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- CAD (3)
- Radiography (3)
- Dielectric heating (2)
- Holzschutz (2)
- Microwaves (2)
- Pest control (2)
- Radio waves (2)
- Radiographie (2)
- Termites (2)
- Acidobacteria (1)
- Antimicrobial (1)
- Archaea (1)
- Bankia sp (1)
- Bathyarchaeia (1)
- Bekämpfung (1)
- Cannibalism (1)
- Cockroaches (1)
- Computer modelling (1)
- Coptotermes (1)
- Core microbiome (1)
- Dielektrische Erwärmung (1)
- Doped systems (1)
- Durability (1)
- Efficacy (1)
- Entomopathogen (1)
- Environment (1)
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- Film (1)
- GDL (1)
- GNBP-2 (1)
- Gene expression (1)
- Gut microbiota (1)
- Heterogeneous catalysis (1)
- Hygienic behavior (1)
- Immunity (1)
- Insect biotechnology (1)
- Insecticide (1)
- Insekten (1)
- Kalman-Filter (1)
- Keratin (1)
- Lewis acid (1)
- Limnoria spp (1)
- Marine borers (1)
- Markov-Felder (1)
- Mathematical modeling (1)
- Metal fluoride (1)
- Metarhizium (1)
- Methanogens (1)
- Mikrowellen (1)
- Mikrowellentechnik (1)
- Millipedes (1)
- Modellierung (1)
- Modelling (1)
- Monte Carlo Modell (1)
- Monte Carlo Simulation (1)
- Monte Carlo Verfahren (1)
- Monte Carlo methods (1)
- Monte-Carlo (1)
- Natural products discovery (1)
- Nitrososphaerales (1)
- Nondestructive evaluation (1)
- Normung (1)
- Oberflächenmodelle (1)
- Operator of radiation transformation (1)
- Photon-electron transport (1)
- Proteome (1)
- Prozeßtomographie (1)
- RNA-Sequencing (1)
- Radiowellen (1)
- Radiowellentechnik (1)
- Rekonstruktionsalgorithmen (1)
- Selenium (1)
- Social (1)
- Social immunity (1)
- Social insects (1)
- Soldier (1)
- Spectrum reconstruction (1)
- Standardization (1)
- Statistische Estimationstheorie (1)
- Super somputing (1)
- Teredo sp (1)
- Termite (1)
- Termite-associated microbes (1)
- Tineola bisselliella (1)
- Tomography (1)
- Transcriptomics (1)
- Wood Protection (1)
- Wood protection (1)
- X-rays (1)
- underexplored phyla (1)
Organisationseinheit der BAM
Microbial communities in the immediate environment of socialized invertebrates can help to suppress pathogens, in part by synthesizing bioactive natural products. Here we characterized the core microbiomes of three termite species (genus Coptotermes) and their nest material to gain more insight into the diversity of termite-associated bacteria.
Sampling a healthy termite colony over time implicated a consolidated and highly stable microbiome, pointing toward the fact that beneficial bacterial phyla play a major role in termite fitness. In contrast, there was a significant shift in the composition of the core microbiome in one nest during a fungal infection, affecting the abundance of wellcharacterized Streptomyces species (phylum Actinobacteria) as well as less-studied bacterial phyla such as Acidobacteria. High-throughput cultivation in microplates was implemented to isolate and identify these less-studied bacterial phylogenetic group.
Amplicon sequencing confirmed that our method maintained the bacterial diversity of the environmental samples, enabling the isolation of novel Acidobacteriaceae and expanding the list of cultivated species to include two strains that may define new species within the genera Terracidiphilus and Acidobacterium.
Wood protection technology in the marine environment has changed over the last decades and will continue to do so. New active ingredients, newer formulations, and novel wood-based materials including physically- and chemically-modified wood, together with increasing concerns over environmental impacts of wood preservatives, urgently demand a major revision of EN 275 “Wood preservatives – Determination of the Protective Effectiveness against Marine Borers”, dated from 1992. This IRG document reports on the technical work in CEN TC 38 regarding the revision of this standard. A Task Group within WG 24 of CEN TC 38 was formed consisting of experts from different field of competence (e.g. wood preservatives industry, wood scientists, marine biologists, archaeologists and cultural heritage conservators). Starting by e-mail correspondence in 2014, and continuing with four physical meetings (Berlin 2x, Florence, Venice) with experts from Germany, Italy, Sweden, and UK were held so far. Significant items for revision in EN 275 were identified as: number of replicates, duration of the test, dimension of specimens, number of test sites, number of reference species, reference material including reference preservative, re-immersion of specimens after non-destructive periodical evaluation for longer periods of time vs higher number of replicates for successive destructive examinations without re-immersion, utilization of X- ray apparatus and specific software to ease evaluation, etc.
Furthermore, the task group is working on a standardized lab test for time-saving evaluation of different wood qualities for their potential to resist attack by limnorids. The suitability of this lab test will be determined by round robin tests as soon as safe face-to-face collaboration permits. The outcome will be published as a CEN TR (Technical Report) document, with a view to eventual adoption within the revised standard.
Chemical-free pest control by dielectric
heating with radio waves and microwaves:
Thermal effects
(2018)
Thermal pest control with hot air is widely accepted as an alternative to chemical methods. However, it requires relatively long treatment times owing to the low thermal conductivity of wood. Direct dielectric heating that applies radio waves or microwaves has the advantage of more homogeneous heating. However, Sound experimental data on this technique are currently rare. Therefore, the thermal treatment of wood-destroying insects with radio waves and microwaves was studied with two model pests, Anobium punctatum and Hylotrupes bajulus, and with Tenebrio molitor as a reference. The secure elimination of pests was achieved, and the corresponding treatment time was in the range of a few minutes. Temperature profiles were more homogeneous when applying radio waves.
Cr3+-doped MgF2 systems are synthesised by a novel non-aqueous soft chemistry route using different Cr precursors and varying Cr loading. These systems have been characterized by X-ray powder diffraction (XRD), BET surface area, TPD of ammonia, FT-IR pyridine adsorption analysis and X-ray photoelectron spectroscopy, and tested for their catalytic activity in dismutation of CCl2F2 and CF3CHClF. Catalysts synthesised starting from CrO3 or (CH3CO2)7Cr3(OH)2 showed better catalytic activity than those prepared with CrCl3. FT-IR pyridine adsorption studies reveal that the catalytic activity is highly correlated with the Lewis acid strength. In TPD of NH3 catalysts prepared from CrO3 and (CH3CO2)7Cr3(OH)2, precursors showed a similar behaviour, different from that of catalysts prepared from CrCl3.
Termite gut flagellates are colonized by host-specific lineages of ectosymbiotic and endosymbiotic bacteria. Previous studies have shown that flagellates of the genus Trichonympha may harbour more than one type of symbiont. Using a comprehensive approach that combined cloning of SSU rRNA genes with fluorescence in situ hybridization and electron microscopy, we investigated the phylogeny and subcellular locations of the symbionts in a variety of Trichonympha species from different termites. The flagellates in Trichonympha Cluster I were the only species associated with 'Endomicrobia', which were located in the posterior part of the cell, confirming previous results. Trichonympha species of Cluster II from the termite genus Incisitermes (family Kalotermitidae) lacked 'Endomicrobia' and were associated with endosymbiotic Actinobacteria, which is highly unusual. The endosymbionts, for which we suggest the name 'Candidatus Ancillula trichonymphae', represent a novel, deep-branching lineage in the Micrococcineae that consists exclusively of clones from termite guts. They preferentially colonized the anterior part of the flagellate host and were highly abundant in all species of Trichonympha Cluster II except Trichonympha globulosa. Here, they were outnumbered by a Desulfovibrio species associated with the cytoplasmic lamellae at the anterior cell pole. Such symbionts are present in both Trichonympha clusters, but not in all species. Unlike the intracellular location reported for the Desulfovibrio symbionts of Trichonympha agilis (Cluster I), the Desulfovibrio symbionts of T. globulosa (Cluster II) were situated in deep invaginations of the plasma membrane that were clearly connected to the exterior of the host cell.
Bekämpfungsverfahren gegen Organismen, die in Deutschland Bauholz wirtschaftlich bedeutend schädigen können, sind in der DIN 68800 Teil 4: 02/2012 „Holzschutz - Bekämpfungs- und Sanierungsmaßnahmen gegen Holz zerstörende Pilze und Insekten“ regeltechnisch zusammengefasst und gewichtet. Dabei wird je nach Verfahren und Schadorganismus zwischen Regelsanierung und Sondermaßnahmen unterschieden.
So wird z. B. bei den thermischen Maßnahmen im Sinne der Norm das sogenannte Heißluftverfahren gegen Insekten als Regelsanierung bezeichnet, wogegen es gegen Pilze nicht als solche ausgewiesen ist. Gleiches gilt für elektrophysikalische Verfahren sowohl gegen Pilze als auch gegen Insekten. Dies begründet sich dadurch, dass bei Erstellung der Norm Zweifel bezüglich weitgehender Allgemeingültigkeit und Praxisbewährtheit elektrophysikalischer Verfahren, wie sie zur Erwärmung des befallenen Holzes eingesetzt werden, bestanden. Somit erklärt sich auch, dass die Rahmenbedingungen einer erfolgreichen Bekämpfung mittels z. B. Mikrowellen denen des Heißluftverfahrens gleichgesetzt wurden, nämlich die vollständige Erwärmung des befallenen Holzbauteils auf eine Mindesttemperatur von 55° C für eine Dauer von mindestens 60 min. Die physikalisch-chemischen Vorteile der zeitgleichen Wärmegeneration im gesamten Holzkörper durch elektromagnetische Felder im Vergleich zum sukzessiven Wärmeeintrag durch Konduktion fanden keine Berücksichtigung.
The clothes moth Tineola bisselliella is one of a few insects that can digest keratin, leading to the destruction of clothing, textiles and artwork. The mechanism of keratin digestion is not yet fully understood, partly reflecting the lack of publicly available genomic and transcriptomic data. Here we present a high-quality gut transcriptome of T. bisselliella generated from larvae reared on keratin-rich and keratin-free diets. The overall transcriptome consists of 428,221 contigs that were functionally annotated and screened for candidate enzymes involved in keratin utilization. As a mechanism for keratin digestion, we identified cysteine synthases, cystathionine β-synthases and cystathionine γ-lyases. These enzymes release hydrogen sulfite, which may reduce the disulfide bonds in keratin. The dataset also included 27 differentially expressed contigs with trypsin domains, among which 20 were associated with keratin feeding. Finally, we identified seven collagenases that were upregulated on the keratin-rich diet. In addition to this enzymatic repertoire potentially involved in breaking down keratin, our analysis of poly(A)-enriched and poly(A)-depleted transcripts suggested that T. bisselliella larvae possess an unstable intestinal microbiome that may nevertheless contribute to keratin digestion
Die thermische Bekämpfung von Holzschädlingen erfordert wegen der geringen Wärmeleitfähigkeit von Holz lange Behandlungszeiten und kann zur Rissbildung führen. Hier wurde die thermische Behandlung mittels Radio- und Mikrowellen untersucht. Die direkte, dielektrische Erwärmung hat den Vorteil einer größeren Homogenität der Aufheizung. Die mit Radiowellen erzielten Temperaturprofile waren homogener als die mit Mikrowellen realisierten. Untersuchungen zeigten, dass die Abtötung nicht auf die direkte Einwirkung von elektromagnetischen Feldern, sondern auf die Temperaturerhöhung zurückzuführen ist.-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------Thermal pest control requires long treatment times due to the low thermal conductivity of wood and may lead to the formation of cracks. Here, the thermal treatment with radio waves as well as microwaves has been studied. The direct dielectric heating has the advantage of a good homogeneity. The obtained temperature profiles for radio waves were more homogeneous compared to microwaves. Detailed studies showed that elimination of pests was not related to the application of the electromagnetic field itself, but due to the temperature increase.
Modeling of the photon-electron cascade progress in multicomponent objects of complex geometrical structure by use of hybrid supercomputers is considered. An approach to computing the cascade processes is developed. The approach has three key properties allowing the effective use of heterogeneous structure of computers for solving the tasks of radiation transport in complex multi-scale geometries. Firstly, two different discreet geometrical description of an object being under radiation is used: triangulated model for photon transport and voxel model for electron transport. Secondly, small parameter of the problem is explicitly taking into account for modeling surface effects (for instance, electron emission). Thirdly, the effective calculation decomposition between CPU and GPU is developed for significant increasing the speed of calculations of processes in question. Modeling of experiment on researching the bremsstrahlung generated by electron beam in Ta target is carried out. Comparison of computing and experimental results shows satisfactory consent.
Computer modelling of non-destructive testing methods has come a long
way from the beginnings in the mid 90s to today. Radiographic modelling for
components with higher wall thicknesses, as they are typical for nuclear
applications, must include precise predictions of scattered radiation and its impact
in terms of contrast reduction. Dedicated or general purpose Monte Carlo methods
with the ability to calculate higher order scattering events are the state of the art for
these applications. Aerospace applications, on the other hand, have stronger
requirements on the modelling code's capabilities to import complex CAD
geometries, and can benefit from faster analytical scatter models, limited to first or
second order scattering events. Similar distinctions can be made for the various
approaches proposed to accurately model geometrical and film unsharpness, film
granularity, film responses, film/foil cartridges and photon noise. This article
presents a state-of-the-art review of radiographic modelling from the perspective of
two important application domains with very different requirements, nuclear and
aerospace.
The importance of soldiers to termite Society defence has long been recognized, but the contribution of soldiers to other societal functions, such as colony immunity, is less well understood.
We explore this issue by examining the role of soldiers in protecting nestmates against pathogen infection. Even though they are unable to engage in grooming behaviour, we find that the presence of soldiers of the Darwin termite, Mastotermes darwiniensis, significantly improves the survival of nestmates following entomopathogenic infection.
We also show that the copious exocrine oral secretions produced by Darwin termite soldiers contain a high concentration of Proteins involved in digestion, chemical biosynthesis, and immunity. The oral secretions produced by soldiers are sufficient to protect nestmates against infection, and they have potent inhibitory activity against a broad spectrum of microbes. Our findings support the view that soldiers may play an important role in colony immunity, and broaden our understanding of the possible function of soldiers during the origin of soldier-first societies.
Timber is constantly exposed to environmental influences under outdoor conditions which limits its Lifetime and usability. In order to counteract the damaging processes caused by insects, we have developed a novel and more environmentally friendly method to protect wood materials via covalent modification by organic insecticides. Starting with an important class of synthetic insecticides which are derived from the natural insecticide nicotine, various new carboxylic acid derivatives of imidacloprid were made accessible. These activated neonicotinoids were utilized for the chemical modification of wood hydroxy groups. In contrast to conventional wood preservation methods in which biocides are only physically bound to the surface for a limited time, the covalent fixation of the preservative guarantees a permanent effect against wood pests, demonstrated in standardized biological tests. Additionally, the environmental interaction caused by non-bound neonicotinoids is significantly reduced, since both, a smaller application rate is required and leaching of the active ingredient is prevented. By minimizing the pest infestation, the lifetime of the material increases while preserving the natural appearance of the material.
Social immune behaviors are described in a great variety of insect societies and their role in preventing emerging infectious diseases has become a major topic in insect research. The social immune system consists of multiple layers, ranging from the synthesis of external immune molecules to the coordination of individual behaviors into sophisticated collective defensive tasks. But our understanding of how complex group-level behavioral defenses are orchestrated has remained limited. We sought to address this gap in knowledge by investigating the relationship between the external activity of an important immune effector molecule in termites, Gram negative binding protein 2 (GNBP-2) and collective grooming and cannibalism. We reasoned that as an external enzyme capable of degrading entomopathogenic fungi, GNBP-2 can facilitate the spread of pathogenic molecules in the colony, and thus serve to trigger collective defenses in a manner analogous to pathogen-associated molecular signatures (PAMPs) of the individual immune system. To test whether GNBP-2 could play a role in regulating social immune behavior, we experimentally inhibited its fungicidal activity using the glycomimetic molecule, D-d-gluconolactone (GDL) and recorded collective behavioral responses to an infected nestmate. Contrary to expectations, GNBP-2 inhibition did not influence the rate or intensity of grooming of either control or fungus-infected
nestmates. By contrast, we found that the probability of being harmed through defensive cannibalistic behaviors was significantly reduced by the inhibition of GNBP-2. Our findings indicate that the regulation of collective immune behaviors may depend in part on the external secretion of an enzyme originating from the individual immune system, but that other cues are also necessary.
An approach is presented to construct operators for transforming the characteristics of incident radiation to transmitted radiation, as well as operators for transforming the transmitted radiation to measured values. Simu-lating the radiation transport is based on Monte Carlo modeling of the interaction of X-ray photons and electrons with matter. The proposed method permits to construct, for instance, the operator connecting the initial radiation spectrum with the absorbed photon energy penetrating a given object. The elaborated approach provides the possibility of effective mathematical modeling of radiation techniques such as radiography, treating complex multi-component objects. Moreover, the method can be used to construct the operator equation for solving in-verse problems, e.g. the reconstruction of the initial radiation spectrum using simple experimental measure-ments. Comparison with some experimental measurements is presented.
Methane emission by terrestrial invertebrates is restricted to millipedes, termites, cockroaches, and scarab beetles. The arthropod-associated archaea known to date belong to the orders Methanobacteriales, Methanomassiliicoccales, Methanomicrobiales, and Methanosarcinales, and in a few cases also to nonmethanogenic Nitrososphaerales and Bathyarchaeales. However, all major host groups are severely undersampled, and the taxonomy of existing lineages is not well developed. Full-length 16S rRNA gene sequences and genomes of arthropod-associated archaea are scarce, reference databases lack resolution, and the names of many taxa are either not validly published or under-classified and require revision. Here, we investigated the diversity of archaea in a wide range of methane-emitting arthropods, combining phylogenomic analysis of isolates and metagenome-assembled genomes (MAGs) with amplicon sequencing of full-length 16S rRNA genes. Our results allowed us to describe numerous new species in hitherto undescribed taxa among the orders Methanobacteriales (Methanacia, Methanarmilla, Methanobaculum, Methanobinarius, Methanocatella, Methanoflexus, Methanorudis, and Methanovirga, all gen. nova), Methanomicrobiales (Methanofilum and Methanorbis, both gen. nova), Methanosarcinales (Methanofrustulum and Methanolapillus, both gen. nova), Methanomassiliicoccales (Methanomethylophilaceae fam. nov., Methanarcanum, Methanogranum, Methanomethylophilus, Methanomicula, Methanoplasma, Methanoprimaticola, all gen. nova), and the new family Bathycorpusculaceae (Bathycorpusculum gen. nov.). Reclassification of amplicon libraries from this and previous studies using this new taxonomic framework revealed that arthropods harbor only CO2 and methyl-reducing hydrogenotrophic methanogens.
Numerous genus-level lineages appear to be present exclusively in arthropods,suggesting long evolutionary trajectories with their termite, cockroach, and millipede hosts, and a radiation into various microhabitats and ecological nichesprovided by their digestive tracts (e.g., hindgut compartments, gut wall, or anaerobic protists). The distribution patterns among the different host groups are often complex, indicating a mixed mode of transmission and a parallel evolution of invertebrate and vertebrate-associated lineages.