Ingenieurwissenschaften und zugeordnete Tätigkeiten
Filtern
Dokumenttyp
- Posterpräsentation (2) (entfernen)
Sprache
- Englisch (2)
Referierte Publikation
- nein (2)
Schlagworte
- Affinity chromatography (1)
- Affinity extraction (1)
- Affinity support (1)
- Aluminum oxide (1)
- Amino acid analysis (1)
- Amplification (1)
- Antibodies (1)
- Antibody purification (1)
- Aromatic amino acid analysis AAAA (1)
- BSA (1)
- Bovine serum albumin (1)
- Data analysis (1)
- Downstream processing (1)
- ESEM (1)
- Fluorescence (1)
- Glue (1)
- Glutaraldehyde (1)
- Human plasma (1)
- Illumina (1)
- PEG (1)
- Phosphonic acids (1)
- Polyglycerol (1)
- Polymer (1)
- Protein a (1)
- Protein hydrolysis (1)
- Protein immobilization (1)
- Protein quantification (1)
- Reductive amination (1)
- SALSA (1)
- SDS-PAGE (1)
- Sanger sequencing (1)
- Sapphire (1)
- TEM (1)
- Tyrosine (1)
Organisationseinheit der BAM
- 1.5 Proteinanalytik (2) (entfernen)
Nonporous corundum powder was functionalized covalently with protein binders to isolate and enrich specific proteins from complex matrices.The common crosslinker glutaraldehyde was compared with a hyperbranched polyglycerol (PG) of around 10 kDa. The latter was oxidized with periodate to generate aldehyde groups that can covalently react with the amines of the surface and the amino groups from the protein via a reductive amination process. The amount of bound protein was quantified via aromatic amino acid analysis (AAAA). As a proof of concept, IgG was extracted with protein A from crude human plasma. The advantages of corundum include the very low price, extremely high physical and chemical stability, pressure resistance, favorable binding kinetics, convenient handling, and flexible application.
Phage display is used to find specific target binding peptides for polypropylene (PP) surfaces. PP is one of the most commonly used plastics in the world. Millions of tons are produced every year. PP binders are of particular interest because so far gluing or printing on PP is challenging due to its low surface energy. A phage display protocol for PP was developed followed by Next Generation DNA Sequencing of the whole phage library. Data analysis of millions of sequences yields promising peptide candidates which were synthesized as PEG conjugates. Fluorescence-based adsorption-elution-experiments show high adsorption on PP for several sequences.