Lateral Spacing of TiO2 Nanotubes Modulates Osteoblast Behavior

Please always quote using this URN: urn:nbn:de:bvb:29-opus4-128563
  • Titanium dioxide (TiO2) nanotube coated substrates have revolutionized the concept of implant in a number of ways, being endowed with superior osseointegration properties and local drug delivery capacity. While accumulating reports describe the influence of nanotube diameter on cell behavior, little is known about the effects of nanotube lateral spacing on cells involved in bone regeneration. In this context, in the present study the MC3T3-E1 murine pre-osteoblast cells behavior has been investigated by using TiO2 nanotubes of ~78 nm diameter and lateral spacing of 18 nm and 80 nm, respectively. Both nanostructured surfaces supported cell viability and proliferation in approximately equalTitanium dioxide (TiO2) nanotube coated substrates have revolutionized the concept of implant in a number of ways, being endowed with superior osseointegration properties and local drug delivery capacity. While accumulating reports describe the influence of nanotube diameter on cell behavior, little is known about the effects of nanotube lateral spacing on cells involved in bone regeneration. In this context, in the present study the MC3T3-E1 murine pre-osteoblast cells behavior has been investigated by using TiO2 nanotubes of ~78 nm diameter and lateral spacing of 18 nm and 80 nm, respectively. Both nanostructured surfaces supported cell viability and proliferation in approximately equal extent. However, obvious differences in the cell spreading areas, morphologies, the organization of the actin cytoskeleton and the pattern of the focal adhesions were noticed. Furthermore, investigation of the pre-osteoblast differentiation potential indicated a higher capacity of larger spacing nanostructure to enhance the expression of the alkaline phosphatase, osteopontin and osteocalcin osteoblast specific markers inducing osteogenic differentiation. These findings provide the proof that lateral spacing of the TiO2 nanotube coated titanium (Ti) surfaces has to be considered in designing bone implants with improved biological performance.show moreshow less

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Metadaten
Author:Madalina Georgiana Necula, Anca Mazare, Raluca Nicoleta Ion, Selda Ozkan, Jung Park, Patrik Schmuki, Anisoara Cimpean
URN:urn:nbn:de:bvb:29-opus4-128563
DOI:https://doi.org/10.3390/ma12182956
ISSN:1996-1944
Title of the journal / compilation (English):Materials
Publisher:MDPI
Document Type:Article
Language:English
Year of Completion:2019
Date of first Publication:2019/09/12
Publishing Institution:Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU)
Release Date:2020/01/15
Tag:cell adhesion and morphology; cell proliferation; osteoblast; osteogenic differentiation; spaced TiO2 nanotubes
Volume:12
Issue:18
Original publication:Materials 12.18 (2019). <https://www.mdpi.com/1996-1944/12/18/2956>
Institutes:Technische Fakultät
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 60 Technik / 600 Technik, Technologie
open_access (DINI-Set):open_access
Collections:Universität Erlangen-Nürnberg / Eingespielte Open Access Artikel / Eingespielte Open Access Artikel 2020
Licence (German):Creative Commons - CC BY - Namensnennung 4.0 International