Filtern
Erscheinungsjahr
Dokumenttyp
- Zeitschriftenartikel (34)
- Beitrag zu einem Tagungsband (4)
- Forschungsbericht (2)
- Beitrag zu einem Sammelband (1)
- Vortrag (1)
Sprache
- Englisch (42) (entfernen)
Schlagworte
- Ellipsometry (5)
- Thin films (4)
- Gas sensing (3)
- Mass spectrometry (3)
- Antibody (2)
- Carbon steel (2)
- Cement (2)
- Cyclic voltammetry (2)
- DOTA (2)
- Doped tin oxide (2)
- Electrochromism (2)
- Ellipsometrie (2)
- Metal labeling (2)
- Metallo-supramolecular polyelectrolytes (2)
- Mueller-Matrix imaging ellipsometry (2)
- Optical properties (2)
- Spectroskopic Imaging (2)
- TMF (2)
- Tandem MS (2)
- Thermo-mechanical fatigue (2)
- Adhesion (1)
- Air-coupled (1)
- Airborne (1)
- Amorphization (1)
- Amorphous state (1)
- Anti-counterfeiting (1)
- Aquifer fluid (1)
- CCS (1)
- CID (1)
- Carbon (1)
- Carbon capture (1)
- Carbon capture storage (1)
- Carbon dioxide (1)
- Carbon-based Films (1)
- Casing (1)
- Certified reference material (1)
- Certified reference materials (1)
- Cisplatin (1)
- Coagulation (1)
- Coated/Layered Reference Materials (1)
- Coatings (1)
- Collision induced dissociation (CID) (1)
- Collision-induced dissociation (1)
- Colouration efficiency (1)
- Corrosion (1)
- Corrosion and storage (CCUS) technology (1)
- Crevice corrosion (1)
- Crystallization (1)
- DNA (1)
- Degradation (1)
- Depth profiling (1)
- Design for durability (1)
- Dip coating (1)
- Double strands (1)
- Electrochromic device (1)
- Fabry-Perot Schichten (1)
- Fabry-Perot layers (1)
- Fatigue damage (1)
- Fatigue life time (1)
- Femtosecond laser (1)
- Ferroelectret (1)
- Fragmentation (1)
- Fragmentation activation (1)
- GDS (1)
- Gadolinium-based contrast agents (GBCAs) (1)
- Glow discharge spectrometry (1)
- Glycans (1)
- HCD (1)
- Hydrogen (1)
- ICP-MS (1)
- ISO (1)
- Imaging ellipsometry (IE) (1)
- Impurities (1)
- Inhomogeneous and patterned thin films (1)
- Interlaboratory Comparison (1)
- Ion mobility (1)
- Ion mobility spectrometry (1)
- Iron doped tin oxide (1)
- Kohlenstoffschichten (1)
- Labeling (1)
- Lanthanide (1)
- Lanthanide DOTA (1)
- Lanthaniden (1)
- Laser ablation inductively coupled plasma–mass spectrometry imaging (LA-ICP-MSI) (1)
- Life time prediction (1)
- Locked nucleic acids (1)
- Macrocell corrosion model (1)
- Magnetron (1)
- Magnetron sputtering (1)
- Matrix-assisted laser desorption ionization–mass spectrometry imaging (MALDI-MSI) (1)
- Metal oxides (1)
- Metall-Markierung (1)
- Metrology (1)
- Multilayer (1)
- Multiplexing (1)
- Native MS (1)
- Ni-base superalloy (1)
- Nitrides (1)
- Oligonucleotide (1)
- Oligonucleotide fragmentation (1)
- Optical Property (1)
- Optical materials (1)
- Optical microscopy (OM) (1)
- PA-CVD (1)
- PTM (1)
- Patterned films (1)
- Photoresist (1)
- Pitting (1)
- Plasma collision processes (1)
- Plasma deposition (1)
- Plasmachemie (1)
- Plasmonic absorption (1)
- Polarization spectroscopy (1)
- Produkt- und Markenschutz (1)
- Protein quantification (1)
- Protein-Quantifizierung (1)
- Quantitative proteomics (1)
- Reagenzien (1)
- Referenced spectroscopic ellipsometry (RSE) (1)
- Refractive index (1)
- Reinforcement corrosion (1)
- Resonance (1)
- Round Robin (1)
- SPR effect (1)
- Scratch test (1)
- Sheet resistivity (1)
- SiO2 (1)
- Silicon (1)
- SnO2 (1)
- Spectroscopic ellipsometry (1)
- Spectroscopic imaging and mapping ellipsometry (1)
- Spectroscopic imaging ellipsometry (1)
- Stable isotope labeling (1)
- Standardisation (1)
- Structural anomalies (1)
- Structural inhomogenities (1)
- Surface plasmon (1)
- Surface plasmon resonance (1)
- Tandem-MS (1)
- Test methods (1)
- Thermo-Mechanical strain-controlled fatigue testing (1)
- Thermoacoustic (1)
- Transparent conductive oxides (1)
- Transparent conductive oxides (TCO) (1)
- Ultrasonic transducer (1)
- Utilization (1)
- Vacuum ultraviolet (VUV) light (1)
- Vanadium oxide (1)
- XAFS (1)
- XPS characterzation (1)
Organisationseinheit der BAM
- 1 Analytische Chemie; Referenzmaterialien (6)
- 1.9 Chemische und optische Sensorik (4)
- 6 Materialchemie (3)
- 6.7 Materialsynthese und Design (3)
- 5 Werkstofftechnik (2)
- 5.1 Mikrostruktur Design und Degradation (2)
- 7 Bauwerkssicherheit (2)
- 7.6 Korrosion und Korrosionsschutz (2)
- 1.1 Anorganische Spurenanalytik (1)
- 1.3 Instrumentelle Analytik (1)
Eingeladener Vortrag
- nein (1)
Besides protein identification via mass spectrometric methods, protein and peptide quantification has become more and more important in order to tackle biological questions. Methods like differential gel electrophoresis or enzyme-linked immunosorbent assays have been used to assess protein concentrations, while stable isotope labeling methods are also well established in quantitative proteomics. Recently, we developed metalcoded affinity tagging (MeCAT) as an alternative for accurate and sensitive quantification of peptides and
proteins. In addition to absolute quantification via inductively coupled plasma mass spectrometry, MeCAT also enables sequence analysis via electrospray ionization tandem mass spectrometry. In the current study, we developed a new labeling approach utilizing an iodoacetamide MeCAT reagent (MeCAT-IA). The MeCAT-IA approach shows distinct advantages over the previously used MeCAT with maleinimide reactivity such as higher labelling efficiency and the lack of diastereomer formation during labeling. Here, we present a careful characterization of this new method focusing on the labeling process, which yields complete tagging with an excess of reagent of 1.6 to 1, less complex chromatographic behavior, and fragmentation characteristics of the tagged peptides using the iodoacetamide MeCAT reagent.