This paper analyses the Serbian socio-economic spatial differentiation via a purely quantitative approach. In Serbia, huge development disparities between north and south, urban and rural as well as central and peripheral areas are obvious. Getting to the bottom of these spatial differences is one of the fundamental scientific and planning challenges of the region. This question of unevenly distributed living conditions is examined on the basis of factor analyses of secondary statistical data of the Serbian municipalities. The intention thereby was to identify the distribution of regional or spatial inequality by correlating an amount of 25 reliable variables. Combining the spheres of population structure, economy, employment, infrastructure, pre-school education and medical care an overall Index of Socio-economic Pressure is proposed to detect patterns of regional development on the Serbian territory.