4 Material und Umwelt
Filtern
Dokumenttyp
- Posterpräsentation (137) (entfernen)
Sprache
- Englisch (137) (entfernen)
Referierte Publikation
- nein (137)
Schlagworte
- Biocides (19)
- Corrosion (10)
- Antimicrobial resistance (9)
- Biofilm (8)
- Methanogens (7)
- Persistence (7)
- Biocide (6)
- Horizontal gene transfer HGT (6)
- Microbiology (6)
- Resistance (6)
- Resistance evolution (6)
- Archaea (5)
- Bacteria (5)
- MIC (5)
- Biofilms (4)
- Evolution (4)
- Nano (4)
- Per- and Polyfluoroalkyl substances (PFAS) (4)
- Raman spectroscopy (4)
- VOC (4)
- Bacterial adhesion (3)
- Biocide tolerance (3)
- DHN melanin (3)
- Disinfection (3)
- E. coli (3)
- Environmental Simulation (3)
- Fertilzer (3)
- Fungus (3)
- Genetics (3)
- Ground water (3)
- Ink (3)
- Knufia petricola (3)
- LIPSS (3)
- Microbiologically influenced corrosion (3)
- OECD (3)
- PFAS (3)
- Reference material (3)
- Single cell analysis (3)
- Wastewater (3)
- XRF (3)
- ATR (2)
- Aluminium (2)
- Analytical method (2)
- Antibiotic resisitance (2)
- Antibiotics (2)
- Antimicrobials (2)
- Biofilm formation (2)
- Capsules (2)
- Cascade impactor (2)
- Chamber test (2)
- Combustion ion chromatography (2)
- Construction products (2)
- Corrosion products (2)
- Cross-resistance (2)
- Desinfectant (2)
- E.coli (2)
- EN 16516 (2)
- Emission (2)
- FIB/SEM (2)
- Glutaraldehyde (2)
- Halomonas CRISPR Ectoine (2)
- Heterogeneity (2)
- Horizontal gene transfer (2)
- ISO 16000-6 (2)
- Inks (2)
- Iron (2)
- Laser-induced periodic surface structures (LIPSS) (2)
- Leaching (2)
- Manuscript (2)
- Material emissions (2)
- Melanin (2)
- Mutation rate (2)
- NAP-XPS (2)
- Non-invasive analysis (2)
- Optics (2)
- Persister cells (2)
- Phosphorus (2)
- Phosphorus recovery (2)
- Prüfrichtlinie (2)
- Quality assurance (2)
- Raman Spectroscopy (2)
- Reference materials (2)
- Reflectography (2)
- Reverse glass painting (2)
- Risk assesment (2)
- Rock-inhabiting fungus (2)
- Round robin test (2)
- SC-ICP-ToF-MS (2)
- Sc-ICP-ToF-MS (2)
- Tolerance (2)
- VVOCs (2)
- Volatile organic compounds (2)
- XANES spectroscopy (2)
- Adsorbable organically bound fluorine (AOF) (1)
- Aerosol (1)
- Aerosol element alanysis (1)
- Aerosol element mass concentration (1)
- Affinity chromatography (1)
- Affinity extraction (1)
- Affinity support (1)
- Agronomic performance (1)
- Air exchange rate (1)
- Alginate (1)
- Alite (1)
- Aluminum (1)
- Aluminum oxide (1)
- Amino acid analysis (1)
- Analytical standards (1)
- Anodization (1)
- Antagonism (1)
- Anti-microbial effects (1)
- Antibodies (1)
- Antibody purification (1)
- Antimicrobial (1)
- Antimicrobial surfaces (1)
- Antimony (1)
- Aromatic amino acid analysis AAAA (1)
- Artificial ageing (1)
- Astrobiology (1)
- BFR (1)
- BSA (1)
- Bacterial growth (1)
- Bio-receptive (1)
- Bioactive glass (1)
- Bioavailability (1)
- Biocorrosion (1)
- Biodegradation (1)
- Biodiversity (1)
- Biofilm growth (1)
- Biosynthesis (1)
- Biotechnology (1)
- Black fungus (1)
- Bottom ash (1)
- Bovine serum albumin (1)
- Building product (1)
- Carbon steel corrosion (1)
- Chamber-test (1)
- Chicken manure (1)
- Chromium (1)
- Circular economy (1)
- Co-combustion (1)
- Combustion Ion Chromatography (1)
- Combustion ion chromatography (CIC) (1)
- Comsumer good samples (1)
- Concrete (1)
- Conservation (1)
- Consumer articles (1)
- Coptic (1)
- Crispr-Cas9 (1)
- Cryptoendolithic black fungus (1)
- Crystallization (1)
- DGT (1)
- DRIFTS (1)
- DSLT (1)
- Damage repair (1)
- Diffusive Gradients in thin films (DGT) (1)
- Diffusive gradients in thin films (DGT) (1)
- Diffusive sampling (1)
- Downstream processing (1)
- EDX Analysis (1)
- ESEM (1)
- Emission chamber testing (1)
- Emissions (1)
- European standard (1)
- Experimental evolution (1)
- Extracellular polymeric substances (1)
- Extractable organically bound fluorine (EOF) (1)
- Extremotolerance (1)
- Façade (1)
- Femtosecond laser processing (1)
- Fertilizer (1)
- Fiber (1)
- Fibre (1)
- Flow Model (1)
- Flow chamber system (1)
- Flow-System (1)
- Fluorescence (1)
- Fluorescent proteins (1)
- Food safety (1)
- Foward genetics (1)
- Fractional factorial (1)
- Gas chromatography - mass spectrometry (GC-MS) (1)
- Genetic engineering (1)
- Genome sequence (1)
- Geosynthetics (1)
- Glass paints (1)
- Guideline (1)
- Gypsum recycling (1)
- HAXPES (1)
- Headspace GC-MS (1)
- Herculaneum (1)
- Herteogeneous phenotypes (1)
- Heterologous expression (1)
- Hi-Tension (1)
- High resolution-continuum source-graphite furnace molecular absorption spectrometry (HR-CS-GFMAS) (1)
- Historic buildings (1)
- Human plasma (1)
- IAQ (1)
- ISO22196 (1)
- Implants (1)
- In-vivo mutagenesis (1)
- Iodine (1)
- Ir DNA staining approach (1)
- Iron oxide nanoparticles (1)
- Korrosion (1)
- LCA (1)
- LIBS (1)
- Laser structuring (1)
- Laser-induced pariodic surface structures (1)
- Loading factor (1)
- Lysimeter (1)
- MDG ICP-ToF-MS (1)
- MIC projekt (1)
- Manuscripts (1)
- Material degradation (1)
- Mathematical modelling (1)
- Medieval glasses (1)
- Metal organic frameworks (1)
- Method development (1)
- Micro plastic (1)
- Microbiological influenced corrosion MIC (1)
- Microbiologically Influrenced Corrosion (MIC) (1)
- Microcolonial fungi (1)
- Microdroplet generator (1)
- Microfluidics (1)
- Mikrobiell beeinflusste Korrosion (1)
- Mikroplastik (1)
- Mobile anodisation (1)
- Mobile application (1)
- Modelling (1)
- Modern painting (1)
- Mycotoxins (1)
- Nano particle (1)
- NanoSIMS (1)
- Nanomaterial (1)
- Nanomaterials (1)
- Nanoparticles (1)
- Nanopartikel (1)
- Nitrification inhibitor (1)
- Nitrogen (1)
- Non invasive analysis (1)
- Odour (1)
- Organo fluorine analysis (1)
- PAK (1)
- PP (1)
- PS (1)
- Papyrus (1)
- Parchment (1)
- Particle size (1)
- Particle size distributuion (1)
- Passive sampling (1)
- Per- and polyfluorinated alkyl substances (PFASs) (1)
- Perceived Intensity (1)
- Perfluorocarboxylic acid (PFCA) (1)
- Phosphonic acids (1)
- Phosphorus recycling (1)
- Pigment (1)
- Plant growth test (1)
- Plant-availability (1)
- Pollutant (1)
- Polyfluorinated alcohol (PFOH) (1)
- Polyglycerol (1)
- Polymer (1)
- Portland Cement (1)
- Poster presentation (1)
- Pot experiment (1)
- Proficiency Test (1)
- Protein a (1)
- Protein hydrolysis (1)
- Protein immobilization (1)
- Protein quantification (1)
- Pseudomonas (1)
- QA/QC (1)
- Reactive oxygen species (1)
- Red Mud (1)
- Reductive amination (1)
- Reductive iron assimilation (1)
- Remediation (1)
- Reverse painting on glass (1)
- Rock-inhabiting fungi (1)
- SCALE (1)
- SDS-PAGE (1)
- SPE extraction (1)
- Sapphire (1)
- Scandium (1)
- Schwermetalle (1)
- Selection (1)
- Sewage extraction (1)
- Sewage sludge (1)
- Sewage sludge ash (1)
- Siderophore (1)
- Signal transduction (1)
- Single cell (1)
- Sintering (1)
- Size distribution (1)
- Small angle x-ray scattering (1)
- Soil (1)
- Soil P species (1)
- Soil extraction (1)
- Solubility (1)
- Sorption (1)
- Spectroscopy (1)
- Stained glass windows (1)
- Stained glasses (1)
- Standardized test and quantification procedure (1)
- Steelmaking slag (1)
- Structural color (1)
- Sugarcane bagasse (1)
- Sum parameter analyis (1)
- Superhydrophilic surface (1)
- Superhydrophobic surface (1)
- Surfacewaters (1)
- Synchrotron (1)
- Synchrotron-XPS (1)
- Synthetic organic pigments (1)
- TED-GC/MS (1)
- TEM (1)
- TXRF (1)
- TXRF ambient air aerosol chemical analysis aerosol element composition (1)
- Termites (1)
- Test Guideline (1)
- Testguideline (1)
- Thermal extraction (1)
- Transmission electron microscopy (1)
- Tricalcium-silicate (1)
- Tyrosine (1)
- UFP (1)
- Ultrashort PFAS (1)
- Ultrashort laser processing (1)
- Uptake rate (1)
- VOC-Emission (1)
- VOC-emission (1)
- Velocimetry (1)
- Volatile organic compound (1)
- Weathering (1)
- X-ray adsorption near-edge structure (XANES) spectroscopy (1)
- X-rays (1)
- XPS (1)
- XRF Analysis (1)
- Zeolite (1)
- biocide tolerance (1)
- black fungi (1)
- disinfection (1)
- evolution (1)
- fs-laser processing (1)
- genetics (1)
- genomics (1)
- heterogeneity (1)
Organisationseinheit der BAM
- 4 Material und Umwelt (137) (entfernen)
This study details a thorough analysis of leaky and waveguide modes in biperiodic diffractive nanostructures. By tuning diffraction orders and subsequently confining local density of optical states at two distinct resonance wavelengths, we present a highly sensitive refractive index biosensing platform that can resolve 35.5 to 41.3 nm/RIU of spectral shift for two separate biological analytes.
Algal biofilm façades are an alternative to traditional green façades which can help to improve biodiversity and air quality within cities. They present a low maintenance approach in which subaerial algae are grown directly on concrete substrates. The intrinsic bioreceptivity of the substrate is a critical factor in successful facade colonisation. Existing research has identified several environmental and material properties which influence concrete bioreceptivity, however a consensus has yet to be made on which properties are most influential and how the interaction between properties may promote algal biofilm growth under specific conditions.
The amount of plant-available phosphorus (P) in soil strongly influences the yield of plants in agriculture. Therefore, various simple chemical extraction methods have been developed to estimate the plant-available P pools in soil. More recently, several experiments with the DGT technique have shown that it has a much better correlation to plant-available P in soils than standard chemical extraction methods (e.g. calcium-acetate-lactate (CAL), Colwell, Olsen, water) when soils with different characteristics are considered. However, the DGT technique cannot give information on the plant-available P species in the soil. Therefore, we combined DGT with solution 31P nuclear magnetic resonance (NMR) spectroscopy. This was achieved by using a modified DGT device in which the diffusive layer had a larger pore size, the binding layer incorporated an adsorption material with a higher capacity, and the device had a larger exposure area. The spectroscopic investigation was undertaken after elution of the deployed DGT binding layer in a NaOH solution. Adsorption tests using solutions of known organic P compounds showed that a sufficient amount of these compounds could be adsorbed on the binding layer in order for them to be analyzed by solution 31P NMR spectroscopy. Furthermore, various intermediates of the hydrolysis of trimetaphosphate in soil could be also analyzed over time.
Biocides are used for a wide range of purposes, including disinfectants or preservatives. They play a major role in the prevention of microbial infections in healthcare and animal husbandry. The use of biocides often leads to the discharge of active biocidal substances into wastewater streams, causing the exposure of wastewater microbial communities to subinhibitory concentrations. In turn, it is known that wastewater treatment plants (WWTP) are hotspots for antibiotic resistant bacteria. Since similar mechanisms confer resistance to biocides and antibiotics, exposure to biocides can result in co-selection of antibiotic resistant bacteria in WWTP Here, we want to investigate co-selection processes of antibiotic resistance in natural WWTP microbial communities upon biocide exposure. Microbial communities were sampled at the WWTP Ruhleben in Berlin and characterized regarding their susceptibility against different clinically relevant antibiotics. To investigate the link between biocide exposure and antibiotic resistance, changes in the susceptibility level after exposure to environmentally relevant concentrations of the commonly used biocide didecyldimethylammonium chloride (DDAC) will be determined by enumerating resistant and non-resistant E. coli on selective plates with and without antibiotics and DDAC. In case of antibiotics, clinical breakpoint concentrations according to EUCAST will be used to discriminate between susceptible and resistant strains. In case of DDAC (and biocides in general), clinical breakpoints do not exist. Therefore, we determined a cut-off concentration at which the majority of naturally-occurring E. coli strains cannot grow anymore based on (I) the MIC (minimal inhibitory concentration) distribution, and (II) by plating wastewater communities onto selective indicator agar plates loaded with increasing DDAC concentration. Additionally, antibiotic cross-resistance will be determined by spotting single colonies, isolated from DDAC-selective plates onto antibiotic plates. The results of our experiments will help to determine selective concentrations and to estimate the risk of antibiotic co-selection and cross-resistance in microbial WWTP communities upon biocide exposure.
Background. Disinfection is an important mitigation strategy to control and prevent the spread of infections. Incomplete or incorrect usage of disinfection may promote evolution of resistance against disinfectants and antibiotics. Ideally, disinfection reduces the number of surviving bacteria and the chance for resistance evolution. Resistance describes the ability to grow in previously inhibitory concentrations of an antimicrobial, whereas tolerance is associated with enhanced survival of lethal doses. Individual bacteria from the same population can display considerable heterogeneity in their ability to survive treatment (i.e. tolerance) with antimicrobials, which can result in unexpected treatment failure.
Objective. In this study, we investigated six active substances of disinfectants, preservatives, and antiseptics against a population of E. coli to identify the presence of a tolerant subpopulation.
Methods. We performed time-kill experiments and analyzed the data with a mathematical model to statistically infer whether the data is best explained by the presence of a tolerant subpopulation.
Results. The analysis identified bimodal kill kinetics for benzalkonium chloride, didecyldimethylammonium chloride, and isopropanol. In contrast, kill kinetics by chlorhexidine, glutaraldehyde, and hydrogen peroxide were best explained by unimodal kill kinetics. These findings have implications for the risk of disinfection failure. In addition, we are currently performing adaptive laboratory evolution (ALE) experiments with the different disinfectants to investigate the potential consequences of tolerant sub-populations for the evolution of antimicrobial resistance and tolerance.
Background. Disinfection is an important mitigation strategy to control and prevent the spread of infections. Incomplete or incorrect usage of disinfection may promote evolution of resistance against disinfectants and antibiotics. Ideally, disinfection reduces the number of surviving bacteria and the chance for resistance evolution. Resistance describes the ability to grow in previously inhibitory concentrations of an antimicrobial, whereas tolerance is associated with enhanced survival of lethal doses. Individual bacteria from the same population can display considerable heterogeneity in their ability to survive treatment (i.e. tolerance) with antimicrobials, which can result in unexpected treatment failure.
Objective. In this study, we investigated six active substances of disinfectants, preservatives, and antiseptics against a population of E. coli to identify the presence of a tolerant subpopulation.
Methods. We performed time-kill experiments and analyzed the data with a mathematical model to statistically infer whether the data is best explained by the presence of a tolerant subpopulation.
Results. The analysis identified bimodal kill kinetics for benzalkonium chloride, didecyldimethylammonium chloride, and isopropanol. In contrast, kill kinetics by chlorhexidine, glutaraldehyde, and hydrogen peroxide were best explained by unimodal kill kinetics. These findings have implications for the risk of disinfection failure. In addition, we are currently performing adaptive laboratory evolution (ALE) experiments with the different disinfectants to investigate the potential consequences of tolerant sub-populations for the evolution of antimicrobial resistance and tolerance.
Surfaces of metallic copper and copper alloys effectively inactivate microorganisms and viruses. However, the exact inactivation mode is still under debate. Main factors are assumed to include direct contact with the metallic surface, influx of Cu(I)/Cu(II) ions and the generation of reactive oxygen species (ROS). Laser-induced periodic surface structures (LIPSS) are frequently reported to act antibacterial, mainly by prevention of bacterial adhesion due to a limited number of possible adhesion points or by increasing the overall surface of intrinsically antibacterial materials. In time-kill experiments with E. coli and S. aureus we analyzed the impact of LIPSS on the toxicity of metallic copper and brass. We also conducted ROS accumulation assays and conclude that the application of LIPSS is not generally straight forward to obtain or improve antibacterial surfaces. Thus, the antibacterial effects of LIPPS.
Bacteria are ubiquitous and colonize all types of surfaces, including those in close proximity to humans, such as skin, food, and everyday objects. This raises the question of whether their presence represents a problem to be mitigated or a potential source of benefit to be harnessed, thereby stimulating scientific inquiry into the role of surface-associated bacteria in diverse domains ranging fromhuman health to industrial biotechnology. Aim: The objective of this project is to explore the impact of modifying surface topography on bacterial adhesion behavior. By manipulating the physical characteristics of the substrate, the attachment and detachment dynamics of bacteria can potentially be modified, leading to novel strategies for controlling bacterial colonization in various applications, such as medical devices. Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) were tested on LIPSS-covered Fused Silica samples.
Fungal biofilms on materials: describing and modelling growth of the black fungus Knufia petricola
(2023)
Fungi that grow as biofilms are associated with clinical settings as well as various cases of material fouling and material damage. Black fungi as biofilm formers have been rarely studied so far. Their conspicuous dark pigmentation, EPS production, adhesion capabilities and adaptations to stresses allow black fungi to develop biofilms on materials under harsh conditions. For example, rock-inhabiting black fungi withstand sun irradiation and dehydration and are therefore ubiquitous on arid surfaces like solar panels and marble monuments.
To understand and control their ability to colonise and deteriorate materials, one should assess and model black fungi’s growth patterns. But so far, no mathematical model has been developed to describe their growth. Knufia petricola A95, representing rock-inhabiting fungi from Chaetothyriales, is genetically amenable and can serve as a model for biofilm studies in black fungi. The primary objective of this project is to develop a growth model for K. petricola A95 which will enable to define and predict material colonisation of black fungi.
Dedicated experimental work with K. petricola will allow the quantitative assessment of the impact of environmental conditions (e.g. pH, nutrients, etc.) on the growth behaviour at the biofilm and single cells level. Data which will be used to validate and develop an individual-based model (based on the iDynoMICS modelling platform) that explains how fungal biofilms form, colonise materials, and cause deterioration.
Thus far, research has been conducted on the impact of different concentrations and sources of major elements (e.g. C, N, …), as well as trace elements (e.g. Cu, Mg, …), on the colony shape and biomass of Knufia petricola A95 biofilms. To study the behaviour of single cells, the length of the cell cycle in different growth media has been determined via the combined use of microfluidic devices and confocal microscopy.
Dihydroxynaphthalene (DHN) melanin is produced by diverse Ascomycetes via slightly differing biosynthetic routes. The polyketide synthases (PKS) release the heptaketide YWA1, the hexaketide AT4HN or the pentaketide T4HN. The first two products are deacetylated by ‘yellowish-green’ hydrolases to T4HN, and T4HN is further converted by a core set of enzymes to DHN. Final polymerization steps are accomplished by multicopper oxidases. DHN melanogenesis is often regulated in a spatial and temporal fashion resulting e.g., in melanized reproduction and survival structures of the foliar plant pathogen Botrytis cinerea (Schumacher 2016, Mol Microbiol). In contrast, microcolonial black fungi exhibit constitutive melanogenesis. Here, DHN melanin builds a protective layer around all vegetative cells thus contributing to the survival of diverse environmental stresses even without specialized reproduction structures. For studying the regulation and relevance of DHN melanogenesis for tolerance of abiotic and biotic stresses, adhesion to substrates and subsequent damage of colonized surfaces, the rock-inhabiting fungus Knufia petricola was chosen as gene functions in this fungus can be studied by CRISPR/Cas9-based genome editing. The putative melanogenic genes were identified in the genome of K. petricola, deleted to confirm their involvement in DHN melanogenesis and co-expressed in Saccharomyces cerevisiae for reconstruction of the synthesis pathway. Phenotypes of deletion mutants are studied for specifying the functions of DHN melanin in K. petricola.