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Metaproteomics has matured into a powerful tool to assess functional interactions in microbial communities. While many metaproteomic workflows are available, the impact of method choice on results remains unclear. Here, we carry out a community-driven, multi-laboratory comparison in metaproteomics: the critical assessment of metaproteome investigation study (CAMPI). Based on well-established workflows, we evaluate the effect of sample preparation, mass spectrometry, and bioinformatic analysis using two samples: a simplified, laboratory-assembled human intestinal model and a human fecal sample. We observe that variability at the peptide level is predominantly due to sample processing workflows, with a smaller contribution of bioinformatic pipelines. These peptide-level differences largely disappear at the protein group level. While differences are observed for predicted community composition, similar functional profiles are obtained across workflows. CAMPI demonstrates the robustness of present-day metaproteomics research, serves as a template for multi-laboratory studies in metaproteomics, and provides publicly available data sets for benchmarking future developments.
The reuse of existing foundations is considered in various construction Projects to save time and costs while avoiding unnecessary interference with other underground objects. Because the design and as-built drawings might not be complete and questions may arise regarding the condition of the foundations, a detailed investigation is a prerequisite for the planning process in most cases. Nondestructive testing (NDT) techniques are a core part of this endeavor.
The processes and procedure for foundation reuse planning are not yet standardized, and the possibilities and limitations of NDT methods are not known to many planners and stakeholders. The German research Project REFUND (2014–2016) has developed charts for the planning and Investigation process that consider available standards and the current state of the art in NDT.
These charts are separately available for single and strip, slab, and pile foundations. Available NDT methods have been compiled and evaluated for specific tasks at these foundation types, including their respective limitations.
The procedures have been successfully tested in two real-world projects. The results from these projects enable planners to improve the reliability of the process while potentially saving significant resources. This paper discusses the procedures for pile foundations and demonstrates the use of various NDT methods in a project on the reuse of electrical tower foundations.
Zur Gründung von Bauwerken auf weichen, gering tragfähigen Böden werden unter anderem Betonpfähle, einzeln oder in Pfahlgruppen, eingesetzt. Deren Bewertung bzgl. Tragverhalten bzw. Integrität ist bei bestimmten Baugrundverhältnissen und Pfahlgeometrien schwierig. Hierfür werden statische und dynamische Pfahlprüfungen durchgeführt. Aus Messwerten vom Pfahlkopf bzw. vom oberen Pfahlbereich werden üblicherweise die notwendigen Aussagen abgeleitet. Eine genauere Bewertung ist möglich, wenn präzise Messwerte über die gesamte Pfahllänge vorliegen. Deshalb wurden hochauflösende betoneinbettbare Messwertaufnehmer auf Basis von Faser-Fabry-Perot-Sensoren entwickelt, die in Modell- und in realen Rammpfählen getestet wurden. Der Beitrag beschreibt den Sensor, die Installation und exemplarisch einige Testergebnisse zur Erfassung der Wellenausbreitung im Modellpfahl. Feldtests wurden zurzeit der Erstellung des Manuskripts erfolgreich begonnen.
Reinforced concrete piles are often used for structures on non-sufficiently bearing soil areas. Assessment of bearing capacity and bearing behaviour of large concrete piles remains a difficult task under specific soil conditions and pile geometries. Usually static and dynamic pile tests are carried out for quality assurance. From dynamic measurements taken only from the pile head, the bearing behaviour and structural integrity can be derived using the one-dimensional theory of wave propagation. More precise information about the pile features can be achieved by highly resolving fibre-optic sensors based on Fabry-Perot technology, distributed over several locations along the pile length. Small scale piles and real driven piles have been tested. This paper describes the sensor, the sensor installation, and experimental results of tests in laboratory as well as test results for recording the wave propagation in the small scale piles.