4 Material und Umwelt
Filtern
Dokumenttyp
- Posterpräsentation (146) (entfernen)
Referierte Publikation
- nein (146)
Schlagworte
- Biocides (19)
- Corrosion (10)
- Antimicrobial resistance (9)
- Biofilm (8)
- Methanogens (7)
- Persistence (7)
- Biocide (6)
- Horizontal gene transfer HGT (6)
- Microbiology (6)
- Resistance (6)
- Resistance evolution (6)
- Archaea (5)
- Bacteria (5)
- MIC (5)
- Biofilms (4)
- Evolution (4)
- Fungus (4)
- Nano (4)
- Per- and Polyfluoroalkyl substances (PFAS) (4)
- Raman spectroscopy (4)
- VOC (4)
- XRF (4)
- Aluminium (3)
- Bacterial adhesion (3)
- Biocide tolerance (3)
- DHN melanin (3)
- Disinfection (3)
- E. coli (3)
- Environmental Simulation (3)
- Fertilzer (3)
- Genetics (3)
- Ground water (3)
- Ink (3)
- Knufia petricola (3)
- LIPSS (3)
- Leaching (3)
- Microbiologically influenced corrosion (3)
- OECD (3)
- PFAS (3)
- Reference material (3)
- Single cell analysis (3)
- Wastewater (3)
- ATR (2)
- Analytical method (2)
- Antibiotic resisitance (2)
- Antibiotics (2)
- Antimicrobials (2)
- Biofilm formation (2)
- Capsules (2)
- Cascade impactor (2)
- Chamber test (2)
- Combustion ion chromatography (2)
- Construction products (2)
- Corrosion products (2)
- Cross-resistance (2)
- Desinfectant (2)
- E.coli (2)
- EN 16516 (2)
- ESEM (2)
- Emission (2)
- FIB/SEM (2)
- Genetic engineering (2)
- Glutaraldehyde (2)
- Halomonas CRISPR Ectoine (2)
- Heterogeneity (2)
- Horizontal gene transfer (2)
- ISO 16000-6 (2)
- Inks (2)
- Iron (2)
- Laser-induced periodic surface structures (LIPSS) (2)
- Manuscript (2)
- Material emissions (2)
- Melanin (2)
- Mutation rate (2)
- NAP-XPS (2)
- Non-invasive analysis (2)
- Optics (2)
- Persister cells (2)
- Phosphorus (2)
- Phosphorus recovery (2)
- Prüfrichtlinie (2)
- Quality assurance (2)
- Raman Spectroscopy (2)
- Reference materials (2)
- Reflectography (2)
- Reverse glass painting (2)
- Risk assesment (2)
- Rock-inhabiting fungus (2)
- Round robin test (2)
- SC-ICP-ToF-MS (2)
- SCALE (2)
- Sc-ICP-ToF-MS (2)
- Scandium (2)
- Sorption (2)
- Tolerance (2)
- VVOCs (2)
- Volatile organic compounds (2)
- XANES spectroscopy (2)
- XPS (2)
- Additive Fertigung (1)
- Adsorbable organically bound fluorine (AOF) (1)
- Aerosol (1)
- Aerosol element alanysis (1)
- Aerosol element mass concentration (1)
- Affinity chromatography (1)
- Affinity extraction (1)
- Affinity support (1)
- Agronomic performance (1)
- Air exchange rate (1)
- Air pollution (1)
- Alginate (1)
- Alite (1)
- Aluminum (1)
- Aluminum oxide (1)
- Amino acid analysis (1)
- Analytical standards (1)
- Analytik (1)
- Anodization (1)
- Antagonism (1)
- Anti-microbial effects (1)
- Antibodies (1)
- Antibody purification (1)
- Antimicrobial (1)
- Antimicrobial surfaces (1)
- Antimony (1)
- Aromatic amino acid analysis AAAA (1)
- Artificial ageing (1)
- Astrobiology (1)
- BFR (1)
- BSA (1)
- Bacterial growth (1)
- Bio-receptive (1)
- Bioactive glass (1)
- Bioavailability (1)
- Biocorrosion (1)
- Biodegradation (1)
- Biodiversity (1)
- Biofilm growth (1)
- Biosynthesis (1)
- Biotechnology (1)
- Black fungus (1)
- Bottom ash (1)
- Bovine serum albumin (1)
- Building product (1)
- Carbon steel corrosion (1)
- Cell factory (1)
- Chamber-test (1)
- Chicken manure (1)
- Chromium (1)
- Circular economy (1)
- Co-combustion (1)
- Combustion Ion Chromatography (1)
- Combustion ion chromatography (CIC) (1)
- Comsumer good samples (1)
- Concrete (1)
- Conservation (1)
- Consumer articles (1)
- Coptic (1)
- Crispr-Cas9 (1)
- Cryptoendolithic black fungus (1)
- Crystallization (1)
- DGT (1)
- DRIFTS (1)
- DSLT (1)
- Damage repair (1)
- Diffusive Gradients in thin films (DGT) (1)
- Diffusive gradients in thin films (DGT) (1)
- Diffusive sampling (1)
- Downstream processing (1)
- EDX Analysis (1)
- Emission chamber testing (1)
- Emission testing (1)
- Emissions (1)
- Ersatzbaustoffe (1)
- European standard (1)
- Experimental evolution (1)
- Extracellular polymeric substances (1)
- Extractable organically bound fluorine (EOF) (1)
- Extremotolerance (1)
- FFF-3D printing (1)
- Façade (1)
- Femtosecond laser processing (1)
- Fertilizer (1)
- Fiber (1)
- Fibre (1)
- Flame-retardant (1)
- Flow Model (1)
- Flow chamber system (1)
- Flow-System (1)
- Fluorescence (1)
- Fluorescent proteins (1)
- Food safety (1)
- Foward genetics (1)
- Fractional factorial (1)
- Gas chromatography - mass spectrometry (GC-MS) (1)
- Genome sequence (1)
- Geosynthetics (1)
- Glasanalyse (1)
- Glasmalfarben (1)
- Glass paints (1)
- Guideline (1)
- Gypsum recycling (1)
- HAXPES (1)
- Headspace GC-MS (1)
- Herculaneum (1)
- Herteogeneous phenotypes (1)
- Heterologous expression (1)
- Heterologous gene expression (1)
- Hi-Tension (1)
- High resolution-continuum source-graphite furnace molecular absorption spectrometry (HR-CS-GFMAS) (1)
- Historic buildings (1)
- Human plasma (1)
- IAQ (1)
- ISO22196 (1)
- Implants (1)
- In-vivo mutagenesis (1)
- Iodine (1)
- Ir DNA staining approach (1)
- Iron oxide nanoparticles (1)
- Korrosion (1)
- LCA (1)
- LIBS (1)
- Laser structuring (1)
- Laser-induced pariodic surface structures (1)
- Loading factor (1)
- Lysimeter (1)
- MDG ICP-ToF-MS (1)
- MIC projekt (1)
- Manuscripts (1)
- Material degradation (1)
- Mathematical modelling (1)
- Medieval glasses (1)
- Metal organic frameworks (1)
- Method development (1)
- Micro plastic (1)
- Microbiological influenced corrosion MIC (1)
- Microbiologically Influrenced Corrosion (MIC) (1)
- Microcolonial fungi (1)
- Microdroplet generator (1)
- Microfluidics (1)
- Mikrobiell beeinflusste Korrosion (1)
- Mikroplastik (1)
- Mobile anodisation (1)
- Mobile application (1)
- Modelling (1)
- Modern painting (1)
- Mycotoxins (1)
- Nano particle (1)
- NanoSIMS (1)
- Nanomaterial (1)
- Nanomaterials (1)
- Nanoparticles (1)
- Nanopartikel (1)
- Nitrification inhibitor (1)
- Nitrogen (1)
- Non invasive analysis (1)
- Odour (1)
- Organo fluorine analysis (1)
- PAK (1)
- PP (1)
- PS (1)
- Papyrus (1)
- Parchment (1)
- Particle size (1)
- Particle size distributuion (1)
- Passive sampling (1)
- Per- and polyfluorinated alkyl substances (PFASs) (1)
- Perceived Intensity (1)
- Perfluorocarboxylic acid (PFCA) (1)
- Phosphonic acids (1)
- Phosphor (1)
- Phosphorus recycling (1)
- Pigment (1)
- Plant growth test (1)
- Plant-availability (1)
- Pollutant (1)
- Polyfluorinated alcohol (PFOH) (1)
- Polyglycerol (1)
- Polymer (1)
- Portland Cement (1)
- Poster presentation (1)
- Pot experiment (1)
- ProMoAM (1)
- Proficiency Test (1)
- Protein a (1)
- Protein hydrolysis (1)
- Protein immobilization (1)
- Protein quantification (1)
- Prozessmonitoring (1)
- Pseudomonas (1)
- QA/QC (1)
- Reactive oxygen species (1)
- Recycling (1)
- Red Mud (1)
- Reductive amination (1)
- Reductive iron assimilation (1)
- Remediation (1)
- Reverse painting on glass (1)
- Rock-inhabiting fungi (1)
- Rostasche (1)
- Rotschlamm (1)
- SDS-PAGE (1)
- SPE extraction (1)
- Sapphire (1)
- Schutzverglasung (1)
- Schwermetalle (1)
- Selection (1)
- Sewage extraction (1)
- Sewage sludge (1)
- Sewage sludge ash (1)
- Siderophore (1)
- Signal transduction (1)
- Single cell (1)
- Sintering (1)
- Size distribution (1)
- Small angle x-ray scattering (1)
- Soil (1)
- Soil P species (1)
- Soil extraction (1)
- Solubility (1)
- Spectroscopy (1)
- Stained glass windows (1)
- Stained glasses (1)
- Standardized test and quantification procedure (1)
- Steelmaking slag (1)
- Structural color (1)
- Sub-4nm particles (1)
- Sugarcane bagasse (1)
- Sum parameter analyis (1)
- Superhydrophilic surface (1)
- Superhydrophobic surface (1)
- Surfacewaters (1)
- Synchrotron (1)
- Synchrotron-XPS (1)
- Synthetic organic pigments (1)
- TED-GC/MS (1)
- TEM (1)
- TXRF (1)
- TXRF ambient air aerosol chemical analysis aerosol element composition (1)
- Termites (1)
- Test Guideline (1)
- Testguideline (1)
- Thermal extraction (1)
- Thermochemisch (1)
- Transmission electron microscopy (1)
- Tricalcium-silicate (1)
- Tyrosine (1)
- UFP (1)
- Ultrafine particles (1)
- Ultrashort PFAS (1)
- Ultrashort laser processing (1)
- Umweltsimulation (1)
- Uptake rate (1)
- VOC-Emission (1)
- VOC-emission (1)
- Velocimetry (1)
- Volatile organic compound (1)
- Weathering (1)
- X-ray adsorption near-edge structure (XANES) spectroscopy (1)
- X-rays (1)
- XRF Analysis (1)
- Zeolite (1)
- biocide tolerance (1)
- black fungi (1)
- disinfection (1)
- evolution (1)
- fs-laser processing (1)
- genetics (1)
- genomics (1)
- heterogeneity (1)
Organisationseinheit der BAM
- 4 Material und Umwelt (146)
- 4.1 Biologische Materialschädigung und Referenzorganismen (62)
- 4.2 Material-Mikrobiom Wechselwirkungen (24)
- 6 Materialchemie (21)
- 4.0 Abteilungsleitung und andere (18)
- 4.5 Kunst- und Kulturgutanalyse (17)
- 4.3 Schadstofftransfer und Umwelttechnologien (16)
- 4.4 Thermochemische Reststoffbehandlung und Wertstoffrückgewinnung (14)
- 6.2 Material- und Oberflächentechnologien (14)
- 1 Analytische Chemie; Referenzmaterialien (12)
Black microcolonial fungi represent a group of ascomycetes with similar adaptations for existing in natural and anthropogenically created extreme habitats. They exhibit slow yeast-like or meristematic growth, do not form specialized reproduction structures and accumulate the black pigment 1,8-dihydroxynaphthalene (DHN) in the multilayered cell walls. We chose the rock inhabitant Knufia petricola of the Chaetothyriales as a representative for developing methods for genetic manipulation, simulation of mineral weathering and study of symbiotic interactions. Here, we report on the expansion of the genetic toolkit by more efficient multiplex CRISPR/Cas9 using a plasmid-based system for expression of Cas9 and multiple sgRNAs and three additional resistance selection markers. The targeted integration of expression constructs by replacement of essential genes for pigment synthesis allows for an additional color screening of the transformants. The black-pink screening due to the elimination of pks1 (melanin) was applied for promoter studies using GFP fluorescence as reporter, while the black-white screening due to the concurrent elimination of pks1 (melanin) and phs1 (carotenoids) was used to identify transformants that contain the two expression constructs for co-localization or bimolecular fluorescence complementation (BiFC) studies. In addition, two intergenic regions (igr1, igr2) were identified in which expression constructs can be inserted without causing obvious phenotypes. Plasmids of the pNXR-XXX series (Schumacher, 2012) and new compatible entry plasmids were used for fast and easy generation of expression constructs and are suitable for use in other fungal systems as well.
Antimicrobial resistance (AMR) is an important global health problem. The environment has been regocnized as an improtant compartment for the occurance, evolution and transmission of AMR. Biocides used as material preservatives are in contact with the environment and natural microbial communities through direct application and passive leaching from protected materials. It has been shown that environmental contaminants, such as antibiotics, metals and pesticides, can affect resistance evolution and spread by modifying the underlying processes of de novo mutagenesis, horizontal gene transfer and selection. However, it is unknown if material preservatives are involved in these processes and thereby can also drive AMR in the environment. Here, we investigate the effect of material preservatives on rates of de novo mutation and horizontal gene transfer (HGT) in Escherichia coli and the model soil microorganisms Acinetobacter baylyi and Bacillus subtilis. To this end, we determined the effects of material preservatives on the mutation rates and HGT with the Luria-Delbrück fluctuation assay and a conjugation assay with the broad host-range plasmid pKJK5 and a transformation assay. Our data shows that the quaternary ammonium compound DDAC, copper, the pyrethroid insecticide permethrin and the azole fungicide propiconazole significantly increase mutation rates in E. coli, whereas A. baylyi and B. subtilis are not significantly affected. Moreover, we show that the carbamate IPBC and the insecticide permethrin affect HGT in a concentration dependent manner. Investigations with reporter strains for bacterial stress response pathways show that induction of the general stress response (rpoS) and components of the SOS response (recA) underlie the effects of most biocides on mutation rates and HGT. Taken together, our data is important for assessing the contribution of biocides on AMR evolution and spread in the environment.
ICP-ToF (time of flight) MS enables the analysis of the multi-element fingerprint of single cells. The single cell ICP-ToF-MS is used in the presented poster for the analysis of archaea involved in microbiologically influenced corrosion (MIC) of steel. By means of sc-ICP-ToF-MS, the possible uptake of individual elements from the respective steel is investigated - the information obtained will be used in the future to elucidate underlying mechanisms and develop possible material protection concepts. The work combines modern methods of analytical sciences with materials.
Per- and polyfluoralkyl surfactants (PFAS) are industrially produced surface chemicals used in daily applications that have gained public and political attention due their unnaturally high appearance in drinking water, nourishments and soils.[1] Their chemical structures exhibit both lipophilic and hydrophilic properties, leading to a highly inert and persistent character.[2] Various PFAS have shown to be bioaccumulative in plants and animals, and some have been characterized as highly toxic when ingested.[3] When exposed to the environment, PFAS slowly get mobilized by natural water resources, leading to contamination of large areas of soil and natural water sources.[4] While PFAS contamination of drinking water has been investigated intensively, perfluoralkyl contamination of soils has been rarely examined as of yet. At the same time, an increasing amount of PFAS contamination sites are being discovered worldwide, calling for a sophisticated strategy towards analytical characterization.[5] Since the number of known PFAS already exceeds 4700, the established sum parameters like extractable organic fluorine (EOF) and adsorbable organic fluorine (AOF) are key elements to fully survey the impact of exposure.[6] Simultaneously, innovative soil remediation strategies are required to contain environmental destruction and to minimize further spreading of contaminants.[7] Addressing these challenges requires suitable analytical devices that are capable of mobilizing PFAS in the solid as well as in the liquid phase. The use of combustion ion chromatography (CIC) enables analysis of both immobile and volatile PFAS and allows the detection of both total fluorine (TF) and EOF or AOF of a given soil or aqueous sample. Based on preliminary results, the clean-up of a PFAS contaminated matrix is monitored via EOF detection over time. Additionally, we tested the effectiveness of urea (CH₄N₂O) as fluoride scavenger with the aim to improve the separation of inorganic and organic fluorine and therefore, to improve AOF accuracy. The presented findings can be contributive with regard to future AOF/EOF sum parameter development and application.
Biocides used as disinfectants are important to prevent the transmission of pathogens, especially during the current antibiotic resistance crisis. This crisis is exacerbated by phenotypically tolerant persister subpopulations which can survive transient antibiotic treatment and facilitate resistance evolution. Despite the transient nature of disinfection, knowledge concerning persistence to disinfectants and its link to resistance evolution is currently lacking. Here, we show that E. coli displays persistence against a widely used disinfectant benzalkonium chloride (BAC). Periodic, persister-mediated failure of disinfection rapidly selects for BAC tolerance. BAC tolerance is associated with reduced cell surface charge and mutations in the novel tolerance locus lpxM. Moreover, the fitness cost incurred by BAC tolerance turned into a fitness benefit in the presence of antibiotics, suggesting a selective advantage of BAC-tolerant mutants in antibiotic environments. Our findings provide a mechanistic underpinning for the faithful application of disinfectants to prevent multi-drug-resistance evolution and to steward the efficacy of biocides and antibiotics.
Biocides used as disinfectants are important to prevent the transmission of pathogens, especially during the current antibiotic resistance crisis. This crisis is exacerbated by phenotypically tolerant persister subpopulations which can survive transient antibiotic treatment and facilitate resistance evolution. Despite the transient nature of disinfection, knowledge concerning persistence to disinfectants and its link to resistance evolution is currently lacking. Here, we show that E. coli displays persistence against a widely used disinfectant benzalkonium chloride (BAC). Periodic, persister-mediated failure of disinfection rapidly selects for BAC tolerance. BAC tolerance is associated with reduced cell surface charge and mutations in the novel tolerance locus lpxM. Moreover, the fitness cost incurred by BAC tolerance turned into a fitness benefit in the presence of antibiotics, suggesting a selective advantage of BAC-tolerant mutants in antibiotic environments. Our findings provide a mechanistic underpinning for the faithful application of disinfectants to prevent multi-drug-resistance evolution and to steward the efficacy of biocides and antibiotics.
Antimicrobials can exert specific physiological effects when used in combination that are different from those when applied alone. These effects include physiological effects (i.e. synergy, antagonism and suppression) as well as evolutionary effects on the selection of resistant strains (i.e. cross-resistance and collateral sensitivity). While combination effects have been extensively mapped for antibiotic-antibiotic combinations, the combination effects of antibiotics with antimicrobials used as biocides or antiseptics have not been systematically investigated.
Here, we investigated the physiological and evolutionary consequences of combinations of antibiotics (meropenem, gentamicin and ciprofloxacin) and substances used as biocides or antiseptics (octenidine, benzalkonium chloride, cetrimonium bromide, chlorhexidine, povidone-iodine, silver) on growth and selection of Pseudomonas aeruginosa. We find prevalent physiological combination effects with synergy occurring 6 times and antagonism occurring 10 times. The effects are specific to the antibiotic-biocide combination with meropenem showing a tendency for antagonism with biocides (6 of 7), while gentamicin has a tendency for synergy (5 of 7). A particular strong antagonism is apparent for the meropenem-chlorhexidine combination, for which we conducted an in-depth study on the underlying molecular mechanism using RNASeq. Moreover, we find widespread effects of the biocide-antibiotic combinations on selection of P. aeruginosa strains resistant to the antibiotics, including cross-resistance and collateral sensitivity.
In conclusion, antibiotics and biocides or antiseptics exert physiological and evolutionary combination effects on the pathogen P. aeruginosa. These effects have consequences for the efficacy of both types of substances and for the selection of antimicrobial resistant strains in clinical applications with combined exposure (e.g. wound care, coated biomaterials).
ICP-ToF (Flugzeitanalysator, engl. time of flight)-MS ermöglicht den Multielement Fingerabdruck einzelner Zellen (single cell) zu analysieren. Die single cell-ICP-ToF-MS kommt bei dem vorgestellten Poster bei der Analyse von Archaeen, die an mikrobiell beeinflusster Korrosion (engl. microbiologically influenced corrosion, MIC) von Stahl eine Rolle spielen, zum Einsatz. Mittels sc-ICP-ToF-MS wird die mögliche Aufnahme von einzelnen Elementen aus dem jeweiligen Stahl untersucht – die erhaltenen Informationen fließen zukünftig in die Aufklärung zugrunde liegender Mechanismen sowie Entwicklung möglicher Materialschutzkonzepte ein. Die Arbeiten Verknüpfen moderne Methoden der Analytical Sciences mit Materialien.
Inter-pulse accumulation of heat could affect the chemical and morphological properties of the laser processed material surface. Hence, the laser pulse repetition rate may restrict the processing parameters for specific laser-induced surface structures. In this study, the evolution of various types of laser-induced micro- and nanostructures at various laser fluence levels, effective number of pulses and at different pulse repetition rates (1 – 400 kHz) are studied for common metals/alloys (e.g. steel or titanium alloy) irradiated by near-infrared ultrashort laser pulses (925 fs, 1030 nm) in air environment. The processed surfaces were characterized by optical and scanning electron microscopy (OM, SEM), energy dispersive X-ray spectroscopy (EDX) as well as time of flight secondary ion mass spectrometry (TOF-SIMS). The results show that not only the surface morphology could change at different laser pulse repetition rates and comparable laser fluence levels and effective number of pulses, but also the surface chemistry is altered. Consequences for medical applications are outlined.