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Erscheinungsjahr
- 2012 (2) (entfernen)
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- Englisch (2)
Schlagworte
- Alexa dyes (1)
- BSA (1)
- Bio-interface (1)
- Fluorescent particles (1)
- Nanobiotechnology (1)
- Nanoparticles (1)
- Nanosilica hybrids (1)
- PEG (1)
- Protective shell (1)
- Protein corona (1)
A facile and versatile synthetic route for controlling the size and surface potential of organic–inorganic hybrid silica nanoparticles (NPs) is introduced in this paper. For polymer-grafted NPs, the density of polymer chains on the surface is strongly affected by the concentration of precursor. Nevertheless, for condensed NPs, the precursor concentration determines the particle size but not the density of polymer chains on the surface or the adsorption of bovine serum albumin (BSA). Results presented here may have Major implications in biomedical and colloidal chemistry since interfacial and colloidal properties are known to drive several processes associated with nanoparticles in biological media.
Synthesis and characterisation of highly fluorescent core-shell nanoparticles based on Alexa dyes
(2012)
Current and future developments in the
emerging field of nanobiotechnology are closely
linked to the rational design of novel fluorescent
nanomaterials, e.g. for biosensing and imaging applications.
Here, the synthesis of bright near infrared
(NIR)-emissive nanoparticles based on the grafting
of silica nanoparticles (SNPs) with 3-aminopropyl
triethoxysilane (APTES) followed by covalent attachment
of Alexa dyes and their subsequent shielding by an
additional silica shell are presented. These nanoparticles
were investigated by dynamic light scattering (DLS),
transmission electron microscopy (TEM) and fluorescence
spectroscopy. TEM studies revealed the monodispersity
of the initially prepared and fluorophorelabelled
silica particles and the subsequent formation of
raspberry-like structures after addition of a silica
precursor.Measurements of absolute fluorescence quantum
yields of these scattering particle suspensions with
an integrating sphere setup demonstrated the influence
of dye labelling density-dependent fluorophore aggregation
on the signaling behaviour of such nanoparticles.