Filtern
Dokumenttyp
- Zeitschriftenartikel (1)
- Sonstiges (1)
Sprache
- Englisch (2)
Schlagworte
- Antibodies (1)
- Antibody ID (1)
- Fluorescence (1)
- Gated hybrid material (1)
- Insecticide (1)
- Lateral flow test (1)
- Monoclonal (1)
- Open data (1)
- Peer review (1)
- Permethrin (1)
Organisationseinheit der BAM
A recent Opinion Article by Jan Voskuil (JV), Aeonian Biotech, discussed the challenges in validation of research antibodies. It was openly peer reviewed by Michael Weller (MW), Federal Institute for Materials Research and Testing (BAM), Germany. In this blog, both discuss these challenges, the reproducibility crisis and open data.
The employment of type-I pyrethroids for airplane disinfection in recent years underlines the necessity to develop sensing schemes for the rapid detection of these pesticides directly at the point-of-use. Antibody-gated indicator-releasing materials were thus developed and implemented with test strips for lateral-flow assay-based analysis employing a smartphone for readout. Besides a proper matching of pore sizes and gating macromolecules, the functionalization of both the material's outer surface as well as the strips with PEG chains enhanced system performance. This simple assay allowed for the detection of permethrin as a target molecule at concentrations down to the lower ppb level in less than 5 minutes.