Ingenieurwissenschaften und zugeordnete Tätigkeiten
Filtern
Erscheinungsjahr
- 2017 (2) (entfernen)
Dokumenttyp
- Vortrag (2)
Sprache
- Englisch (2)
Referierte Publikation
- nein (2) (entfernen)
Schlagworte
- Dye (2) (entfernen)
Eingeladener Vortrag
- nein (2)
Mandatory for the comparison of different emitter classes and the rational design of the next generation of molecular and nanoscale reporters are reliable and quantitative photoluminescence measurements. This is of special relevance for all fluorescence applications in the life and material sciences. In the following, proccedures for the determination of this spectroscopic key parameter are presented including material-specific effects related to certain emitters
Comparison of fluorescence measurements performed on different fluorescence instruments, analyte quantification from fluorescence intensities as well as the determination of fluorescence quantum yields require instrument calibration and consideration of the wavelength-dependent instrument-specific quantities spectral photon flux reaching the sample and spectral responsivity. Here, we present guidelines and recommendations for the qualification of fluorescence instruments and introduce suitable chromophore-based reference materials. Moreover, the design concepts of the different BAM fluorescence standards are discussed.