4 Material und Umwelt
Filtern
Dokumenttyp
- Vortrag (91)
- Zeitschriftenartikel (90)
- Posterpräsentation (61)
- Beitrag zu einem Tagungsband (11)
- Forschungsbericht (4)
- Buchkapitel (3)
- Dissertation (2)
- Sonstiges (2)
- Beitrag zu einem Sammelband (1)
- Video (1)
Sprache
- Englisch (233)
- Deutsch (31)
- Chinesisch (2)
Schlagworte
- Biocides (44)
- Antimicrobial resistance (34)
- MIC (23)
- Bacteria (22)
- Biofilms (22)
- Corrosion (20)
- Evolution (13)
- Methanogens (13)
- Microbiologically influenced corrosion (13)
- Biofilm (11)
- Disinfection (11)
- E. coli (11)
- NAP-XPS (11)
- Antimicrobial coating (10)
- Archaea (8)
- Biocide (8)
- Ectoine (8)
- Microbiology (8)
- Persistence (8)
- Resistance (8)
- Standardization (8)
- Horizontal gene transfer HGT (7)
- Laser-induced periodic surface structures (LIPSS) (7)
- Resistance evolution (7)
- Antibiotics (6)
- Bacterial adhesion (6)
- Biocide tolerance (6)
- Korrosion (6)
- Termites (6)
- Tolerance (6)
- Wood protection (6)
- Alginate (5)
- Cross-resistance (5)
- Environmental Simulation (5)
- Heterogeneity (5)
- Horizontal gene transfer (5)
- Methanogen (5)
- Mutation rate (5)
- Risk assessment (5)
- Sc-ICP-ToF-MS (5)
- Single cell analysis (5)
- Umweltsimulation (5)
- XPS (5)
- Anobium punctatum (4)
- Antimicrobials (4)
- Biocorrosion (4)
- Biofilm formation (4)
- Carbon steel (4)
- Corrosion products (4)
- Glutaraldehyde (4)
- Hi-Tension (4)
- Holzschutz (4)
- Kulturgut (4)
- Method development (4)
- NanoSIMS (4)
- Polyethylene (4)
- Tineola bisselliella (4)
- Anoxia (3)
- Antimicrobial (3)
- Antimicrobial surfaces (3)
- Antimikrobielle Resistenz (3)
- Antmikrobielle Oberflächen (3)
- Biozide (3)
- Carbon steel corrosion (3)
- DIN (3)
- Environmental simulation (3)
- FIB/SEM (3)
- Flow Model (3)
- Halomonas elongata (3)
- Immunity (3)
- Ir DNA staining approach (3)
- LIPSS (3)
- Laser processing (3)
- Leaching (3)
- Metabolism (3)
- Methanogen-induced microbiologically influenced corrosion (3)
- Microbiological influenced corrosion MIC (3)
- Mikrobiell beeinflusste Korrosion (3)
- Modelling (3)
- Modelling studies (3)
- Nanoparticles (3)
- Normung (3)
- Osmoadaptation (3)
- Phenotypic diversity (3)
- SC-ICP-ToF-MS (3)
- Single cell (3)
- Standardisierung (3)
- Sulfate reducing bacteria (3)
- Termite (3)
- Wastewater (3)
- Wood Protection (3)
- Antagonism (2)
- Antibiotic resisitance (2)
- Apanteles carpatus (2)
- Bacterial adhesion tests (2)
- Bacterial attachment (2)
- Baryscapus tineivorus (2)
- Biodiversity (2)
- Biofilm growth (2)
- Bioinformatics (2)
- Biological pest control (2)
- Biotechnology (2)
- Brown-rot fungi (2)
- CEN (2)
- Compatible solute (2)
- Condensed Matter Physics (2)
- Corrosion evolution (2)
- Corynetes caeruleus (2)
- Cultural heritage (2)
- DNA (2)
- Degradation (2)
- Desinfectant (2)
- Disinfectants (2)
- Disposal repository (2)
- E.coli (2)
- Energy Engineering and Power Technology (2)
- Environmental condition (2)
- Flow system (2)
- Fuel Technology (2)
- HAXPES (2)
- HI-Tension (2)
- Halomonas CRISPR Ectoine (2)
- Halophile (2)
- IPM (2)
- ISO (2)
- Insect biotechnology (2)
- Iron (2)
- Isoptera (2)
- Keratin (2)
- Korynetes caeruleus (2)
- LA-ICP-MS (2)
- Life history data (2)
- Microorganism (2)
- Mikroorganismen (2)
- Model (2)
- Nitrogen (2)
- Nosema ceranae (2)
- Nuclear waste (2)
- Optics (2)
- Osmia bicornis (2)
- Persister cells (2)
- Pollutants (2)
- Pseudomonas aeruginosa (2)
- Reactive oxygen species (2)
- Renewable Energy, Sustainability and the Environment (2)
- Risk assesment (2)
- Schadstoffaustrag (2)
- Schimmel (2)
- Shale (2)
- Social (2)
- UV-irradiation (2)
- Wild bees (2)
- 2D nanomaterials (1)
- 3D printing (1)
- A-DNA conformation (1)
- AMR (1)
- Academia (1)
- Acidobacteria (1)
- Agarose (1)
- Alkylating agents (1)
- Amphiphilic polymer (1)
- Anaerobic pathways (1)
- Analytical standards (1)
- Anpassung (1)
- Anti-microbial effects (1)
- Antiadhesive surfaces (1)
- Antibacterial (1)
- Antibacterial polymer (1)
- Antibacterial surfaces (1)
- Antibakterielle Oberflächen (1)
- Antibiotic (1)
- Antifouling (1)
- Antimicrobial Resistance (1)
- Antimicrobial test (1)
- Antiseptic agents (1)
- Artificial weathering (1)
- Aspergillus (1)
- Aspergillus flavus (1)
- Atomic and Molecular Physics and Optics (1)
- Atomic force microscopy (1)
- Auslaugtests (1)
- Automated analysis (1)
- BODIPY (1)
- Bacillus subtilis (1)
- Bacteria repellent surfaces (1)
- Bacteria-repellent surfaces (1)
- Bacterial cell cycle (1)
- Bacterial growth (1)
- Bacterial second messenger (1)
- Bakterien (1)
- Bankia sp (1)
- Basidiomycetes (1)
- Bauprodukte (1)
- Bee diseases (1)
- Bee health (1)
- Beneficial microbes (1)
- Bestandserhaltung (1)
- Bewitterung (1)
- Biodeterioration (1)
- Biodeterioration and biodegradation (1)
- Biofilm quantification (1)
- Biofilme (1)
- Bioilm (1)
- Bioinformatics virology viruses software (1)
- Biological Control (1)
- Biological control (1)
- Biologische Bekämpfung (1)
- Biomimetic (1)
- Biosynthesis (1)
- Biotische/abiotische Faktoren (1)
- Biphasic growth (1)
- Blauer Engel (1)
- C-di-GMP (1)
- COST (1)
- COST Action (1)
- COST action CA15114 AMICI (1)
- CRISPR dCas9 (1)
- Calcareous deposits (1)
- Cast iron (1)
- Cathodic protection (1)
- Cell appendages (1)
- Cell studies (1)
- Cell-envelope (1)
- Cellulose synthase (1)
- Cleridae (1)
- Coleoptera (1)
- Conditioning layer (1)
- Coniophora puteana (1)
- Conjugate (1)
- Construction Products (1)
- Contamination (1)
- Controlled atmosphere (1)
- Coptotermes (1)
- Core microbiome (1)
- Corrosion environment (1)
- Corrosion product (1)
- Cribellate spiders (1)
- Cross-feeding (1)
- Cryo XPS (1)
- CuNPs (1)
- Cultural Heritage (1)
- Cupriavidus campinensis (1)
- DIN EN 350 (1)
- DNA barcode (1)
- DSM 5009 (1)
- Dauerhaftigkeit (1)
- Decomposition point (1)
- Degradable polymer (1)
- Denitrification (1)
- Desert ecology (1)
- Deterioration (1)
- Dielectric heating (1)
- Dietary adaptation (1)
- Diffractive gratings (1)
- Direct laser writing (1)
- Disinfection Fuel (1)
- Disposal (1)
- Diversity (1)
- Drywood termite (1)
- Durability (1)
- Dye (1)
- E$volution (1)
- Ecology (1)
- Efficacy (1)
- Efflux (1)
- Electronic (1)
- Entwicklung (1)
- Environment (1)
- Environmental conditions (1)
- Environmental simulations (1)
- Essential oils (1)
- Europa (1)
- Exocrine gland (1)
- Experimental evolution (1)
- External (1)
- Extremophile (1)
- F pili (1)
- Femtosecond laser processing (1)
- Fenton-like reaction (1)
- Flammschutzmittel (1)
- Flow chamber system (1)
- Flow-System (1)
- Flow-system (1)
- Fluorescence (1)
- Fluorescence spectroscopy (1)
- Fluoride (1)
- Fluoride nanoparticles (1)
- Fluorides (1)
- Fluorolytic sol-gel synthesis (1)
- Fluorolytic sol−gel (1)
- Flügel (1)
- Food fermentation (1)
- Food safety (1)
- Force distance curve (1)
- Fuel sorption (1)
- Functionalized graphene (1)
- Fungal decay (1)
- Fungi (1)
- Fungiculture (1)
- Fungus (1)
- Fusarium (1)
- Gas storage (1)
- Gebindekonservierung (1)
- Gene expression (1)
- Genome sequence (1)
- Geological formation (1)
- Geology (1)
- Gesteinskorrosion (1)
- Graphene (1)
- Graphene–bacteria interaction (1)
- Growth-rate homeostasis (1)
- Gut symbionts (1)
- Gütezeichen (1)
- H2-Abbau (1)
- Halogenation (1)
- Halophilic (1)
- Halophilic bacteria (1)
- Hausbock (1)
- Hausbockkäfer (1)
- Healthcare (1)
- High density polyethylene (1)
- High salinity (1)
- Holzschutz, (1)
- Holzschutzmittel (1)
- Hospital premises (1)
- Hydrogen (1)
- Hydrogen permeation (1)
- Hydrogen storage (1)
- Hydrophobic interaction (1)
- Hylotrupes bajulus (1)
- Hylotrupes bajulus, Anobium punctatum, natural durability, tropical wood, native wood. (1)
- Hyperarid (1)
- Hypopharynx (1)
- IPM in museums (1)
- IR spectroscopy (1)
- ISO22196 (1)
- Implants (1)
- In situ (1)
- Industrial applications (1)
- Infections (1)
- Initial attachment (1)
- Insect evolution (1)
- Insecticide (1)
- Interdisciplinarity (1)
- Interdisciplinary (1)
- Iodine (1)
- Ionic liquid (1)
- Ionizing radiation (1)
- Isothiazolinone (1)
- Issues (1)
- Kleidermotte (1)
- Kontamination von H2 (1)
- Kraftstoffabbau (1)
- Kraftstoffbehälter (1)
- Kulturelle Entomologie (1)
- Laboratory breeding (1)
- Labrum (1)
- Lake Cadagno (1)
- Laser structuring (1)
- Laser-Materialbearbeitung (1)
- Laser-induced nanostructures (1)
- Laser-induced pariodic surface structures (1)
- Laser-induced periodic surface structueres (LIPPS) (1)
- Laser-modified surface (1)
- Library pests (1)
- Lifetime (1)
- Lightning talk (1)
- Limnoria spp (1)
- Low alloy steel (1)
- MDG ICP-ToF-MS (1)
- MIC Island (1)
- MIC projekt (1)
- Marek's virus (1)
- Marine borers (1)
- Material degradation (1)
- Materials (1)
- Mechanosensitive channel (1)
- Melting point (1)
- Melting temperature (1)
- Metabolic engineering (1)
- Metabolic flux analysis (1)
- Metabolic modeling (1)
- Metabolomics (1)
- Metal organic frameworks (1)
- Metal reducing bacteria (1)
- Metallkorrosion (1)
- Metalls (1)
- Methane (1)
- Methanogene (1)
- Methanogenesis (1)
- Methode (1)
- Microbial (1)
- Microbial adhesions (1)
- Microbial community modelling (1)
- Microbial simulation, (1)
- Microbiologically Influrenced Corrosion (MIC) (1)
- Microbiologically influenced corrosion (MIC) (1)
- Microdroplet generator (1)
- Microfluidics (1)
- Micromonospora aurantiaca (1)
- Micropatterning (1)
- Microwaves (1)
- Migration (1)
- Mikrobielle Gemeinschaft (1)
- Mircobially influcenced corrosion (1)
- Modeling (1)
- Modified mycotoxins (1)
- Moisture (1)
- Moisture performance (1)
- Molecular (1)
- Molecular clock (1)
- MscS (1)
- Mud volcanoe fluids (1)
- Multi-resistant bacteria (1)
- Multiple lines of evidence (1)
- Multiport (1)
- Museum (1)
- Museum Pests (1)
- Museum insect pest (1)
- Museum pests (1)
- Museumsschädling (1)
- Museumsschädlinge (1)
- Mussel-inspired coating (1)
- Mussel-inspired materials (1)
- Mycotoxin (1)
- Mycotoxins (1)
- Nano Particles (1)
- Nano characterization (1)
- Nano metal fluorides (1)
- Nanorods (1)
- Natural durability (1)
- Natural products discovery (1)
- Near ambient pressure XPS (1)
- Near ambient x-ray photoelectron spectroscopy (1)
- Neozoon (1)
- Netzwerk (1)
- Next generation sequencing (1)
- Nitrous oxide (1)
- Nonvolatile (1)
- Norm (1)
- Nuclear waste disposal (1)
- Oberflächenfunktionalisierung (1)
- Oil and gas industry (1)
- Oil and gas reservoir (1)
- Oilfield (1)
- One-step ionic liquids synthesis (1)
- Optical and Magnetic Materials (1)
- Osmophobic effect (1)
- Osmotic shock (1)
- Osteogenesis (1)
- P. Fluorescens (1)
- PBFSM (1)
- PE-HD (1)
- Particle synthesis (1)
- Pathogen spillover (1)
- Pathogen transmission (1)
- Performance (1)
- Permeability (1)
- Persian manuscripts (1)
- Pest control (1)
- Phenotypic heterogeneity (1)
- Phenotypic variation (1)
- Photodynamic therapy (1)
- Photonics (1)
- Pilz (1)
- Plastic degradation (1)
- Polydopamine (1)
- Polyethylene terephthalate (1)
- Polyglycerol (1)
- Polyioinic liquid (1)
- Polymer foils (1)
- Polymer-based products (1)
- Polypropylen (1)
- Polystyrol (1)
- Poster presentation (1)
- Preferential binding (1)
- Preferential exclusion (1)
- Projekte (1)
- Proteome (1)
- Pseudomonas (1)
- Pseudomonas fluorescens (1)
- Pseudomonas veronii (1)
- Ptinidae (1)
- Quasispecies (1)
- RFA (1)
- RNA-Sequencing (1)
- Radio waves (1)
- Reactor conditions (1)
- Religion (1)
- Resistance model (1)
- Rhizopus (1)
- Rhizopus and Aspergillus oryzae (1)
- Rhodonia placenta (1)
- Risikobewertung (1)
- Round robin test (1)
- Ruthenium (1)
- SEM (1)
- SEM micrography (1)
- SEM wood characterization (1)
- SEM-EDS (1)
- SIMS (1)
- SRB (1)
- Schmetterlinge (1)
- Selection (1)
- Self-assembled monolayer (1)
- Sensors (1)
- Silica and polystyrene nanoparticles (1)
- Silver (1)
- Simulation (1)
- Single cell microbiology (1)
- Sizing (1)
- Social immunity (1)
- Social insects (1)
- Software (1)
- Soil contact (1)
- Soldier (1)
- Solitary bees (1)
- Solubility (1)
- Solute excretion (1)
- Stainless steel (1)
- Stakeholder (1)
- Standardisation (1)
- Standardized test and quantification procedure (1)
- Structural color (1)
- Superhydrophilic surface (1)
- Superhydrophobic surface (1)
- Suppression (1)
- Surface functionalization (1)
- Survival rates (1)
- Symbiosis (1)
- Synchrotron-XPS (1)
- Synergy (1)
- Synthetic metabolism (1)
- Temperature and humidity (1)
- Teredo sp (1)
- Termite control (1)
- Termite-associated microbes (1)
- Termiten (1)
- Termitoidae (1)
- Textilschädling (1)
- Thermodynamic (1)
- Thioimidazolium (1)
- ToF SIMS (1)
- ToF-SIMS (1)
- Touch surfaces (1)
- Trait-based ecology (1)
- Transcriptomics (1)
- Ultrakurzpuls-Laser (1)
- Ultrashort laser processing (1)
- Ultrastructure (1)
- Unterirdische Speicher (1)
- UpConversion (1)
- Velocimetry (1)
- Vererbung (1)
- Virology (1)
- Virulence (1)
- Viruses (1)
- Wasserstofflagerung (1)
- Water (1)
- Water atmosphere (1)
- Water stable isotope analysis (1)
- Water uptake (1)
- White-rot fungi (1)
- Wirksamkeit (1)
- Wood Modification (1)
- X-ray spectroscopic techniques (1)
- X-ray tomographic (1)
- XANES (1)
- XRF (1)
- Xestobium rufovillosum (1)
- Zearalenone (1)
- Zearalenone sulfate (1)
- a-zearalenol (1)
- biocide tolerance (1)
- disinfection (1)
- dsDAN monolayer (1)
- evolution (1)
- fs-laser processing (1)
- heterogeneity (1)
- iChip (1)
- pH probe (1)
- pH sensor (1)
- underexplored phyla (1)
Organisationseinheit der BAM
- 4.1 Biologische Materialschädigung und Referenzorganismen (266) (entfernen)
Paper des Monats
- ja (6)
Eingeladener Vortrag
- nein (91)
AbstractThis work addresses the critical need for multifunctional materials and substrate‐independent high‐precision surface modification techniques that are essential for advancing microdevices and sensing elements. To overcome existing limitations, the versatility of mussel‐inspired materials (MIMs) is combined with state‐of‐the‐art multiphoton direct laser writing (DLW) microfabrication. In this way, 2D and 3D MIM microstructures of complex designs are demonstrated with sub‐micron to micron resolution and extensive post‐functionalization capabilities. This study includes polydopamine (PDA), mussel‐inspired linear, and dendritic polyglycerols (MI‐lPG and MI‐dPG), allowing their direct microstructure on the substrate of choice with the option to tailor the patterned topography and morphology in a controllable manner. The functionality potential of MIMs is demonstrated by successfully immobilizing and detecting single‐stranded DNA on MIM micropattern and nanoarray surfaces. In addition, easy modification of MIM microstructure with silver nanoparticles without the need of any reducing agent is shown. The methodology developed here enables the integration of MIMs in advanced applications where precise surface functionalization is essential.
Bacterial biofilms pose serious problems in medical and industrial settings. One of the major societal challenges lies in the increasing resistance of bacteria against biocides used in antimicrobial treatments, e.g., via overabundant use in medicine, industry, and agriculture or cleaning and disinfection in private households. Hence, new efficient bacteria-repellent strategies avoiding the use of biocides are strongly desired. One promising route to achieve bacteria-repellent surfaces lies in the contactless and aseptic large-area laser-processing of technical surfaces. Tailored surface textures, enabled by different laser-processing strategies that result in topographic scales ranging from nanometers to micrometers may provide a solution to this challenge. This article presents a current state-of-the-art review of laser-surface subtractive texturing approaches for controlling the biofilm formation for different bacterial strains and in different environments. Based on specific properties of bacteria and laser-processed surfaces, the challenges of anti-microbial surface designs are discussed, and future directions will be outlined.
Bacterial biofilms can pose a serious health risk to humans and are less susceptible
to antibiotics and disinfection than planktonic bacteria. Here, a novel method
for biofilm eradication based on antimicrobial photodynamic therapy utilizing
a nanoparticle in conjunction with a BODIPY derivative as photosensitizer was
developed. Reactive oxygen species are generated upon illumination with
visible light and lead to a strong, controllable and persistent eradication of
both planktonic bacteria and biofilms. One of the biggest challenges in biofilm
eradication is the penetration of the antimicrobial agent into the biofilm and
its matrix. A biocompatible hydrophilic nanoparticle was utilized as a delivery
system for the hydrophobic BODIPY dye and enabled its accumulation within the
biofilm. This key feature of delivering the antimicrobial agent to the site of action
where it is activated resulted in effective eradication of all tested biofilms. Here,
3 bacterial species that commonly form clinically relevant pathogenic biofilms
were selected: Escherichia coli, Staphylococcus aureus and Streptococcus
mutans. The development of this antimicrobial photodynamic therapy tool for
biofilm eradication takes a promising step towards new methods for the much
needed treatment of pathogenic biofilms.
Bei dem Vortrag werden die potentiellen mikrobiologischen Einflüsse im Falle der Lagerung von Wasserstofflagerung in unterirdischen geologischen Formationen vorgestellt, die Zusammenarbeit mit internen und auch externen Partnern. Der Fokus liegt dabei auf UGF; anaerobe Umgebungen, Sulfate reduzierenden Bakterien, methanogenen Archaea, weiterleitenden Systeme und die Kombination der Untersuchung abiotisch/biotische
Biocides are used for a wide range of purposes, including disinfectants or preservatives. They play a major role in the prevention of microbial infections in healthcare and animal husbandry. The use of biocides often leads to the discharge of active biocidal substances into wastewater streams, causing the exposure of wastewater microbial communities to subinhibitory concentrations. In turn, it is known that wastewater treatment plants (WWTP) are hotspots for antibiotic resistant bacteria. Since similar mechanisms confer resistance to biocides and antibiotics, exposure to biocides can result in co-selection of antibiotic resistant bacteria in WWTP Here, we want to investigate co-selection processes of antibiotic resistance in natural WWTP microbial communities upon biocide exposure. Microbial communities were sampled at the WWTP Ruhleben in Berlin and characterized regarding their susceptibility against different clinically relevant antibiotics. To investigate the link between biocide exposure and antibiotic resistance, changes in the susceptibility level after exposure to environmentally relevant concentrations of the commonly used biocide didecyldimethylammonium chloride (DDAC) will be determined by enumerating resistant and non-resistant E. coli on selective plates with and without antibiotics and DDAC. In case of antibiotics, clinical breakpoint concentrations according to EUCAST will be used to discriminate between susceptible and resistant strains. In case of DDAC (and biocides in general), clinical breakpoints do not exist. Therefore, we determined a cut-off concentration at which the majority of naturally-occurring E. coli strains cannot grow anymore based on (I) the MIC (minimal inhibitory concentration) distribution, and (II) by plating wastewater communities onto selective indicator agar plates loaded with increasing DDAC concentration. Additionally, antibiotic cross-resistance will be determined by spotting single colonies, isolated from DDAC-selective plates onto antibiotic plates. The results of our experiments will help to determine selective concentrations and to estimate the risk of antibiotic co-selection and cross-resistance in microbial WWTP communities upon biocide exposure.
Background. Disinfection is an important mitigation strategy to control and prevent the spread of infections. Incomplete or incorrect usage of disinfection may promote evolution of resistance against disinfectants and antibiotics. Ideally, disinfection reduces the number of surviving bacteria and the chance for resistance evolution. Resistance describes the ability to grow in previously inhibitory concentrations of an antimicrobial, whereas tolerance is associated with enhanced survival of lethal doses. Individual bacteria from the same population can display considerable heterogeneity in their ability to survive treatment (i.e. tolerance) with antimicrobials, which can result in unexpected treatment failure.
Objective. In this study, we investigated six active substances of disinfectants, preservatives, and antiseptics against a population of E. coli to identify the presence of a tolerant subpopulation.
Methods. We performed time-kill experiments and analyzed the data with a mathematical model to statistically infer whether the data is best explained by the presence of a tolerant subpopulation.
Results. The analysis identified bimodal kill kinetics for benzalkonium chloride, didecyldimethylammonium chloride, and isopropanol. In contrast, kill kinetics by chlorhexidine, glutaraldehyde, and hydrogen peroxide were best explained by unimodal kill kinetics. These findings have implications for the risk of disinfection failure. In addition, we are currently performing adaptive laboratory evolution (ALE) experiments with the different disinfectants to investigate the potential consequences of tolerant sub-populations for the evolution of antimicrobial resistance and tolerance.
Background. Disinfection is an important mitigation strategy to control and prevent the spread of infections. Incomplete or incorrect usage of disinfection may promote evolution of resistance against disinfectants and antibiotics. Ideally, disinfection reduces the number of surviving bacteria and the chance for resistance evolution. Resistance describes the ability to grow in previously inhibitory concentrations of an antimicrobial, whereas tolerance is associated with enhanced survival of lethal doses. Individual bacteria from the same population can display considerable heterogeneity in their ability to survive treatment (i.e. tolerance) with antimicrobials, which can result in unexpected treatment failure.
Objective. In this study, we investigated six active substances of disinfectants, preservatives, and antiseptics against a population of E. coli to identify the presence of a tolerant subpopulation.
Methods. We performed time-kill experiments and analyzed the data with a mathematical model to statistically infer whether the data is best explained by the presence of a tolerant subpopulation.
Results. The analysis identified bimodal kill kinetics for benzalkonium chloride, didecyldimethylammonium chloride, and isopropanol. In contrast, kill kinetics by chlorhexidine, glutaraldehyde, and hydrogen peroxide were best explained by unimodal kill kinetics. These findings have implications for the risk of disinfection failure. In addition, we are currently performing adaptive laboratory evolution (ALE) experiments with the different disinfectants to investigate the potential consequences of tolerant sub-populations for the evolution of antimicrobial resistance and tolerance.
Surfaces of metallic copper and copper alloys effectively inactivate microorganisms and viruses. However, the exact inactivation mode is still under debate. Main factors are assumed to include direct contact with the metallic surface, influx of Cu(I)/Cu(II) ions and the generation of reactive oxygen species (ROS). Laser-induced periodic surface structures (LIPSS) are frequently reported to act antibacterial, mainly by prevention of bacterial adhesion due to a limited number of possible adhesion points or by increasing the overall surface of intrinsically antibacterial materials. In time-kill experiments with E. coli and S. aureus we analyzed the impact of LIPSS on the toxicity of metallic copper and brass. We also conducted ROS accumulation assays and conclude that the application of LIPSS is not generally straight forward to obtain or improve antibacterial surfaces. Thus, the antibacterial effects of LIPPS.
Bacteria are ubiquitous and colonize all types of surfaces, including those in close proximity to humans, such as skin, food, and everyday objects. This raises the question of whether their presence represents a problem to be mitigated or a potential source of benefit to be harnessed, thereby stimulating scientific inquiry into the role of surface-associated bacteria in diverse domains ranging fromhuman health to industrial biotechnology. Aim: The objective of this project is to explore the impact of modifying surface topography on bacterial adhesion behavior. By manipulating the physical characteristics of the substrate, the attachment and detachment dynamics of bacteria can potentially be modified, leading to novel strategies for controlling bacterial colonization in various applications, such as medical devices. Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) were tested on LIPSS-covered Fused Silica samples.