@book{RocktaeschelPalenKyasetal.2024, author = {Rockt{\"a}schel, Benjamin and Palen, Astrid and Kyas, Phillipp and Carlotto, Nicolas and Werle Vogel, Felipe and Otto, Pascal and Tideman, Jeroen and Jan de Vries, Sjoerd and Suleiman, Afnan and Eriksen, Freya and Abendroth, Christian and Alajami, Mutaz and Fern{\´a}ndez Tu{\~n}{\´o}n, Marina and Puchol-Royo, Roser and Latorre-P{\´e}rez, Adriel and Shyan Baptist, Savannah}, title = {The anaerobic microverse : an in-depth roadmap on AD plant operations, microbiome innovation, and economic impact}, editor = {Abendroth, Christian and Otto, Pascal and Fern{\´a}ndez, Camino and Mendoza, Ariadna and Branco, Joana}, doi = {10.26127/BTUOpen-6923}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:co1-opus4-69238}, publisher = {BTU Cottbus - Senftenberg}, year = {2024}, abstract = {The MICRO4BIOGAS roadmap provides a concise overview of the European biogas sector, placing the spotlight on the microbiome as a key factor in improving biogas production. Central to this is bioaugmentation - the targeted introduction of high-performing microorganisms into biogas plants to optimize biological processes. Funded by the EU under the Horizon 2020 program, the project brings together 14 partners from six countries. One of its major achievements is a comprehensive metagenomic analysis of 80 samples, offering one of the most in-depth insights to date into the microbial communities of European biogas plants. The roadmap highlights the role of key microbial groups - including bacteria, archaea, fungi, and bacteriophages - and their contribution to the efficiency of anaerobic digestion. It also outlines the state of biogas development in selected EU countries and profiles relevant stakeholders in the sector. Beyond its technical focus, MICRO4BIOGAS aims to raise awareness of the crucial role microbiomes play in biogas production. A Europe-wide survey involving over 200 stakeholders and a collaboration with the Spanish municipality of Aras de los Olmos, which is committed to renewable energy, further enrich the project.}, subject = {Biogas; Anaerobic digestion; Microbiome; MICRO4BIOGAS; Anaerobe Mikrobiome; Biomasseverg{\"a}rung; Biogasanlage; Mikroorganismus; Bioremediation; Metagenomik}, language = {en} } @article{OttoAlipoursarbaniTorrentetal.2023, author = {Otto, Pascal and Alipoursarbani, Mozhdeh and Torrent, Daniel and Latorre-P{\´e}rez, Adriel and Paust, Thomas and Albert, Alfred and Abendroth, Christian}, title = {Microbiome characterization after aerobic digestate reactivation of anaerobically digested sewage sludge}, volume = {9}, number = {5}, publisher = {MDPI}, address = {Basel}, issn = {2311-5637}, doi = {10.3390/fermentation9050471}, year = {2023}, abstract = {A demonstrator plant of a recently patented process for improved sludge degradation has been implemented on a municipal scale. In a 1500 m3 sewage sludge digester, an intermediary stage with aerobic sewage sludge reactivation was implemented. This oxic activation increased the biogas yield by up to 55\% with a 25\% reduction of the remaining fermentation residue volume. Furthermore, this process allowed an NH4-N removal of over 90\%. Additionally, 16S rRNA gene amplicon high-throughput sequencing of the reactivated digestate showed a reduced number of methane-forming archaea compared to the main digester. Multiple ammonium-oxidizing bacteria were detected. This includes multiple genera belonging to the family Chitinophagaceae (the highest values reached 18.8\% of the DNA sequences) as well as a small amount of the genus Candidatus nitrosoglobus (<0.3\%). In summary, the process described here provides an economically viable method to eliminate nitrogen from sewage sludge while achieving higher biogas yields and fewer potential pathogens in the residuals.}, subject = {Anaerobic digestion; Anaerobic microbiomes; Aerobic sludge activation; 16S rRNA sequencing; Water treatment; Belebtschlammverfahren; Schlammfaulung; Mikroorganismus; G{\"a}rung; Biogas; Stickstoff-Elimination; Anaerobe Verg{\"a}rung; Anaerobe Mikrobiome; Aerobe Schlammaktivierung; 16S-rRNA-Sequenzierung; Wasserversorgung}, language = {en} }