Analytische Chemie
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- 2023 (1)
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The poster describes how molecular biology, especially recombinant expression of proteins, in this case, an enzyme, can underpin developments of biosensors. The fumonisin oxidase produced by the fungus Aspergillus niger (AnFAO) is highly selective for the toxic mycotoxin fumonisin. Its structure and sequence has been published before. We took this information and expressed the enzyme in E. coli. The enzyme proved active and could be employed in an amperometric biosensor for the detection of the mycotoxin.