Ingenieurwissenschaften und zugeordnete Tätigkeiten
Filtern
Dokumenttyp
Schlagworte
- Quantum yield (75)
- Fluorescence (74)
- Nano (60)
- Lifetime (54)
- Dye (48)
- Photophysics (41)
- Quality assurance (41)
- Sensor (38)
- Photoluminescence (37)
- NIR (34)
- Lanthanide (33)
- Nanoparticle (32)
- Particle (32)
- Nanomaterial (30)
- Upconversion (26)
- Method (25)
- Surface chemistry (22)
- Mechanism (20)
- Synthesis (19)
- SWIR (18)
- Upconversion nanoparticle (18)
- Energy transfer (15)
- Quantum dot (15)
- Reference material (15)
- Quantum dots (14)
- Integrating sphere spectroscopy (13)
- Brightness (12)
- Luminescence (11)
- Multiplexing (11)
- Optical spectroscopy (11)
- Quantification (11)
- Quantitative spectroscopy (10)
- Single particle (10)
- Imaging (9)
- Nanoparticles (9)
- Aggregation (8)
- Assay (8)
- Bead (8)
- Electron microscopy (7)
- Flow cytometry (7)
- Polymer (7)
- Semiconductor quantum dot (7)
- Size (7)
- AIS (6)
- Barcoding (6)
- Calibration (6)
- Coating (6)
- Crystal (6)
- Encoding (6)
- Excitation power density (6)
- Life sciences (6)
- Ligand (6)
- Microscopy (6)
- Optical probe (6)
- Surface group analysis (6)
- Uncertainty (6)
- Application (5)
- Bioimaging (5)
- Cell (5)
- IR (5)
- Measurement uncertainty (5)
- Modeling (5)
- Nanocrystal (5)
- Rare earth nanoparticles (5)
- SAXS (5)
- Semiconductor (5)
- Signal enhancement (5)
- Silica (5)
- Single particle spectroscopy (5)
- Stability (5)
- Standard (5)
- Surface (5)
- Surface analysis (5)
- Switch (5)
- Temperature (5)
- Absolute fluorometry (4)
- Aging (4)
- Electrochemistry (4)
- Environment (4)
- Fluorescence standard (4)
- Image segmentation (4)
- InP (4)
- Iron oxide nanoparticles (4)
- PDT (4)
- Probe (4)
- Reference materials (4)
- Spectroscopy (4)
- TEM (4)
- Traceability (4)
- pH (4)
- AIE (3)
- Active fibers (3)
- Advanced Materials (3)
- Aggregation-induced emission (3)
- Antibody (3)
- BODIPY (3)
- Certification (3)
- Cleavable probe (3)
- Cr(III) (3)
- Cytotoxicity (3)
- Enhancement (3)
- Extracellular vesicles (EV) (3)
- Flourescence (3)
- Fluoride (3)
- Gold nanocluster (3)
- ICP-OES (3)
- In vivo imaging (3)
- Indium phosphide (3)
- Label (3)
- Limit of detection (3)
- Linearity (3)
- Method development (3)
- Modelling (3)
- Nano particle (3)
- Optical assay (3)
- Optical assays (3)
- Particle Synthesis (3)
- Polarity (3)
- Quantitative NMR (3)
- Ratiometric sensors (3)
- Reference data (3)
- Reference product (3)
- Reporter (3)
- Safe-by-Design (3)
- Sensitization (3)
- Sensors (3)
- Solid state emission (3)
- Surface group quantification (3)
- Upconversion nanoparticles (3)
- Upconverting nanoparticles (3)
- XPS (3)
- Yb(III) complex (3)
- pH probe (3)
- pH sensing (3)
- 150th anniversary (2)
- ABC (2)
- Absolute fluorescence (2)
- Absolute fluorescence quantum yield (2)
- Advanced nanomaterials (2)
- Aggregation induced emission (2)
- Amorphous silica particles (2)
- Analysis (2)
- Analytical sciences (2)
- Automated image analysis (2)
- Automatisation (2)
- BAM (2)
- Bead-based assay (2)
- Bundesanstalt für Materialforschung und -prüfung (2)
- Cancer (2)
- Carbon dot (2)
- Cathodoluminescence (2)
- Cell studies (2)
- Characterization (2)
- Collection (2)
- Conjugate (2)
- Contrast agent (2)
- Convolutional neural networks (2)
- Copolymer (2)
- Core-shell nanoparticles (2)
- Deactivation pathways (2)
- EMPIR 18HLT01 MetVesII (2)
- Engineered Nanomaterials (2)
- Enhancement strategy (2)
- Excitation (2)
- Exciton diffusion (2)
- FLIM (2)
- FRET (2)
- Flow cytometry (FCM) (2)
- Fluorescence decay kinetics (2)
- Fluorescent particles (2)
- Funtional Groups (2)
- Gold (2)
- Instrument calibration (2)
- Interlaboratory comparison (2)
- Iron oxide (2)
- Jahrestag (2)
- Ligands (2)
- Llifetime (2)
- Magic-sized cluster (2)
- Mass spectrometry (2)
- Material sciences (2)
- Metal cluster (2)
- Method comparison (2)
- Method validation (2)
- Microparticle (2)
- Monitoring (2)
- Monomer (2)
- NIR-II (2)
- NMR (2)
- Nano- and microsensors (2)
- Nanomaterialien (2)
- Nanosafety (2)
- Nanosensor (2)
- Nanotoxicity (2)
- Neural networks (2)
- Optical Spectroscopy (2)
- Particle size (2)
- Particle surface analysis (2)
- Particle synthesis (2)
- Performance validation (2)
- Perovskite (2)
- Photoluminescence quantum yield (2)
- Photonic crystal (2)
- Pollutant (2)
- Polymer particle (2)
- Power density (2)
- Protein (2)
- Quantitative Analysis (2)
- Quantum Yield (2)
- Quantum yields (2)
- Quenching (2)
- Reliability (2)
- Safety (2)
- Semiconductor nanocrystal (2)
- Semiconductor nanocrystals (2)
- Sensing (2)
- Sensor molecules (2)
- Shape (2)
- Shell (2)
- Silica and Polystyrene Particles (2)
- Silica and polystyrene nanoparticles (2)
- Simulation (2)
- Singlet oxygen (2)
- Special issue (2)
- Standardization (2)
- Surface Group Analysis (2)
- Sythesis (2)
- Ternary quantum dots (2)
- Titration (2)
- Triplet-triplet annihilation (2)
- Upconversion nanocrystal (2)
- ZnSe (2)
- fluorescence (2)
- 18HLT01 MetVes II (1)
- 2D materials (1)
- A4F (1)
- AIS QD (1)
- ASAXS (1)
- Ab initio calculation (1)
- Absolute (1)
- Absolute measurement (1)
- Absorption (1)
- Advanced material (1)
- Advanced materials (1)
- Ag2S (1)
- Amplification (1)
- Analytical services (1)
- Antibody conjuagtes (1)
- Antibody conjugates (1)
- Aqueous quantum dot (1)
- Artificial Intelligence (1)
- Artificial intelligence (1)
- Artificial weathering (1)
- Au nanodisks (1)
- Au nanoparticles (1)
- Automated Image Analysis (1)
- Automated assay (1)
- Automated synthesis (1)
- Automation (1)
- Aza-BODIPY (1)
- Bacteria (1)
- Bacterial lipopolysaccharides (1)
- Bioconjugate (1)
- Biofilm (1)
- Biomimicry (1)
- Biosensing (1)
- Boron Neutron Capture Therapy (BNCT) (1)
- Brownsche Molekularbewegung (1)
- Bundesoberbehörden (1)
- C-dot (1)
- COSMO (1)
- CRP (1)
- CUINS2 nanocrystals (1)
- Calibrated fluorescence measurements (1)
- Capillary electrophoresis (1)
- Carboxyl group (1)
- Catalogue of services (1)
- Catch and release assay (1)
- Cement (1)
- Cement hydration (1)
- Certified reference material (1)
- Charge transfer (1)
- Chromium (III) complexes (1)
- Cleavable linker (1)
- Cleavable probes (1)
- Cluster (1)
- Colloidal semiconductor nanocrystals (1)
- Color (1)
- Color tuning (1)
- Comparison (1)
- Complex (1)
- Conductometry (1)
- Conductor-like screening model (1)
- Converter material (1)
- Core shell structure (1)
- Core/shell materials (1)
- Core/shell particle (1)
- Core/shell quantum dot (1)
- Corrosion (1)
- Cr(III) complex (1)
- Crosslinking (1)
- Cubical shape (1)
- Cyanine (1)
- DNA (1)
- DNA origami (1)
- DPA (1)
- Decay kinetics (1)
- Defect photoluminescence (1)
- Degradation (1)
- Design of experiment (1)
- Dipole moment (1)
- Dodecanethiol (1)
- Doping (1)
- Dual sensing (1)
- Dyad molecules (1)
- Dye labeling (1)
- Dyes (1)
- EC4SafeNano (1)
- EMPIR project (1)
- Editorial (1)
- Electrochemical Titration (1)
- Electrochemical titration (1)
- Electron Microscopy (1)
- Emission enhancement (1)
- Engineered nanomaterials (1)
- Er(III) (1)
- European Centre (1)
- Extracellualr vesicles (1)
- FTIR (1)
- Field sensor (1)
- Fluoranthene (1)
- Fluorescence intensity ratio (1)
- Fluorescence lifetime (1)
- Fluorescence microscopy (1)
- Fluorescence quantum yield (1)
- Fluorescence spectroscopy (1)
- Fluorescent dye (1)
- Fluorescent glasses (1)
- Fluorescent indicator (1)
- Fluorescent label (1)
- Fluorescent probe (1)
- Fluorophore (1)
- Forschungsstrategie (1)
- Fully aromatic frameworks (1)
- Functional group analysis (1)
- Functional group quantification (1)
- Functionalized Nanomaterials (1)
- Functionalized nano- and microparticles (1)
- Functionalized silica and polymeric particles (1)
- Gallium doping (1)
- Gold nanoclusters (1)
- Green synthesis (1)
- Heory (1)
- High-resolution transmission electron microscopy (1)
- Homogeneity (1)
- Hydration (1)
- IR spectroscopy; conductometry (1)
- Image Segmentation (1)
- Imaging techniques (1)
- Immunoassay (1)
- Infrastructure (1)
- Integrating sphere spectroscopy, (1)
- Interlabority comparison (1)
- K+ doped (1)
- LT-FCM (1)
- Lanthanide-doped nayf-4 (1)
- Lanthanides (1)
- Lead-free (1)
- Legionella (1)
- LiYF4 (1)
- Lichtstreuung (1)
- Lifetime analysis (1)
- Ligand exchange (1)
- Ligand quantification (1)
- Light harvesting (1)
- Luminescence lifetime measurments (1)
- Luorescence (1)
- MIC (1)
- MOF (1)
- MS (1)
- Magnetic (1)
- Medicine (1)
- Merocyanine (1)
- Metal (1)
- Metal-organic frameworks (1)
- Metasurface (1)
- Methos comparision (1)
- Microfluidics (1)
- Microorganism (1)
- Microparticles (1)
- Mie resonances (1)
- Moisture (1)
- Multicolored (1)
- Multifunctional efficiency (1)
- NIR dyes (1)
- NIR-II Imaging (1)
- NIR-II fluorescence (1)
- NIRII (1)
- Nanaoparticle (1)
- Nano CRM (1)
- Nano-safety (1)
- Nanocrystals (1)
- Nanomaterial design (1)
- Nanomaterials (1)
- Nanomedicine (1)
- Nanoparticle characterization (1)
- Nanopartikel (1)
- Nanoplatelet (1)
- Nanoplattform (1)
- Nanosafety (Safe-by-design) (1)
- Nanoscale luminescent reporters (1)
- Nanosensors (1)
- Nanotechnologie (1)
- Nd excitation (1)
- Near infrared (1)
- Near-infrared light (1)
- Neuartige Materialien (1)
- Neural Networks (1)
- Nicht lineare Prozesse (1)
- Nile Red (1)
- Nnano particle (1)
- Non lienear processes (1)
- Non-thermally coupled levels (1)
- One-pot synthesis (1)
- Optical Assays (1)
- Optical detection (1)
- Optical probes (1)
- Optical properties (1)
- Optical spectroscopie (1)
- Optical temperature sensing (1)
- Organic–inorganic nanostructures (1)
- Origami (1)
- Ovarian cancer (1)
- Oxygen (1)
- Oxygen sensing (1)
- Oxygen sensitive (1)
- PEG (1)
- PEG ligands (1)
- PH (1)
- PH probe (1)
- Particle architecture (1)
- Particle sensors (1)
- Particle, imaging (1)
- Partikelgrößenbestimmung (1)
- Partikelkonzentration (1)
- Passivation shell (1)
- Pd(II) (1)
- Perovskites (1)
- Ph (1)
- Phosphinine (1)
- Phosphor (1)
- Photocatalytic activity (1)
- Photon bunching (1)
- Photophysic (1)
- Photosensitizer (1)
- Photostability (1)
- Polymer particles (1)
- Polymers (1)
- Polystyrene microparticles (1)
- Polyurethane (1)
- Porosity (1)
- Porphyrin (1)
- Power density dependence (1)
- Pphotophysics (1)
- Production (1)
- Pseudomonas aeruginosa (1)
- Pt(II) (1)
- QNMR (1)
- Qantum yield (1)
- Quality assurcance (1)
- Quantenausbeute (1)
- Quantum Dot-Rod (1)
- Quantum Yields (1)
- Quantum chemistry (1)
- Quantum do (1)
- Quantum dot-rod (1)
- Quantum rod (1)
- Quinones (1)
- Ratiometric (1)
- Ratiometric Sensors (1)
- Ratiometric sensing (1)
- Redox (1)
- Reference methods (1)
- Reference nanomaterials (1)
- Reference nanoparticles (1)
- Reference particles (1)
- Reflection spectroscopy (1)
- Release (1)
- Reliable characterization (1)
- Renewable energy (1)
- Reproducibility (1)
- Reverse microemulsion (1)
- Risk assessment (1)
- Rreference material (1)
- SWIR photoluminescence (1)
- Saccharide sensing (1)
- Safer by design (1)
- Scattering (1)
- Self-assembly (1)
- Semiconductur (1)
- Shortwave infrared (1)
- Silanization (1)
- Silica Nanoparticles (1)
- Silica coating (1)
- Silica- and Polystyrene Particles (1)
- Silica- and polystyrene particles (1)
- Silicon nanoparticles (1)
- Silver indium sulfide (1)
- Single emitter (1)
- Single enhancement (1)
- Single molecule (1)
- Single-dot spectroscopy (1)
- Size and size distribution (1)
- Small angle x-ray scattering (1)
- Small-angle scattering (1)
- Solar cell (1)
- Solar cells (1)
- Solar concentrator (1)
- Solar energy (1)
- Solid (1)
- Spectroscopy / Instrumentation (1)
- Spectroscopy / Theory (1)
- SrF2 (1)
- Standardisierung (1)
- Standards (1)
- Stepwise growth (1)
- Surface Chemistry (1)
- Surface Modification (1)
- Surface coating (1)
- Surface functional group quantification (1)
- Surface group (1)
- Surface groups (1)
- Surface ligand (1)
- Surface modification (1)
- Surface modified nano- and microparticles (1)
- Sustainable-by-Design (1)
- Suzuki-Miyaura coupling (1)
- Swell-capture (1)
- TMDCs (1)
- Tag (1)
- Terminal functional groups (1)
- Theory (1)
- Theranostics (1)
- Therapy (1)
- Thermal coupling energy level (1)
- Thermally coupled levels (1)
- Thick shells (1)
- Thickness (1)
- Thiols (1)
- Time-gated emission (1)
- Time-resolved flow cytometry (1)
- Toxicity (1)
- Transition metal dichalcogenide (1)
- Transmission electron microscopy (1)
- Triggered (1)
- UV Vis (1)
- UpConversion (1)
- Upconverion (1)
- Upconversion luminescence (1)
- Upconverstion (1)
- VAMAS (1)
- X-ray (1)
- X-ray Photoelectron Spectroscopy (1)
- X-ray microspectroscopy (1)
- X-ray spectroscopy (1)
- aggregation-induced dual emission (AIDE) (1)
- anion-exchange (1)
- antibody (1)
- bacteria (1)
- bacteria detection (1)
- bioimaging (1)
- calibration (1)
- dye (1)
- flow cytometry (1)
- fluorescence microscopy (1)
- fluorescence standards (1)
- immunoseparation (1)
- lifetime (1)
- magnetic nanoparticle (1)
- method (1)
- nanoparticle (1)
- organic dyes (1)
- particle (1)
- photoluminescence (1)
- photophysics (1)
- quality assurcance (1)
- quantum dot (1)
- quantum yield (1)
- screening tes (1)
- surface group analysis (1)
- synthesis (1)
Organisationseinheit der BAM
- 1.2 Biophotonik (212) (entfernen)
Paper des Monats
- ja (7)
Eingeladener Vortrag
- nein (62)
The overall interest in nanotoxicity, triggered by the increasing use of nanomaterials in the material and life sciences, and the synthesis of an ever increasing number of new functional nanoparticles calls for standardized test procedures1,2 and for efficient approaches to screen the potential genotoxicity of these materials. Aiming at the development of fast and easy to use, automated microscopic methods for the determination of the genotoxicity of different types of nanoparticles, we assess the potential of the fluorometric γH2AX assay for this purpose. This assay, which can be run on an automated microscopic detection system, relies on the detection of DNA double strand breaks as a sign for genotoxicity3. Here, we provide first results obtained with broadly used nanomaterials like CdSe/CdS and InP/ZnS quantum dots as well as iron oxide, gold, and polymer particles of different surface chemistry with previously tested colloidal stability and different cell lines like Hep-2 and 8E11 cells, which reveal a dependence of the genotoxicity on the chemical composition as well as the surface chemistry of these nanomaterials. These studies will be also used to establish nanomaterials as positive and negative genotoxicity controls or standards for assay performance validation for users of this fluorometric genotoxicity assay. In the future, after proper validation, this microscopic platform technology will be expanded to other typical toxicity assays.
Lanthanide-based upconversion nanoparticles (UCNPs) offer new strategies for luminescence-based sensing and imaging. One of the best studied materials are hexagonal ß-NaYF4 UCNPs doped with 20% Yb3+ and 2% Er3+, which efficiently convert 976 nm light to photons emitted at 540 nm, 655 nm, and 845 nm, respectively, reveal Long luminescence lifetimes (> 100 µs), and are very photostable and chemically inters.[1,2] The properties of their upconversion (UC) luminescence (UCL) are, however, strongly influenced by particle size, concentration and spatial arrangement of dopant Ions, surface chemistry, and microenvironment.[3,4] In addition, the multiphotonic absorption processes responsible for UCL render UCL dependent on excitation power density (P).
The rational design of brighter UCNPs particle architectures encouraged us to assess systematically the influence of these parameters on UCL for differently doped UCNPs relying on the commonly used ß-NaYf4 matrix using steady state and time resolved fluorometry as well as integrating sphere spectroscopy for P varied over almost three orders of magnitude. This includes comprehensive studies of the influence of size and shell, Yb3+ and Er3+ dopand concentrations, and energy Transfer processes from UCNPs to surface-bound organic dyes or vice versa [5]. Our results underline the need for really quantitative luminescence studies for mechanistic insights, the potential of high p to compensate for UCL surface quenching, and the matrix- and P-dependence of the optimum dopand concentration.