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Theoretical analysis of vibrating microstructuresusing Dynamic Scanning Electron Microscopy (DySEM)
(2015)
Tapping mode AFM (TM-AFM) is a standard technique to image biomolecules and cells avoiding damage to susceptible samples.
For preparing AFM samples we fixed (untreated and radiated) plasmids chemically on ultra-smooth mica silanized with APTES.
The recorded AFM images were examined concerning the contour length, the conformation and the writhing number of the plasmids.
We found that the measured contour length is in accordance with the number of base pairs. Apart from that, we indicate different structures of plasmids from our AFM images, which were assigned to our plasmid data from agarose gel-electrophoresis.