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Groundwater is an important resource in Sub-Saharan Africa to supply water for domestic, industrial, mining and irrigation for agricultural purposes. In Ghana, groundwater supply program is mostly pragmatic to give people drinking water, however to ensure sustainability a long term groundwater management system is essential. One such approach is the use source protection (well protection) with the declaration and installation of protection zones. To achieve the goal of this research three hypothetical questions were asked which relates to the Availability and Vulnerability of groundwater, and the use of an analytical model to estimate source protection zones (SPZ). The regions of Adansi, Lawra, Sunyani and Sekyere West were chosen as study areas to test the hypothesis of this research with detailed investigation concentrating on Adansi area. The overall aim from the study areas was to estimate SPZ for Granite, Phyllite, Schist, Sandstone and weathered materials and give recommendations as to which activity should be permitted or otherwise within each of these zones. The available groundwater for a particular well or location was determined based on its hydraulic conductivity and specific capacity of a well. Regionalization of the available groundwater was achieved by comparing the results of the hydraulic conductivity and specific capacity with the regional structural map. To determine the vulnerability of the available groundwater, the radius of influence (ROI) for the different boreholes was determined using Sichard and Dupuits formula whilst for Source protection zones, Darcy and the volumetric equation was used. Results of hydraulic conductivity estimated from pumping test data for the five major rock types showed a trimodal distribution (E-5, E-6 and E-7 m/s). There was a general trend of decreasing hydraulic conductivity to decreasing transmissivity. Results of plots of specific capacity verses hydraulic conductivity shows that only rock types with hydraulic conductivity greater than E-5 m/s yields sustainable groundwater supply. Three aquifer types were encountered in the study areas which were pore aquifers in shallow or alluvial, regolith and fracture aquifers in bedrocks. High hydraulic conductivity from the different rock types corresponds to higher ROI. The results of hydraulic conductivity, specific capacity, ROI and SPZ are used as input parameters for the estimation of the suitability of a groundwater source. A ranking matrix was developed for easy interpretation of hydrogeological data by combining the results of the input parameters into a single score. Results from the ranking matrix were used to determine specific regulations for the various aquifers in the study areas. Within the fractured crystalline rocks with k ≥E-5m/s siting of wells should target fracture zones whilst pumping during abstraction phase can exceed 15m3/s/m. The intermediate source protection zone should be at least 150m. Conclusions from the study could be applied in other areas of Ghana and be implemented during the initial development, abstraction and groundwater policy phases of other groundwater projects.