Chemie und Prozesstechnik
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Erscheinungsjahr
- 2019 (22) (entfernen)
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- Zeitschriftenartikel (22) (entfernen)
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- ICP-MS (7)
- Nanoparticle (6)
- Cell (5)
- Laser ablation (5)
- Delta value (2)
- Imaging (2)
- Isotope ratio (2)
- Mass spectrometry (2)
- Measurement uncertainty (2)
- Antifouling biocides (1)
Organisationseinheit der BAM
- 1.1 Anorganische Spurenanalytik (22) (entfernen)
Recent recommendations by the Food and Drug Administration1 and the European Medicines Agency2 are to limit the clinical use of linear gadolinium-based contrast agents (GBCAs) due to convincing evidence of deposition in tissues. Macrocyclic GBCA continued to be considered safe, provided that patients have normal renal function. To date, given the low sensitivity of conventional MRI, there has been a debate about the signal increase following the injections of a macrocyclic GBCA.
In this paper, we describe the labelling of antibodies by gold nanoparticles (AuNPs) with diameters of 10 and 60 nm with detection by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). Additionally, the AuNPs labelling strategy is compared with commercially available labelling reagents based on MeCAT (metal coded affinity tagging). Proof of principle experiments based on dot blot experiments were performed. The two labelling methods investigated were compared by sensitivity and limit of detection (LOD). The absolute LODs achieved were in the range of tens of picograms for AuNP labelling compared to a few hundred picograms by the MeCAT labelling.