Sanitär- und Kommunaltechnik; Umwelttechnik
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Organisationseinheit der BAM
- 4 Material und Umwelt (353)
- 1 Analytische Chemie; Referenzmaterialien (151)
- 4.1 Biologische Materialschädigung und Referenzorganismen (121)
- 6 Materialchemie (94)
- 8 Zerstörungsfreie Prüfung (80)
- 7 Bauwerkssicherheit (76)
- 1.8 Umweltanalytik (69)
- 8.1 Sensorik, mess- und prüftechnische Verfahren (67)
- 4.3 Schadstofftransfer und Umwelttechnologien (65)
- 4.0 Abteilungsleitung und andere (61)
Homochirality is an obvious feature of life on Earth. On the other hand, extraterrestrial samples contain largely racemic compounds. The same is true for any common organic synthesis. Therefore, it has been a perplexing puzzle for decades how these racemates could have formed enantiomerically enriched fractions as a basis for the origin of homochiral life forms. Numerous hypotheses have been put forward as to how preferentially homochiral molecules could have formed and accumulated on Earth. In this article, it is shown that homochirality of the abiotic organic pool at the time of formation of the first self-replicating molecules is not necessary and not even probable. It is proposed to abandon the notion of a molecular ensemble and to focus on the level of individual molecules. Although the formation of the first self-replicating, most likely homochiral molecule is a seemingly improbable event, on a closer look, it is almost inevitable that homochiral molecules have formed simply on a statistical basis. In this case, the non-selective leap to homochirality would be one of the first steps in chemical evolution directly out of a racemic “ocean”. Moreover, most studies focus on the chirality of the primordial monomers with respect to an asymmetric carbon atom. However, any polymer with a minimal size that allows folding to a secondary structure, would spontaneously lead to asymmetric higher structures (conformations). Most of the functions of these polymers would be influenced by this inherently asymmetric folding. To summarize, simple and universal mechanisms may have led to homochiral self-replicating systems in the context of chemical evolution. A homochiral monomer pool is deemed unnecessary and probably never existed on primordial Earth.
Organotin compounds (OTCs) have been widely used in anti-fouling paints, pesticide formulations, and as stabilizers in polyvinyl chloride over the past century. In marine ecosystems, OTCs can cause severe damage to biodiversity, leading up to the extinction of vulnerable species. Due to the extensive use of tributyltin (TBT) as a biocide on ship hulls, it has been considered one of the most hazardous substances intentionally introduced into the aquatic environment. This resulted in a global ban on TBT-containing products in the 2000s. However, recent studies indicate the emerging presence of organotin pollutants.
OTCs are known to persist and accumulate in marine sediments, posing a long-term threat to the environment. These harmful substances can be set free and dispersed even after several decades. Therefore, analyzing sediment probes is imperative for a thorough monitoring of pollution. However, species-specific analysis of OTCs at required concentration levels in complex environmental matrices remains challenging. Chromatographic systems are commonly used for their analysis, but the required sample preparation is time-consuming and prone to contamination and analyte loss. The coupling of electrothermal vaporization and inductively coupled plasma-mass spectrometry (ETV/ICP-MS) demonstrates high potential as a rapid, convenient, and chemical-saving scanning tool for environmental samples.
This method enables the direct on-line fractionation of organic compounds from an inorganic fraction and provides element-specific detection at ultra-trace levels without complex sample preparation. Since OTCs are generally more toxic than ionic or elemental tin, analyzing them as a sum parameter is advantageous. Additionally, the determination of both organic and inorganic tin, rather than just organic tin, reveals valuable information about the fate of OTCs. The main challenge in obtaining accurate quantitative data using direct solid sampling techniques like as ETV/ICP-MS is applying a suitable calibration strategy. Our isotope dilution approach overcomes matrix effects in ETV/ICP-MS analysis and is compatible with commercial systems.
PFAS (Per- and polyfluoroalkyl substances) are a widespread and emerging environmental problem due to their longevity and ubiquitous spread. The common structural motif of this compound class is the fluorine – carbon bond on an alkyl backbone, which are known for their chemical inertness and combination of hydrophobicity and oleophobicity. The downside of those properties is the general lack of usable molecular interactions for adsorption to extract them from the environment. The notable exception to that inertness are fluorine – fluorine interactions, which are stronger, the longer the perfluorinated chain length is. Therefore, in this project we investigated the effect of fluorination, length and presence of a charge on the alkyl chain on an adsorber. Towards that goal, in total 16 different adsorber materials were synthesized (fluorinated/non-fluorinated; C4/ C6/ C8/ C10 ; positive charge/no charge) and tested in regard to their potential to adsorb PFOA and perfluoralkylic acids of differing chain lengths. The PFAS concentration was tested via LC-MS/MS to determine the adsorption rate on the various materials. Further work includes the transition from a target analytics to sum parameter analysis for future evaluation of adsorber materials in PFAS environmental samples like river samples. Therefore a quarterly sampling and extraction of spree surface water samples was planned.
Per- and polyfluoroalkyl substances (PFAS) have received global public attention because of their wide distribution in aquatic environments and potential adverse effects to humans and wildlife. Due to the lack of analytical standards and the enormous numbers of these compounds, current target-based methods (e.g., LC-MS/MS) are not suitable for the analysis of new/unknown PFAS and transformation products. Therefore, PFAS sum parameter analysis is becoming increasingly important. For PFAS determination of surface waters, solid phase extraction (SPE) is commonly implemented for clean-up and pre-concentration of samples.
Thus, within this work, single-layer SPE methods (based on Strata™-X, Strata™-X-AW, Strata™-NH2 and Oasis-HLB) were investigated for maximum PFAS coverage utilizing extractable organically bound fluorine (EOF) analysis. Thereby, the optimization procedure relied on the analysis of 3 surface water samples in Berlin, Germany, which were affected by the effluent discharge of wastewater treatment plants. To analyse the SPE elution profiles for EOF and inorganic fluorine, high resolution-continuum source-graphite furnace molecular absorption spectroscopy (HR-CS-GFMAS) and ion chromatography (IC) were used, respectively.
Highest EOF concentrations were achieved by using Strata-X/Strata-XAW as SPE sorbents and methanol as eluent. Furthermore, combinations of the most promising SPE sorbents were selected (X/XAW, XAW/X, HLB/X and WAX/GCB) to extract a wider range of PFASs. By comparing multi-layer SPE methods, lower EOF concentrations were obtained for all investigated combination phases compared to the analysed single phases. For the commercially available combination phase Strata-PFAS (WAX/GCB) for all three river water samples, lower EOF concentrations (1.5 times lower) were determined compared to Strata-X.
The results have shown that single-layer SPE systems are currently superior compared to combination phases for PFAS sum parameter analysis. The multi-layer SPE methods need further optimization regarding appropriate sorbent combinations, loading volumes and elution conditions. The developed single-layer SPE methods can help to elucidate pollutions hotspots and discharge routes.
Advances in ultrafast laser manufacturing: nanostructures, thin films, and scaling perspectives
(2024)
Advanced ultrafast laser technology is a rapidly growing field that currently enables many new industrial and scientific applications. During the last decades, this has been significantly driven by the availability of high-repetition-rate laser sources and novel beam delivery concepts. At the laser side, Moore’s law equally manifests for ultrafast laser technologies, since the average output power of such lasers doubles approximately every two years. This development is mainly driven by the increase of the pulse repetition rates of energetic laser pulses, currently enforcing the development of smart beam control and novel scanning strategies for preventing heat-accumulation and plasma-shielding effects during laser-based materials processing. This keynote presentation addresses the advantages, recent developments, and perspectives of laser processing with ultrashort laser pulses. A special focus is laid on the tailored structuring of thin films as well as the manufacturing and probing of sub-diffraction surface nanostructures – an ongoing race to extreme scales. Current limitations are identified and an outlook to future scaling perspectives will be provided.
Since 2016 ISO Technical Committee (TC) 265 is working on standardization the whole CCS-process chain. Around 30 countries are working together to provide international guidelines. As ISO 27913 was the first standard finished within these activities it is already in the stage of first revision.
The objective of ISO 27913 is “to provide specific requirements and recommendations on certain aspects of safe and reliable design, construction and operation of pipelines intended for the large-scale transportation of CO2 that are not already covered in existing pipeline standards such as ISO 13623, ASME B31.8, EN 1594, AS 2885 or other standards. Existing pipeline standards cover many of the issues related to the design and construction of CO2 pipelines; however, there are some CO2 specific issues that are not adequately covered in these standards. The purpose of this document is to cover these issues consistently. Hence, this document is not a standalone standard, but is written to be a supplement to other existing pipeline standards for natural gas or liquids for both onshore and offshore pipelines.”
This contribution shall provide information on the content, the current stage of the revision process, encourage to contribute to this standard and make listeners aware to consider the influence of ISO standard on documents currently in draft.
Sunlight is an almost unavoidable environmental cue and plays a fundamental role in the biology of pro- and eukaryotic organisms. To cope with sunlight-associated stresses e.g., high temperatures, UV radiation with associated DNA damage, accumulation of reactive oxygen species (ROS), desiccation and osmotic stresses, it is important for organisms to accurately sense and respond to changes in light. The benefits of light are obvious for green organisms such as cyanobacteria, algae and plants which use light as an energy source (photosynthesis). Less apparent are other light-dependent processes such as light-driven DNA repair by photolyases (photoreactivation) or ion pumping by microbial opsins. Fungi that can share light-flooded habitats with phototrophs may profit from their excess photosynthetic products. Rock-inhabiting Dothideomycetes and Eurotiomycetes including Knufia petricola possess many proteins for absorbing UV/blue, green, red and far-red light, produce the black 1,8 dihydroxynaphthalene (DHN) melanin and orange-red carotenoids, and may live in multispecies biofilms. Here, we are addressing the question to which extent constitutive pigment formation (melanin and carotenoids) and responses mediated by the stress-activated mitogen-activated protein (MAP) kinase contribute to the observed light (UV-B) tolerance of K. petricola.
AbstractMicroplastics (MP) can be detected in all environmental systems. Marine and terrestrial aquatic systems, especially the transported suspended solids, have often been the focus of scientific investigations in the past. Sediments of aquatic river systems, on the other hand, were often ignored due to the time‐consuming sample preparation and analysis procedures. Spectroscopic measurement methods counting particle numbers are hardly suitable as detection methods, because there are plenty of natural particles next to a small number of MP particles. Integral methods, such as thermoanalytical methods are determining the particle mass independently of the inorganic components.In this study, a workflow for sample preparation via density separation and subsequent analysis by thermal extraction desorption‐gas chromatography/mass spectrometry is presented, which leads to representative and homogeneous samples and allows fast and robust MP mass content measurements suitable for routine analysis. Polymers were identified and quantified in all samples. Polyethylene and styrene‐butadiene rubber are the dominant polymers, besides polypropylene and polystyrene. Overall, total polymer masses between 1.18 and 337.0 µg/g could be determined. Highest MP concentrations in riverbed sediment are found in sites characterized by low flow velocities in harbors and reservoirs, while MP concentrations in sandy/gravelly bed sediments with higher flow velocities are small.
Emission testing of volatile organic compounds (VOC) from materials and products is commonly based on emission test chamber measurements. To ensure the comparability of results from different testing laboratories their measurement performance must be verified. For this purpose, Bundesanstalt für Materialforschung und -prüfung (BAM) organizes an international proficiency test every two years using well-characterised test materials (one sealant, one furniture board and four times a lacquer) with defined VOC emissions. The materials fulfilled the requirements of homogeneity, reproducibility, and stability. Altogether, 41 VOCs were included of which 37 gave test chamber air concentrations between 10 and 98 µg/m³. This is the typical concentration range to be expected and to be quantified when performing chamber tests. Four compounds had higher concentrations between 250 and 1105 µg/m³. The relative standard deviations (RSD) of BAM proficiency tests since 2008 are compared and the improvement of the comparability of the emission chamber testing is shown by the decrease of the mean RSD down to 23% in 2021. In contrast, the first large European interlaboratory comparison in 1999 showed a mean RSD of 51%.
Related work
Laboratory Study:
Combining Signal Features of Ground-Penetrating Radar to Classify Moisture Damage in Layered Building Floors
https://doi.org/10.3390/app11198820
On-Site Study:
TBA
Doctoral Thesis:
Non-destructive classification of moisture deterioration in layered building floors using ground penetrating radar
https://doi.org/10.14279/depositonce-19306
Measurement Parameters
The GPR measurements were carried out with the SIR 20 from GSSI and a 2 GHz antenna pair (bandwidth 1 GHz to 3 GHz) in common-offset configuration. Each B-Scan consists of N A-Scans, each including 512 samples of a 11 ns time window. Survey lines were recorded with 250 A-Scans/ meter, which equals a 4 mm spacing between each A-Scan No Gains were applied.
Folder Description:
Lab_dry, Lab_insulDamage, Lab_screedDamage
- each contain 168 Measurements (B-Scans) in .csv on 84 dry floors, floors with insulation damage and screed damage.
- each floor setup was measured twice on two orthogonal survey lines, indicated by _Line1_ and _Line2_ in the file name.
- the file names encode the building floor setup e.g. CT50XP100 describes a 50 mm cement screed with 100 mm extruded polystyrene below
- the material codes are
CT: cement screed, CA: anhydrite screed, EP: expanded polystyrene, XP: extruded polystyrene, GW: glass wool, PS: perlites
further information can be found in the publication https://doi.org/10.3390/app11198820
OnSite_
- 5 folders containing B-Scans on 5 different practical moisture damages
- the building floor setup is encoded according to the lab with an additional measurement point numbering at the start and a damage case annotation at the end of the file name with _dry, _insulationDamage and_screedDamage
File Description:
B-Scans, Measurement files - no header
- dimension: 512 x N data point with N beeing the number of A-Scans including 512 samples of a 11 ns time window.
- survey lines were recorded with 250 A-Scans/ meter, which equals a 4 mm spacing between each A-Scan
Moisture References
- Moist_Reference of On-Site Locations include the columns MeasPoint: Measurement point, wt%Screed: moisture content of screed layer in mass percent; wt%Insul: moisture content of insulation layer in mass percent. References were obtained by drilling cores with 68 mm diameter in the center of each survey line.
- Moist_Reference_Screed of Lab data include the columns Screed: Screed material and thickness in mm, wt%Screed moisture content of screed layer in mass percent
- Moist Reference_Insul of Lab data include the columns Insulation: Insulation material and thickness in mm, water addition in l: water added to the insulation layer in liters, V%Insulation: water added to the insulation layer in volume percent, RH%: resulting relative humidy in the insulation layer during measurement. These References are only avaible for Lab measurements on insulation damages.
By automatically recording as much information as possible in automated laboratory setups, reproducibility and traceability of experiments are vastly improved. This presentation shows what such an approach means for the quality of experiments in an X-ray scattering laboratory and an automated synthesis set-up.
Bacterial adhesion on surfaces of medical, water and food applications may lead to infections, water or food spoilage and human illness. In comparison to traditional static and macro flow chamber assays for biofilm formation studies, microfluidic chips allow in situ monitoring of biofilm formation under various flow regimes, have better environment control and smaller sample requirements.
In this work, a novel microfluidic platform is developed to investigate biofilm adhesion under precisely controlled bacteria concentration, temperature, and flow conditions. This platform central unit is a single-inlet microfluidic flow cell with a 5 mm wide chamber designed and tested to achieve ultra-homogenous flow in the central area of chamber. Within this area, defined microstructures are integrated that will disturb the homogeneity of the flow, thus changing bacterial adhesion pattern.
Here we present the monitoring of bacterial biofilm formation in a microfluidic chip equipped with a microstructure known as micro-trap. This feature is based on a 3D bacteria trap designed by Di Giacomo et al. and successfully used to sequester motile bacteria.
At first, fluorescent particles similar in size to Escherichia coli (E. coli) are used to simulate bacteria flow inside the flow cell and at the micro-trap. The turbulences induced by the trap are analyzed by imaging and particle tracking velocimetry (PTV). Secondly, the model strain E. coli TG1, ideal and well described for biofilm studies, is used to analyze biofilm formation in the micro-trap. Therefore, a stable fluorescent strain E. coli TG1-MRE-Tn7-141 is constructed by using Tn7 transposon mutagenesis according to the method described by Schlechter et al. Sequestering of E. coli cells within the micro-trap was followed using epifluorescence microscopy.
The novel microfluidic platform shows great potential for assessment of bacterial adhesion under various flow regimes. The performance of structural feature with respect to the generation of turbulences that promote or reduce bacterial adhesion can be systematically examined.
The combination of flow analysis and fluorescent strain injection into the microfluidic chip shows that the micro-trap is useful for capturing bacteria at defined positions and to study how flow conditions, especially micro-turbulences, can affect biofilm formation. It represents a powerful and versatile tool for studying the relation between topography and bacteria adhesion.
Nondestructive testing of gas turbine blades is essential for their maintenance and service process which is critical to ensure both safety and efficiency of these highly stressed parts. In this presentation, a novel ultrasonic testing method is explored in order to acquire part thickness information in the turbine blade’s airfoil. In established industry processes, the measurements are mainly carried out manually and only at a few specific positions of the inspected parts. The proposed method scans the part using a robot arm guiding an ultrasonic array sensor. For ultrasonic coupling to the complex-shaped surface geometry, the inspected part and sensor are immersed into water. A two-step TFM[1, 2] (Total Focusing Method) approach is used to reconstruct the outer and inner surfaces subsequently from the ultrasonic raw data, which are acquired using the FMC[3] (Full Matrix Capture) measurement principle. For each sensor position, the location and geometry of the outer surface is first identified and then used to create an image of an area inside the material. From that image, the inner surface is reconstructed. Finally, part thickness information is deducted from merging location data of inner and outer surface. The result is a high resolution, high precision mapping of the inspected part’s wall thickness.
Sunlight is an almost unavoidable environmental cue and plays a fundamental role in the biology of pro- and eukaryotic organisms. To cope with sunlight-associated stresses e.g., high temperatures, UV radiation, accumulation of reactive oxygen species, desiccation, and osmotic stress, it is important for organisms to accurately sense and respond to changes in light. The benefits of light are obvious for green organisms such as cyanobacteria, algae and plants which use light as an energy source (photosynthesis). Fungi that can share light-flooded habitats with phototrophs may profit from their excess photosynthetic products. Examples are the plant pathogen Botrytis cinerea, the gray mold fungus, and the rock inhabitant Knufia petricola, a microcolonial black fungus which forms multispecies biofilms with bacteria and algae.
Offshore wind energy plays an important role for the desired transition towards a carbon dioxide free industry within the next decades. However, the grounding of the offshore wind plants governs the overall installation process besides bureaucratic bottlenecks.
The application of lightweight principles in steel construction, e.g. the usage dissolved load-bearing structures, so called Jackets foundations, offer great potential in reducing the resource consumption, especially with respect to the needed amount of steel.
In this context this paper focuses on a fully digitalization of the welding manufacturing and as well as testing chain to enable a fully automated manufacturing as well as quality assessment of tubular nodes as key element of Jackets foundation structures. Furthermore, the relationship between the seam shape geometry and resulting fatigue strength is evaluated by numerical methods incorporating bionic principles. It is shown that tubular nodes can be welded fully automatically taking geometry tolerances into account. Moreover, the seam shape could be manufactured as requested by the numerical models which offers great potential for an extended lifetime. Subsequently, the improvements in resource efficiency and reduction of carbon dioxide emissions are evaluated by a life-cycle-assessment.
The European Commission adopted an EU
Energy Label for smartphones and tablets in June 2023. For the
first time, the EU Energy Label will depict, among energy
efficiency, also a reparability score, battery endurance, battery
lifetime, drop resistance and dust and water ingress protection.
However, does the multitude of parameters bear the risk to
confuse consumers instead of triggering environmentally
sustainable purchase decisions? In a survey in Germany,
consumers were asked to make a choice when the label does not
clearly identify the environmentally better product. Instead,
energy efficiency has to be valued against reparability,
reliability against energy efficiency, and reparability against
reliability. The sustainability lever of the different aspects is not
the same, as e.g. lifetime extension has a much more positive
environmental impact than energy efficiency in case of mobile
devices. Therefore, the question remains, whether consumers
intuitively make the right choice. Results of the survey indicate
that consumers interpret the complex label in the desired
manner and value those aspects higher, which indeed have a
more positive effect on the environment.
The overarching goal of this project is to develop a predictive model for efflux-mediated antimicrobial tolerance in bacterial multicellular assemblies. Our central hypostasis is that efflux pump activity causes emergent antibiotic tolerance of multicellular bacterial populations, through the interplay of efflux mediated spatial interactions and efflux-linked persistence. To test this hypothesis, we will use a combination of microscopy, microbial killing assays, computational modelling, and data analysis, integrating information from 3 types of multicellular assembly: colonies, cell-to-cell interactions in a monolayer microfluidic device, and 3D flow chamber biofilms. Building on our preliminary observations, we will experimentally characterize the link between colony structure and spatial patterns of efflux gene expression in strains that differ in their levels of efflux. We will develop a mathematical model to test whether local growth inhibition of neighbors due to effluxing cells, coupled with local environment-dependent regulation of efflux, can account qualitatively for these results. By including persister cell formation in our model we will predict, and measure, the emergent function of antimicrobial tolerance in our colonies. To fully understand how tolerance emerges from the interplay between efflux-mediated spatial interactions and efflux-linked persister cell formation, we need quantitative measurements at the single cell level. To this end, we will use a microfluidic setup with cells growing in a monolayer to qualify in detail the dependence of efflux expression and persister cell formation on nutrient conditions, the correlation between efflux and persister formation, and the spatial range of efflux-mediated neighbour growth inhibition. To predict and quantitatively understand the emergent multicellular function of tolerance, we will perform individual-based modelling of biofilm growth, using as input the parameters measured on the single-cell level with our microfluidics experiments. Our simulations will predict biofilm spatial structure development, patterns of efflux and persister formation and, ultimately, tolerance to antimicrobial challenge. These predictions will be directly tested in flow-cell biofilm experiments. We are currently generating acrAB-tolC knockout-strain, without efflux activity, and a strain with an inducible acrAB-tolC efflux pump. To distinguish the different strains under the microscope, they were labeled with genes encoding for different fluorescent proteins. All strains are currently characterized in terms of growth, minimum inhibitory concentration of different antimicrobial substances, colony morphology, and biofilm formation ability. On the theoretical side, we are currently working on modeling the system at various scales and degree of detail, ranging from coarse-grained continuum models to stochastic, individual-based models. Some exploratory work was doe to test existing software for individual-based modelling that may be adapted for our purpose. Furthermore, we are in the process of developing more coarse-grained models. This work involves some physiological modelling and literature search, focusing on working mechanisms of efflux pumps and kinetic models for import and export of antibiotics.
Consequences of tolerance to disinfectants on the evolution of antibiotic resistance in E. coli
(2023)
Biocides are used as disinfectants and preservatives; one important active substance in biocides is benzalkonium chloride (BAC). BAC-tolerant bacterial strains can survive short treatments with high concentrations of BAC. BAC tolerance and resistance have been linked to antibiotic resistance. Here, the selection dynamics between a BAC-tolerant Escherichia coli strain and a sensitive wild type were investigated under four conditions: in the absence of antibiotics and in the presence of three different sub-inhibitory concentrations of the antibiotic ciprofloxacin in liquid cultures. The wild type was selected over the BAC-tolerant strain in the absence of antibiotics, while the BAC-tolerant strain was selected over the wild type at all ciprofloxacin concentrations investigated, with a minimum selection concentration (MSC) of 1/10th of the minimum inhibitory concentration (MIC) of the wild type. Furthermore, the evolvability of resistance of the two strains to inhibitory concentrations of ciprofloxacin was assessed by performing a serial dilution evolution experiment with gradually increasing ciprofloxacin concentrations. The wild type had a higher probability to develop resistance
to ciprofloxacin than the tolerant strain. By the end of the evolution experiment both strains evolved to grow at the highest ciprofloxacin concentration investigated, which was 2048 ×MIC of the wild type. The importance of these results is highlighted by the fact that concentrations of ciprofloxacin well above the calculated MSC can be found in environmental samples such as hospital wastewaters and livestock slurry. In turn, BAC is used as a disinfectant in the same settings. Thus, the selection of BAC-tolerant strains at sub-inhibitory concentrations of ciprofloxacin can contribute to the stabilization and spread of BAC-tolerance in natural populations. The prevalence of such strains can impair the effects of BAC disinfections.
The contamination of drinking water with pharmaceuticals represents a severe health risk. In order to monitor the drinking water quality continuously and enable quick countermeasures in case of contamination, novel sensors are required. Here, immunoanalytical methods based on the binding of the analyte to highly selective antibodies can be helpful. In this work, magnetic bead-based immunoassays (MBBAs) have been developed for the detection of two relevant contaminants of drinking water: diclofenac (DCF) and amoxicillin (AMX). In case of the latter, not only the parent drug is of interest in the risk assessment but also its hydrolysis products (HPs). In a comprehensive study, the influence of external factors and intrinsic properties of the water on the rate of hydrolysis was investigated. As the hydrolysis of AMX further impacts the recognition by the antibody, a strategy to analyze samples with unknown hydrolysis degree of AMX was established employing the enzyme β-lactamase in sample preparation. For both analytes, the MBBAs enable the fast quantification with results obtained in less than one hour which represents a major improvement over conventional immunoassays like the enzyme-linked immunosorbent assay (ELISA). Compared to the respective ELISAs with the same antibodies, the MBBAs further exhibit improved analytical parameters such as a broader measurement range and lower limits of detection. Due to the magnetic properties of the beads that serve as a platform for the assays, they are suitable for the mobile and automated detection at the point-of-care. An integrated diagnostic system was designed in which electrochemical detection with chronoamperometry on a microfluidic chip allows for further miniaturization of the system to enable monitoring of the drinking water quality online in water supply pipes at waterworks.