Ingenieurwissenschaften und zugeordnete Tätigkeiten
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- Photoluminescence quantum yield (1) (entfernen)
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An increasing number of (bio)analytical techniques rely on multiparametric analyses and the measurement of a very small number of emitters. While the former implies encoding or labeling by means of easily distinguishable properties like luminescence color or lifetime in conjunction with high-throughput optical-spectroscopic methods such as flow cytometry, the latter requires methods suitable for the characterization of the optical properties of single emitters. Here, we present the use of fluorescence correlation spectroscopy (FCS) for the relative determination of the key parameter photoluminescence quantum yield [5] and first results from flow cytometry measurements in the time-domain with a custom-designed instrument with luminescence lifetime analysis capability.