eingespielte Open Access Artikel 2020
Licence (German):Creative Commons - CC BY - Namensnennung 4.0 International