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- 2014 (4) (entfernen)
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- ELISA (2)
- 17alpha-ethinylestradiol (1)
- 17beta-estradiol (1)
- Abbildungsverfahren (1)
- Abwasser (1)
- Anthropogenic markers (1)
- Antikörper (1)
- BSA (1)
- Blasenkrebs (1)
- CD104 (1)
The synthesis of a novel PS conjugated with bovine and human serum albumin (BSA and HSA) and a monoclonal antibody anti-CD104 is reported, as well as their biological potential against the human bladder cancer cell line UM-UC-3. No photodynamic effect was detected when the non-conjugated porphyrin was used. Yet, when it was coupled covalently with the mAb anti-CD104, BSA and HSA, the resulting photosensitizer conjugates demonstrated high efficacy in destroying the cancer cells, the mAb anti-CD104 efficacy overruling the albumins.
This study comprises the development of an enzyme-linked immunosorbent assay (ELISA) for the quantification of caffeine in complex aqueous matrices without any sample clean-up procedure. Salinity and dissolved organic matter were selected as potential interfering agents. The addition of a sample buffer containing bovine serum albumin (BSA) prior to the sample was found to decrease the influence of those interfering agents. The working range of the developed method was 0.1–100 µg L- 1. Quantification of caffeine was possible in 43 out of 51 real aqueous samples, at values between < LOD and 15 µg L- 1. Results correlate well with those obtained by LCMS/MS. To the best of our knowledge this is the first study dealing with the quantification of caffeine in Portugal's surface waters.
Estrogens, such as 17β-estradiol (E2) and 17α-ethinylestradiol (EE2), are the major responsible for endocrine-disrupting effects observed in aquatic environments due to their high estrogenic potency, even at concentrations ranging from pg L-1 to ng L-1. Thus, it is essential to develop analytical methodologies suitable for monitoring their presence in water samples. Dispersive liquidliquid microextraction (DLLME) was used as a pre-concentration step prior to the quantification of E2 and EE2 by enzyme-linked immunosorbent assay (ELISA). First, an evaluation of the effect of DDLME on the E2 and EE2 ELISA calibration curves was performed. Since the extraction procedure itself had an influence on the ELISA optical density (OD), it became necessary to subject, not only the samples, but also all the standards to the DLLME process. Working ranges were determined, being between 1.2 and 8000 ng L-1, for E2, and between 0.22 and 1500 ng L-1, for EE2. The influence of organic matter, both in the extraction and quantification, was evaluated and it was observed that its presence in the solution did not affect considerably the calibration curve. Recovery rates were also determined, ranging from 77% to 106% for ultrapure water and from 104% to 115% for waste water samples, the most complex ones in what concerns matrix effects. Results obtained when applying the proposed method to real water samples can be considered quite satisfying. Moreover, the obtained working ranges encompass values generally reported in literature, confirming the practical use of the method for environmental samples.
Inhalt ist die zerstörungsfreie Detailuntersuchung von Holzbauteilen mit einem dreidimensional abbildenden Ultraschall-Echoverfahren. Das neu entwickelte 3D-Verfahren berücksichtigt den anisotropen Aufbau des Holzes. Die Bildschnitte und Projektionen werden aus flächigen Ultraschallaufnahmen berechnet. Die Messungen erfolgen an Probekörpern sowie an Bauteilen aus der Praxis. In dem Forschungsvorhaben wird die für Holz neue 3D-Rekonstruktionsrechnung verwendet und mit dem Ziel weiterentwickelt, unter Berücksichtigung der anisotropen Struktur des Holzes eine dreidimensional aufgelöste Abbildung von Reflektoren im Inneren der Holzbauteile zu ermöglichen. Damit werden die bisher für Holzbauteile zur Verfügung stehenden Verfahren entscheidend ergänzt, weil nun eine hochgenaue dreidimensionale Abbildung erreichbar ist (Auflösung: 20 mm und besser).