@phdthesis{MatbaechiEttehad2021, author = {Matbaechi Ettehad, Honeyeh}, title = {Dielectrophoretic manipulation of yeast cells using CMOS integrated microfluidic}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:co1-opus4-55315}, school = {BTU Cottbus - Senftenberg}, year = {2021}, abstract = {The rapid detection of infectious diseases is still an unsolved problem since their identification must be carried out either by cultivation or DNA analysis in a laboratory. The development of point-of-care (PoC) is a current development trend that requires further technological impulses to produce reliable and cost-effective systems. By miniaturizing and integrating microfluidic and electronic components, the advantages of electronic methods can be transferred to the field of PoC testing. The combination of complementary metal-oxide-semiconductor (CMOS) technology with microfluidic platforms allowed the development of fully functional sample-to-result LoC setups, which served the portability of the device even out of the laboratory or hospitals. CMOS-based LoC device can control and manage the data from sensors, microfluidics, and actuators. Dielectrophoresis (DEP) is a non-destructive and non-invasive method promising to be used in PoC medical applications. Utilizing MEMS technology and fabrication of microelectrodes allow DEP to be applied in biomedical applications such as cell manipulation and separation with high speed, sensitivity and without any labeling. Cell detection and separation occupy an important place in diagnostics of viral and infectious diseases such as Influenza and COVID-19. Therefore, rapid, sensitive, and automated LoC devices are needed to detect such diseases. Starting from this point of view, manipulating the cells as a way to detect them using DEP was decided as the main objective of the thesis. This work aimed at developing a miniaturized CMOS integrated silicon microfluidic device, in line with a standard CMOS procedure, for characterization and manipulation of live and dead yeast cells using the DEP technique. Understanding the relationship between the microelectrode's geometry and the magnitude of DEP force, the microfluidic devices can be designed to produce the most effective DEP implication on biological samples. In this work, interdigitated electrode arrays (IDEs) were used to manipulate the cells. This microelectrode was primarily used to detect microorganisms in a solution, based on the measurement of the variation of the dielectric constant by the concentration of the microorganisms. Therefore, finite element simulations were performed to optimize this microelectrode and adapt it to our application. Thus, the IDEs were optimized as a function of finger width and spacing between adjacent fingers. One of the most serious matters related to DEP-based microfluidic devices is that the DEP spectra of the targeted cell should precisely be known. Therefore, the DEP spectrum analysis of various cell suspensions with different medium conductivities was studied comprehensively by finite element simulation and experimentally. This study presented an optimized trapping platform for both detection and separation applications in terms of electrode dimension and electrical parameters.}, subject = {Dielectrophoresis (DEP); Yeast cell; Cell manipulation; Interdigitated electrodes (IDEs); CMOS-integrated microfluidic Lab-on-a-chip; Dielektrophorese (DEP); Hefezelle; Zellmanipulation; Interdigitalelektroden (IDEs); CMOS-integriertes mikrofluidisches Lab-on-a-chip; CMOS; Lab on a Chip; Dielektrophorese; Saccharomyces cerevisiae; Mikrofluidik; Biomedizinische Technik}, language = {en} }