TY - JOUR A1 - Otto, Pascal A1 - Alipoursarbani, Mozhdeh A1 - Torrent, Daniel A1 - Latorre-Pérez, Adriel A1 - Paust, Thomas A1 - Albert, Alfred A1 - Abendroth, Christian T1 - Microbiome characterization after aerobic digestate reactivation of anaerobically digested sewage sludge T1 - Mikrobiomcharakterisierung nach aerober Gärrestreaktivierung von anaerob vergärtem Klärschlamm N2 - A demonstrator plant of a recently patented process for improved sludge degradation has been implemented on a municipal scale. In a 1500 m3 sewage sludge digester, an intermediary stage with aerobic sewage sludge reactivation was implemented. This oxic activation increased the biogas yield by up to 55% with a 25% reduction of the remaining fermentation residue volume. Furthermore, this process allowed an NH4-N removal of over 90%. Additionally, 16S rRNA gene amplicon high-throughput sequencing of the reactivated digestate showed a reduced number of methane-forming archaea compared to the main digester. Multiple ammonium-oxidizing bacteria were detected. This includes multiple genera belonging to the family Chitinophagaceae (the highest values reached 18.8% of the DNA sequences) as well as a small amount of the genus Candidatus nitrosoglobus (<0.3%). In summary, the process described here provides an economically viable method to eliminate nitrogen from sewage sludge while achieving higher biogas yields and fewer potential pathogens in the residuals. N2 - Eine Demonstrationsanlage eines kürzlich patentierten Verfahrens zur verbesserten Schlammzersetzung wurde im kommunalen Maßstab umgesetzt. In einem 1500 m3 Klärschlammfaulbehälter wurde eine Zwischenstufe mit aerober Klärschlammreaktivierung implementiert. Diese oxische Aktivierung steigerte die Biogasausbeute um bis zu 55 % bei einer Reduzierung des verbleibenden Gärrestvolumens um 25 %. Darüber hinaus ermöglichte dieser Prozess eine NH4-N-Entfernung von über 90 %. Weiterhin zeigte die 16S-rRNA-Gen-Amplikon-Hochdurchsatzsequenzierung des reaktivierten Gärrestes eine geringere Anzahl methanbildender Archaeen im Vergleich zum Hauptfermenter. Es wurden mehrere Ammonium-oxidierende Bakterien nachgewiesen. Dazu gehören mehrere Gattungen der Familie Chitinophagaceae (die höchsten Werte erreichten 18,8 % der DNA-Sequenzen) sowie eine kleine Menge der Gattung Candidatus nitrosoglobus (<0,3 %). Zusammenfassend stellt das hier beschriebene Verfahren eine wirtschaftlich sinnvolle Methode dar, um Stickstoff aus Klärschlamm zu eliminieren und gleichzeitig höhere Biogasausbeuten und weniger potenzielle Krankheitserreger in den Reststoffen zu erzielen. KW - Anaerobic digestion KW - Anaerobic microbiomes KW - Aerobic sludge activation KW - 16S rRNA sequencing KW - Water treatment KW - Belebtschlammverfahren KW - Schlammfaulung KW - Mikroorganismus KW - Gärung KW - Biogas KW - Stickstoff-Elimination KW - Anaerobe Vergärung KW - Anaerobe Mikrobiome KW - Aerobe Schlammaktivierung KW - 16S-rRNA-Sequenzierung KW - Wasserversorgung Y1 - 2023 U6 - https://doi.org/10.3390/fermentation9050471 SN - 2311-5637 VL - 9 IS - 5 PB - MDPI CY - Basel ER - TY - JOUR A1 - Otto, Pascal A1 - Puchol‑Royo, Roser A1 - Ortega‑Legarreta, Asier A1 - Tanner, Kristie A1 - Tideman, Jeroen A1 - Vries, Sjoerd‑Jan de A1 - Pascual, Javier A1 - Porcar, Manuel A1 - Latorre‑Pérez, Adriel A1 - Abendroth, Christian T1 - Multivariate comparison of taxonomic, chemical and operational data from 80 different full‑scale anaerobic digester‑related systems JF - Biotechnology for Biofuels and Bioproducts N2 - The holistic characterization of different microbiomes in anaerobic digestion (AD) systems can contribute to a better understanding of these systems and provide starting points for bioengineering. The present study investigates the microbiome of 80 European full-scale AD systems. Operational, chemical and taxonomic data were thoroughly collected, analysed and correlated to identify the main drivers of AD processes. The present study describes chemical and operational parameters for a broad spectrum of different AD systems. With this data, Spearman correlation and differential abundance analyses were applied to narrow down the role of the individual microorganisms detected. The authors succeeded in further limiting the number of microorganisms in the core microbiome for a broad range of AD systems. Based on 16S rRNA gene amplicon sequencing, MBA03, Proteiniphilum, a member of the family Dethiobacteraceae, the genus Caldicoprobacter and the methanogen Methanosarcina were the most prevalent and abundant organisms identified in all digesters analysed. High ratios for Methanoculleus are often described for agricultural co-digesters. Therefore, it is remarkable that Methanosarcina was surprisingly high in several digesters reaching ratios up to 47.2%. The various statistical analyses revealed that the microorganisms grouped according to different patterns. A purely taxonomic correlation enabled a distinction between an acetoclastic cluster and a hydrogenotrophic one. However, in the multivariate analysis with chemical parameters, the mainclusters corresponded to hydrolytic and acidogenic microorganisms, with SAOB bacteria being particularly important in the second group. Including operational parameters resulted in digester-type specific grouping of microbes. Those with separate acidification stood out among the many reactor types due to their unexpected behaviour. Despite maximizing the organic loading rate in the hydrolytic pretreatments, these stages turned into extremely robust methane production units. From 80 different AD systems, one of the most holistic data sets is provided. A very distinct formation of microbial clusters was discovered, depending on whether taxonomic, chemical or operational parameters were combined. The microorganisms in the individual clusters were strongly dependent on the respective reference parameters. KW - Anaerobic digestion KW - 16S rRNA sequencing KW - Core microbiome KW - Multivariate analysis KW - Parameter dependency KW - Gärung KW - Mikrobiom KW - Multivariate Analyse KW - Laborparameter Y1 - 2024 U6 - https://doi.org/10.1186/s13068-024-02525-1 SN - 2731-3654 VL - 17 PB - BMC CY - London ER -