Sanitär- und Kommunaltechnik; Umwelttechnik
Filtern
Erscheinungsjahr
- 2017 (1)
Dokumenttyp
- Zeitschriftenartikel (1) (entfernen)
Sprache
- Englisch (1)
Referierte Publikation
- ja (1)
Schlagworte
We report for the first time the formation of sitespecific interstrand cross-linked (ICL) surface-immobilized furanmodified DNA duplexes via singlet oxygen. 1O2, necessary for effecting furan-mediated ICL formation, was produced in situ using methylene blue or a zinc phthalocyanine derivative (TT1) as a photosensitizer. Via surface plasmon resonance spectroscopy, we show that surface ICL was achieved, and a robust link formed that enhances the stability of the 12-mer duplex even after surface regeneration. The described method represents a novel platform technology based on surfaces with addressable and stable DNA duplexes requiring only short oligonucleotides.