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- Aerodynamic damping (2)
- Overhead transmission lines (2)
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Organisationseinheit der BAM
Biofilms are ubiquitous in nature and in the man-made environment. Given their harmful effects on human health, an in-depth understanding of biofilms and the monitoring of their formation and growth are important. Particularly relevant for many metabolic processes and survival strategies of biofilms is their extracellular pH. However, most conventional techniques are not suited for minimally invasive pH measurements of living biofilms. Here, a fluorescent nanosensor is presented for ratiometric measurements of pH in biofilms in the range of pH 4.5–9.5 using confocal laser scanning microscopy. The nanosensor consists of biocompatible polystyrene nanoparticles loaded with pH-inert dye Nile Red and is surface functionalized with a pH-responsive fluorescein dye. Its performance was validated by fluorometrically monitoring the time-dependent changes in pH in E. coli biofilms after glucose inoculation at 37 °C and 4 °C. This revealed a temperature-dependent decrease in pH over a 4-h period caused by the acidifying glucose metabolism of E. coli. These studies demonstrate the applicability of this nanosensor to characterize the chemical microenvironment in biofilms with fluorescence methods.
Background - It is known that dietary intake of polyunsaturated fatty acids may improve cardiac function. However, relatively high daily doses are required to achieve sufficient cardiac concentrations of beneficial omega‐3 fatty acids. The liver X receptor (LXR) is a nuclear hormone receptor and a crucial regulator of lipid homeostasis in mammals. LXR activation has been shown to endogenously reprogram cellular lipid profiles toward increased polyunsaturated fatty acids levels. Here we studied whether LXR lipid reprogramming occurs in cardiac tissue and exerts cardioprotective actions.
Methods and Results - Male 129SV mice were treated with the LXR agonist AZ876 (20 µmol/kg per day) for 11 days. From day 6, the mice were injected with the nonselective β‐agonist isoproterenol for 4 consecutive days to induce diastolic dysfunction and subendocardial fibrosis while maintaining systolic function. Treatment with isoproterenol led to a marked impairment of global longitudinal strain and the E/e' ratio of transmitral flow to mitral annular velocity, which were both significantly improved by the LXR agonist. Histological examination showed a significant reduction in isoproterenol‐induced subendocardial fibrosis by AZ876. Analysis of the cardiac lipid composition by liquid chromatography‐high resolution mass spectrometry revealed a significant increase in cardiac polyunsaturated fatty acids levels and a significant reduction in saturated fatty acids by AZ876.
Conclusions - The present study provides evidence that the LXR agonist AZ876 prevents subendocardial damage, improves global longitudinal strain and E/e' in a mouse model of isoproterenol‐induced cardiac damage, accompanied by an upregulation of cardiac polyunsaturated fatty acids levels. Cardiac LXR activation and beneficial endogenous cardiac lipid reprogramming may provide a new therapeutic strategy in cardiac disease with diastolic dysfunction.
The ternary sulfido bismuthate K3[BiS3] is synthesized in quantitative yields. The material exhibits nonlinear optical properties with strong second harmonic generation properties at arbitrary wavelengths in the infrared spectral range and a notable laser-induced damage threshold of 5.22 GW cm−2 for pulsed laser radiation at a wavelength of 1040 nm, a pulse duration of 180 fs, and a repetition rate of 12.5 kHz. K3[BiS3] indicates semiconductivity with a direct optical band gap of 2.51 eV. Dielectric and impedance characterizations demonstrate κ values in the range of 6−13 at 1 kHz and a high electrical resistivity. A strong diamagnetic behavior with a susceptibility of −2.73 × 10−4 m3 kg−1 at room temperature is observed. These results suggest it is a promising nonlinear optical candidate for the infrared
region. The synergic physical characteristics of K3[BiS3] provide insight into the correlation of optical, electrical, and magnetic properties.
Overhead transmission lines are very sensitive structures in regards to wind action. The cables, spanning over a few hundred meters contribute in particular to the overall action on the suspension towers. These slender structures incorporate both structural nonlinearities from the large deformation of the cables and aerodynamic nonlinearities which need to be accounted for when it is to estimate the system response to strong wind events. In this work, a finite element procedure is presented to model an existing power line section using nonlinear cable elements. The wind force is assumed quasi-steady with force coefficients determined in wind tunnel test on a conductor section. Further, aerodynamic damping is incorporated by considering the relative velocity between cable nodes and oncoming wind flow. The results are compared with on-site measurements of the cables support reaction. The results show a significant effect of damping since almost no resonant amplification is visible both in observation and simulation. In addition, wind tunnel tests approved aerodynamic damping to be large for the system of sagging cables, but nonlinear in its nature. It is concluded, that the dynamic response of overhead transmission line cables has to be modeled with care, considering all sources of nonlinearities. That is of particular interest in case of random excitation such as wind because the peak response depends on the probability distribution of the system's response.
Measurements of downburst wind loading acting on an overhead transmission line in Northern Germany
(2017)
Along an overhead transmission line in Northern Germany, a unique instrumentation of anemometers and force measurements is installed. Details of this test line with wind measurements along a horizontal axis are given. A recent event of a presumable downburst wind event is analyzed by means of available data and precedent works on thunderstorm analysis. The measured response of the conductors at the suspension tower is investigated and compared with time domain simulation of a finite element model.
Aerodynamic damping is a decisive parameter influencing the dynamic response of overhead transmission line conductors. Methods of how to account for the effects of aerodynamic damping differ significantly and so might do the results. In this work, the source of aerodynamic damping being the result of the relative velocity between the structure and wind flow will be revised. Based on wind tunnel tests and validated by simulations, the differences of linear movement compared to a pendulum movement of a sagging cable are shown. The reasons for that Deviation are the large deflections, resulting in a movement non-parallel to the acting wind flow. For analysis in frequency domain, it is not possible to incorporate aerodynamic damping implicitly by fluid structure interaction. If the dynamic movement can be linearized at a working point of the mean deflection, a modification to the linear approach is suggested. This approach is validated by simulation with a finite element model of an existing overhead transmission line, calibrated with full scale measurements. Aerodynamic damping is incorporated in time step analysis by Rayleigh damping and modal damping. The differences between both approaches are emphasized and modal damping is shown to be the most adequate.