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Trends in fluorometry and fluorescence imaging are increasing applications of molecular and nanoscale reporters with emission > 800 nm and recently also > 1000 nm for bioanalysis, medical diagnostics, bioimaging, and safety barcodes. Mandatory for the comparison of different emitter classes and the rational design of the next generation of reporters for the short wavelength infrared (SWIR) region are reliable and quantitative photoluminescence measurements in this challenging wavelength region. This is of special relevance for nanocrystalline emitters like semiconductor quantum dots and rods as well as lanthanide-based upconversion and downconversion nanocrystals, where surface states and the accessibility of emissive states by quenchers largely control accomplishable photoluminescence quantum yields and hence, signal sizes and detection sensitivities from the reporter side. Such measurements are currently hampered by the lack of suitable methods and standards for instrument calibration and validation and quantum yield standards with emission > 800 nm and especially > 1000 nm.
In this respect, we present the design of integrating sphere setups for absolute and excitation power density-dependent measurements of emission spectra and photoluminescence quantum yields in the wavelength region of 650 to 1650 nm including calibration strategies and first candidates for potential fluorescence standards. Subsequently, selected examples for spectroscopic studies of different types of nanocrystals are presented including the upconversion and downconversion emission of differently sized and surface functionalized lanthanide-doped nanoparticles
There is an increasing interest in optical reporters like semiconductor quantum dots and upconversion nanophosphors with emission > 1000 nm for bioanalysis, medical diagnostics, and safety barcodes and hence, in reliable fluorescence measurements in this wavelength region, e.g., for the comparison of material performance and the rational design of new nanomaterials with improved properties. Here, we present the design of an integrating sphere setup for the absolute measurement of emission spectra and quantum yields in the wavelength region of 650 to 1600 nm and its calibration as well as examples for potential fluorescence standards from different reporter classes for the control of the reliability of such measurements.