Macromolecular interactions in alginate–gelatin hydrogels regulate the behavior of human fibroblasts

Language
en
Document Type
Article
Issue Date
2019-06-14
Issue Year
2017
Authors
Sarker, Bapi
Singh, Raminder
Zehnder, Tobias
Forgber, Tim
Alexiou, Christoph
Cicha, Iwona
Detsch, Rainer
Boccaccini, Aldo R.
Editor
Abstract

Due to the presence of tripeptide arginine–glycine–aspartic acid, gelatin is considered a very promising additive material to improve the cytocompatibility of alginate-based hydrogels. Two different strategies, physical blending and chemical crosslinking with gelatin, are used in this study to modify alginate hydrogel. As the intermolecular interactions between the polysaccharide and protein in the resulting physically blended and chemically crosslinked hydrogels are different, significant differences in the properties of these hydrogel types, regarding especially their surface topography, degradation kinetics, mechanical properties, and protein release behavior, are observed. Cellular behavior on both types of alginate–gelatin hydrogels is investigated using primary human dermal fibroblasts to elucidate the effects of the different structural, mechanical, and degradation properties of the produced hydrogels on fibroblast attachment and growth. The hydrogel that is chemically crosslinked with gelatin exhibits the highest degree of cytocompatibility regarding adhesion, proliferation, metabolic activity, and morphology of growing fibroblasts.

Journal Title
Journal of Bioactive and Compatible Polymers
Volume
32
Issue
3
Citation
Bioactive and Compatible Polymers 32.3 (2017): S. 309 - 324. <https://journals.sagepub.com/doi/full/10.1177/0883911516668667>
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