Filtern
Erscheinungsjahr
Dokumenttyp
- Zeitschriftenartikel (40)
- Beitrag zu einem Tagungsband (14)
- Forschungsbericht (6)
- Sonstiges (3)
- Beitrag zu einem Sammelband (2)
- Buchkapitel (1)
- Vortrag (1)
Schlagworte
- Brückenbeläge (4)
- Fahrbahnübergänge (4)
- Asphaltbauweisen (3)
- ELISA (3)
- Ethinylestradiol (3)
- Baustoffe (2)
- CZE (2)
- LCA (2)
- LCC (2)
- LCWE (2)
- Mass spectrometry (2)
- MoS2 (2)
- Mueller-Matrix imaging ellipsometry (2)
- Recycling (2)
- Spectroskopic Imaging (2)
- Standard measurement procedure (2)
- Structural health monitoring (2)
- Sustainability (2)
- Tribologie (2)
- 13C-NMR (1)
- 3D ultrastructural analysis (1)
- Absolute dose (1)
- Abwasser (1)
- Acquired immunodeficiency syndrome (1)
- Adsorption-desorption (1)
- Amino acid analysis (1)
- Amorphization (1)
- Analysis and design methods (1)
- Anforderungs- und Leistungsrichtlinien (1)
- Anti-counterfeiting (1)
- Antibiotika (1)
- Antibody (1)
- Antibody quantification (1)
- Antikörper (1)
- Azithromycin (1)
- BSA (1)
- Bauwerksüberwachung (1)
- Belastungstest (1)
- Betonstraßen (1)
- Biofuel (1)
- Biomarker (1)
- Biosensor (1)
- Bioseparation (1)
- Bisphenol A (1)
- Blasenkrebs (1)
- Bridge (1)
- Brillouin sensing (1)
- CCQM-K126 (1)
- CD104 (1)
- CD4 expression level (1)
- CD4+ cell counting (1)
- Calibration (1)
- Carbon fiber reinforced polymers (1)
- Cellular internalization (1)
- Cellular trafficking (1)
- Clarithromycin (1)
- Compost (1)
- Condition survey (1)
- Confocal laser scanning microscopy (1)
- Conjugates (1)
- Corrosion (1)
- Cowpea mosaic virus (1)
- Cryo-soft X-ray tomography (1)
- Cryogenic temperatures (1)
- Crystallization (1)
- Cytoplasmic remodeling (1)
- DIN 50989, Teile 1 bis 6 (1)
- DIN EN ISO/IEC 17025:2018 und GUM Kompatibilität (1)
- Damage (1)
- Dehnfugenkonstruktionen (1)
- Demolition material (1)
- Dendritic polyglycerol sulfate (1)
- Desert ecology (1)
- Deterioration (1)
- Development (1)
- Diclophenac (1)
- Digital model (1)
- Dirithromycin (1)
- Distributed sensing (1)
- Durability (1)
- Dynamic Bayesian Networks (1)
- EDTA (1)
- EE2 (1)
- Eddy current (1)
- Electrochemistry (1)
- Elektrochemisch (1)
- Elektrochemischer Sensor (1)
- Ellipsometric encoding (1)
- Ellipsometrie (1)
- Ellipsometrie-Norm (1)
- Ellipsometry (1)
- Endocrine disruption (1)
- Endokrine Disruptoren (1)
- Endokriner Disruptor (1)
- Engineering structure (1)
- Enzyme-Linked Immunosorbent Assay (1)
- Equivalent fluorescein fluorophore (EFF) (1)
- Erythromycin (1)
- Expert system (1)
- Extremophile (1)
- FIB/SEM slice and view (1)
- FITC (1)
- FT-IR (1)
- Fabry-Perot Schichten (1)
- Fabry-Perot layer stacks (1)
- Fabry-Perot layers (1)
- Fatigue (1)
- Femtosecond laser (1)
- Festschmierstoffe (1)
- Fiber-optic sensing (1)
- Flow cytometry (1)
- Fluorescence (1)
- Fluorescence Polarization (1)
- Forecast (1)
- Freisetzung (1)
- Fugen (1)
- Fugenfüllsysteme (1)
- Ganzheitlichkeit (1)
- Gastric cancer (1)
- Germany (1)
- Grain boundary precipitates (1)
- Hapten synthesis (1)
- Heat treatment (1)
- Hormone (1)
- Human immunodeficiency virus-1 (1)
- Humidity resistance (1)
- Hydroliquefaction (1)
- Hyperarid (1)
- Immunoassay (1)
- Impedanz (1)
- Inconel 718 (1)
- Inspection (1)
- Inspection planning (1)
- Kinetics (1)
- Konjugate (1)
- Kryogene Temperaturen (1)
- Kunstrasen (1)
- Kunststoffsportbeläge (1)
- Laves phase (1)
- Ligand analysis (1)
- Lignin (1)
- Load tests (1)
- Luftfeuchte (1)
- Lyophilized cells (1)
- MALDI-TOF-MS (1)
- MALDI-ToF (1)
- Macrolide antibiotics (1)
- Macrophage (1)
- Magnetic particles (1)
- Makrolide (1)
- Masonry (1)
- Mauerwerkbruch (1)
- Measurement of uncertainty (1)
- Measurement uncertainty (1)
- Messunsicherheit ellipsometrischer Messungen (1)
- Metabolomics (1)
- Metal additive manufacturing (1)
- Metal mobility (1)
- Metrology (1)
- Mikroplastik (1)
- MoS2-Beschichtungen (1)
- MoS2-coatings (1)
- Model update (1)
- Modell-Update (1)
- Moisture (1)
- Monitoring general (1)
- Monoklonale Antikörper (1)
- Monosaccharide (1)
- Mud volcanoe fluids (1)
- Nachhaltigkeit (1)
- Nano particles (1)
- Nanoparticles (1)
- Non-destructive testing (1)
- Offshore steel structures (1)
- Oleandomycin (1)
- Organic amendments (1)
- Organic matter charcterization (1)
- PVD (1)
- PVD-process (1)
- Peak identification (1)
- Peptide analysis (1)
- Pharmazeutika (1)
- Phenols (1)
- Photoimmunoconjugate (1)
- Photoimmunotherapy (1)
- Polarization spectroscopy (1)
- Polyethylenimine (1)
- Prestressed concrete (1)
- Product authentification (1)
- Produkt- und Markenschutz (1)
- Propability (1)
- Prototype (1)
- Quantitative flow cytometry (1)
- Quantum dots (1)
- Reference cell material (1)
- Reference material (1)
- Reinforced concrete (1)
- Reinforcement (1)
- Relative concentration measurement (1)
- Reliability (1)
- Research report (1)
- Residual stress (1)
- Rinderserumalbumin (1)
- Risk (1)
- Round robin test (1)
- Roxithromycin (1)
- Schadstofftransfer (1)
- Sensor-equipped geosynthetics (1)
- Serial sectioning (1)
- Sewage sludge (1)
- Silicon (1)
- Software (1)
- Soil (1)
- Soil amendment (1)
- Soils (1)
- Solid-phase synthesis (1)
- Spatial deterioration (1)
- Spectroscopic imaging ellipsometry (1)
- Stability (1)
- Stochastic process (1)
- Strain and temperature measurements (1)
- Structural Health Monitoring (1)
- Structural anomalies (1)
- Structural inhomogenities (1)
- Structural reliability (1)
- Structural system identification (1)
- Strukturmonitoring (1)
- Surface labelled lyophilized PBMC (1)
- Therapeutische Antikörper (1)
- Thermography (1)
- Thin films (1)
- Thiram (1)
- Toxikologie (1)
- Transformationsprodukte (1)
- Tribology (1)
- Two electrophoretic forms (1)
- UAS (1)
- UV Bestrahlung (1)
- Ultrasound (1)
- Umweltschadstoffe (1)
- Umweltverträglichkeit (1)
- Unbalanced magnetron sputtering (1)
- Validierungskonzepte ellipsometrischer Messungen (1)
- Wasserstofftechnologie (1)
- Water stable isotope analysis (1)
- X-ray computed tomography (1)
- X-ray laminography (1)
- X-ray radiography (1)
- ZIF (1)
- Zebrafisch (1)
- technische und technologische Anforderungen (1)
- Östrogene (1)
Organisationseinheit der BAM
- 1 Analytische Chemie; Referenzmaterialien (8)
- 1.8 Umweltanalytik (7)
- 7 Bauwerkssicherheit (7)
- 6 Materialchemie (5)
- 6.7 Materialsynthese und Design (4)
- 7.1 Baustoffe (4)
- 4 Material und Umwelt (3)
- 1.5 Proteinanalytik (2)
- 4.1 Biologische Materialschädigung und Referenzorganismen (2)
- 7.2 Ingenieurbau (2)
Paper des Monats
- ja (1)
Eingeladener Vortrag
- nein (1)
Hydroliquefaction is a possible pathway to produce liquid transportation fuels from solid feedstocks like coal or biomass. Though much effort has been put into the investigation of maximizing the oil yield using expensive catalysts and pasting oils in batch setups, little is known about how to commercialize the process. This work aims at the demonstration of lignin hydroliquefaction under conditions interesting for commercial operation. The results from hydroliquefaction experiments of two different lignin types using a cheap iron-based catalyst and anthracene oil as the pasting oil in a semibatch system are presented here. Oil yields of above 50% are reached without observing coke formation. Extensive analyses of the feedstocks and product oils were performed. The process supplies high-quality oil, while differences in the decomposition path of both lignin types are observed. An high heating value of 39 400 J/g and H/C and O/C ratios of up to 1.6 and 0.1, respectively, are detected for the produced bio-oils.
The synthetic estrogen ethinylestradiol (EE2) is an active component of oral contraceptives (OCs), considered as an endocrine disrupting compound (EDC). It is excreted from humans and released via sewage treatment plant effluents into aquatic environments. EDCs are any environmental pollutant chemical that, once incorporated into an organism, affects the hormonal balance of various species including humans. Its presence in the environment is becoming of great importance in water quality. This paper describes the development of an accurate, sensitive and selective method for capture, preconcentration and determination of EE2 present in water samples using: magnetic particles (MPs) as bioaffinity support for the capture and preconcentration of EE2 and a glassy carbon electrode modified with multi-walled carbon nanotubes (MWCNTs/GCE) as detection system. The capture procedure was based on the principle of immunoaffinity, the EE2 being extracted from the sample using the anti-EE2 antibodies (anti-EE2 Ab) which were previously immobilized on MPs. Subsequently the analyte desorption was done employing a sulfuric acid solution and the determination of the EE2 in the pre-concentrated solution was carried out by square wave voltammetry (SWV).
This method can be used to determine EE2 in the range of 0.03570 ng L-1 with a detection limit (LOD) of 0.01 ng L-1 and R.S.D. < 4.20%. The proposed method has been successfully applied to the determination of EE2 in water samples and it has promising analytical applications for the direct determination of EE2 at trace levels.
The aim of this work was to investigate the effects of long-term application of different organic fertilizers (sewage sludge, farmyard manure, compost) as compared to mineral fertilizer on the structure of the soil organic matter. Capillary electrophoresis was employed for the quantification of monosaccharides and phenolic compounds, whereas NMR and FT-IR were used for the overall characterization of the soils organic matter. Application of farmyard manure results in a higher content of organic matter derived from angiosperms, suggested by the higher levels of syringic and vanillic phenols. Spectroscopic studies show an increase of lignin and lignin-like products in the organic matter of the soil, which may be derived from the cereal straw supplied with farmyard manure. According to spectroscopic analysis, 13C CPMAS-NMR and FT-IR spectra, higher contents of methylene groups ( CH2) from proteins and protein-like compounds, as well as higher levels of carbohydrates, were found in the soil supplied with compost. The monosaccharide (rhamnose, xylose, glucose, mannose, arabinose, fucose and galactose) content was not significantly influenced by the different organic fertilizers, suggesting that the type of amendment used does not affect any of these six studied monomers. Comparing the three organic amendments the most significant differences were observed after long-term application of farmyard manure, with an increase in lignin and lignin-like products in the soil organic matter, and compost, which appears to contribute to an increase of protein and protein-like, as well as carbohydrates content on soil organic matter. The knowledge of such changes can be essential to understand the sorption and bioavailability of pollutants, as well as establish/unestablish ways to recycling organic residues as organic fertilizers.
A surface-labeled lyophilized lymphocyte (sLL) preparation has been developed using human peripheral blood mononuclear cells prelabeled with a fluorescein isothiocyanate conjugated anti-CD4 monoclonal antibody. The sLL preparation is intended to be used as a reference material for CD4+ cell counting including the development of higher order reference measurement procedures and has been evaluated in the pilot study CCQM-P102. This study was conducted across 16 laboratories from eight countries to assess the ability of participants to quantify the CD4+ cell count of this reference material and to document cross-laboratory variability plus associated measurement uncertainties. Twelve different flow cytometer platforms were evaluated using a standard protocol that included calibration beads used to obtain quantitative measurements of CD4+ T cell counts. There was good overall cross-platform and counting method agreement with a grand mean of the laboratory calculated means of (301.7 ± 4.9) µL-1 CD4+ cells. Excluding outliers, greater than 90% of participant data agreed within ±15%. A major contribution to variation of sLL CD4+ cell counts was tube to tube variation of the calibration beads, amounting to an uncertainty of 3.6%. Variation due to preparative steps equated to an uncertainty of 2.6%. There was no reduction in variability when data files were centrally reanalyzed. Remaining variation was attributed to instrument specific differences. CD4+ cell counts obtained in CCQM-P102 are in excellent agreement and show the robustness of both the measurements and the data analysis and hence the suitability of sLL as a reference material for interlaboratory comparisons and external quality assessment.
For the antiepileptic drug and anthropogenic marker carbamazepine (CBZ), a fast and cost-effective immunoassay based on fluorescence polarization (FPIA) was developed. The required fluorophore conjugates were synthesized from different fluorescein and CBZ derivatives. The most suitable tracer was CBZ–triglycine–5-(aminoacetamido)fluorescein. Additionally, the applicability of the assay in tubes and on microtiter plates was tested. The first format can be performed in a portable instrument and therefore can be applied in field measurements. The measurement of an individual sample can be carried out within 4 min. This assay shows a measurement range of 2.5–1000 µg L-1 and a test midpoint (or IC50) of 36 µg L-1. The FPIA performed on microtiter plates is useful for the assay development and is suitable for a very high throughput (up to 24 samples in 20 min). The test midpoint of this assay is 13 µg L-1 and the measurement range is 1.5–300 µg L-1. Furthermore, this assay requires smaller sample volumes and less reagents, including the crucial amount of antibody. The applicability of both assays to spiked surface water samples was evaluated. The recovery rates vary between 66–110% on microtiter plates and 81–140% in tubes.
This report focuses on the characterization of CD4 expression level in terms of equivalent number of reference fluorophores (ERF). Twelve different flow cytometer platforms across sixteen laboratories were utilized in this study. As a first step the participants were asked to calibrate the fluorescein isothiocyanate (FITC) channel of each flow cytometer using commercially available calibration standard consisting of five populations of microspheres. Each population had an assigned value of equivalent fluorescein fluorophores (EFF denotes a special case of the generic term ERF with FITC as the reference fluorophore). The EFF values were assigned at the National Institute of Standards and Technology (NIST). A surface-labelled lyophilized cell preparation was provided by the National Institute of Biological Standards and Control (NIBSC), using human peripheral blood mononuclear cells (PBMC) pre-labeled with a FITC conjugated anti-CD4 monoclonal antibody. Three PBMC sample vials, provided to each participant, were used for the CD4 expression analysis. The PBMC are purported to have a fixed number of surface CD4 receptors. On the basis of the microsphere calibration, the EFF value of the PBMC samples was measured to characterize the population average CD4 expression level of the PBMC preparations. Both the results of data analysis performed by each participant and the results of centralized analysis of all participants' raw data are reported. Centralized analysis gave a mean EFF value of 22,300 and an uncertainty of 750, corresponding to 3.3% (level of confidence 68%) of the mean EFF value. The next step will entail the measurement of the ERF values of the lyophilized PBMC stained with labels for other fluorescence channels. The ultimate goal is to show that lyophilized PBMC is a suitable biological reference cell material for multicolor flow cytometry and that it can be used to present multicolor flow cytometry measurements in terms of ABC (antibodies bound per cell) units.