Datei im Netzwerk der BAM verfügbar ("Closed Access")
Filtern
Erscheinungsjahr
- 2016 (3) (entfernen)
Dokumenttyp
- Posterpräsentation (2)
- Vortrag (1)
Sprache
- Englisch (3)
Referierte Publikation
- nein (3)
Schlagworte
Eingeladener Vortrag
- nein (1)
The performance of a miniaturized channel for the separation of polymer and metal nanoparticles (NP) using Asymmetrical Flow Field-Flow Fractionation (AF4) was investigated and compared with a conventional AF4 system. To develop standard separation methods, experimental parameters like cross flow, gradient profile and injection time were varied and optimized. Corresponding chromatographic parameters were calculated and compared. Our results indicate that the chromatographic resolution in the miniaturized channel is lower, whereas significantly shorter analyses time and less solvent consumption were obtained. Moreover, the limit of detection (LOD) and limit of quantification (LOQ) obtained from hyphenation with a UV-detector are obviously lower than in a conventional channel, which makes the miniaturized channel interesting for trace analysis.
Currently available separation channels for asymmetric flow
field-flow fractionation (AF4), which results in long analysis
time and solvent consumption, limits the application of AF4 in
[1} the field of nanoparticle analysis .
A miniaturized AF4 channel was introduced for the rapid
analysis of different nanoparticle samples. UV-Vis-MALS
provides the possibility to measure the particle size and molar
mass and offers a powerful tool for the investigation of
[2] separation performances of different AF4 channels .
Our objective was to develop fast and reliable separation
methods for both channels and enable a direct comparison by
the corresponding chromatographic parameters.