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With the latest ICP-MS technology - ICP-ToF (time of flight)-MS - it is possible to analyze the multi-element fingerprint of individual cells. The interface between material and environmental analysis thus receives special attention, e.g. when considering corrosion processes. Microbiologically influenced corrosion (MIC) is highly unpredictable due to the diversity of microbial communities involved. The development of the MIC-specific ICP-ToF-MS analytical method presented here at the single cell level, in combination with the investigation of steel-MIC interactions, contributes significantly to progress in instrumental MIC analysis and will enable clarification of the processes taking place.
The supplied document shows the basis of a four minutes lightning talk.
The latest ICP-MS technology - ICP-ToF (time of flight)-MS – enables the analysis of the multi-element fingerprint of individual cells. The interface between material and environmental analysis thus receives special attention, e.g., when considering corrosion processes. Microbiologically influenced corrosion (MIC) is a highly unpredictable phenomenon due to the influence of the environment, microbial communities involved and the respective electron source. However, the interaction pathway between cells and the metal surface remains unclear. The development of the MIC-specific ICP-ToF-MS analytical method presented here at the single cell level, in combination with the investigation of steel-MIC interactions, contributes significantly to progress in instrumental MIC analysis and will enable clarification of the processes taking place. For this, a MIC-specific staining procedure was developed. It allows the analysis of archaea at a single cell level and provides information about the interaction of the cells with the staining agent which is extremely scarce compared to other well characterized organisms. Additionally, the single cell ICP-ToF-MS is used for the analysis of archaea involved in MIC of steel. Hence, the possible uptake of individual elements from different steel samples is investigated - the information obtained will be used in the future to elucidate underlying mechanisms and develop possible material protection concepts, thus combining modern methods of analytical sciences with materials.
Materials in contact with the environment release e.g., metal-ions, elemental species and/or (nano-)particles. Once these species and/or particles are released, they are ingested by organisms and cells and thus, might have a negative impact on the environment. Thus, identification as well as quantification of potentially harmful substances is of utmost importance and highly needed to assess ecotoxicological impact of (emerging) pollutants.
The oral presentation provides an overview on the power of elemental analytical techniques, in particular ICP-MS as well as HR-CS-GFMAS in environmental research. Current research topics from Division 1.1 @ BAM - Inorganic trace analysis will be highlighted:
i) Elemental Speciation & Isotope analysis - new tools:
Among elemental species separation and quantification, one of the main challenges in environmental elemental speciation analysis is the distinction between anthropogenic and natural elemental species. The on-line combination of elemental speciation and isotope analysis combines “the best from both worlds” - species specific isotopic information becomes available.
As an application example the analysis of current anti-fouling agents via CE/MCICP-MS will be highlighted.
ii) HR-CS-GFMAS for PFC analysis:
Per- and polyfluorinated compounds (PFC) are emerging contaminants in particular in soil and surface water samples. Due to the large number of compounds (>4700), target analytical methods are not sufficient and sum parameter methods for organically bound fluorine are highly needed.
High resolution-continuum source-graphite furnace molecular absorption spectroscopy (HR-CS-GFMAS) based methods for organically bound fluorine analysis will be presented. Application examples (soil and surface water) will be highlighted.
iii) Single cell-ICP-ToF-MS - ecotox. assessment:
Single cell and single organism analysis for e.g. ecotoxicological/medicinal assessment are hot topics in the research field of ICP-MS. In particular ICP-ToF-MS is a powerful, emerging techniques in terms of single cell/particle analysis.
Automated single cell/diatom-ICP-ToF-MS as a potential tool in ecotoxicological testing will be presented.